Methylobacterium populi sp. nov., a novel aerobic, pink-pigmented, facultatively methylotrophic, methane-utilizing bacterium isolated from poplar trees (Populus deltoides×nigra DN34)

Aken, Benoı̂t Van; Peres, Caroline; Doty, Sharon L.; Yoon, Jong Moon; Schnoor, Jerald L.

doi:10.1099/ijs.0.02796-0

Cited by 222 publications

(115 citation statements)

References 36 publications

Supporting

Mentioning

106

Contrasting

Unclassified

Order By: Relevance

“…However, early reports that it could also grow on methane have proven difficult to reproduce (11). A recent taxonomic description of Methylobacterium populi also describes this species as methanotrophic (29), but this claim is not supported by sufficient data (11). In contrast to Methylobacterium, Methylocella has been demonstrated to possess both the genetic and enzymatic machineries of methane oxidation via sMMO (9,13,17), and methane uptake in pure culture can be demonstrated (Fig.…”

Section: Discussionmentioning

confidence: 96%

Methylocella Species Are Facultatively Methanotrophic

2005

View full text Add to dashboard Cite

All aerobic methanotrophic bacteria described to date are unable to grow on substrates containing carboncarbon bonds. Here we demonstrate that members of the recently discovered genus Methylocella are an exception to this. These bacteria are able to use as their sole energy source the one-carbon compounds methane and methanol, as well as the multicarbon compounds acetate, pyruvate, succinate, malate, and ethanol. To conclusively verify facultative growth, acetate and methane were used as model substrates in growth experiments with the type strain Methylocella silvestris BL2. Quantitative real-time PCR targeting the mmoX gene, which encodes a subunit of soluble methane monooxygenase, showed that copies of this gene increased in parallel with cell counts during growth on either acetate or methane as the sole substrate. This verified that cells possessing the genetic basis of methane oxidation grew on acetate as well as methane. Cloning of 16S rRNA genes and fluorescence in situ hybridization with strain-specific and genus-specific oligonucleotide probes detected no contaminants in cultures. The growth rate and carbon conversion efficiency were higher on acetate than on methane, and when both substrates were provided in excess, acetate was preferably used and methane oxidation was shut down. Our data demonstrate that not all methanotrophic bacteria are limited to growing on one-carbon compounds. This could have major implications for understanding the factors controlling methane fluxes in the environment.Aerobic methanotrophic bacteria occupy a key position in the global methane cycle. In aerobic interfaces of flooded soils and wetlands their activity limits potential methane efflux to the atmosphere, and in well-aerated upland soils they consume atmospheric methane directly (8, 25). The 11 described genera of methanotrophic bacteria include species with diverse environmental tolerances and biochemical properties, but one trait considered common to all is the inability to grow on substrates containing carbon-carbon bonds (5,18,20). Growth is limited to methane, methanol, and in some cases formate, formaldehyde, and methylamines (5). Experiments with 14 C-labeled substrates have shown that small amounts of some organic acids can also be assimilated during exponential growth on one-carbon substrates. However, this accounts for at most 5 to 10% of the total C assimilation, no energy is gained, and no growth occurs on these compounds alone (18).Recently, the new methanotrophic species Methylocella palustris (13, 14), Methylocella silvestris (17), and Methylocella tundrae (9) were isolated from acidic peat, forest, and tundra soils, respectively. Together with Methylocapsa acidiphila (12), these species form a distinct taxonomic cluster of acidophilic, methanotrophic bacteria. These belong to the Alphaproteobacteria (type II methanotrophs) but are not monophyletic with the previously known type II methanotrophs of the genera Methylosinus and Methylocystis. Instead, Methylocella species are closely related to the nonmeth...

show abstract

Section: Discussionmentioning

confidence: 96%

Methylocella Species Are Facultatively Methanotrophic

2005

View full text Add to dashboard Cite

show abstract

“…It includes strictly aerobic, Gram-negative, rod-shaped, pink-pigmented, facultatively methylotrophic (PPFM) bacteria, which can grow on single carbon compounds such as formate, formaldehyde and methanol as the sole source of carbon and energy, as well as on a wide range of multi-carbon growth substrates (Green, 2001). At the time of writing, the genus comprises 20 recognized species (Patt et al, 1976;Bousfield & Green, 1985;Green et al, 1988;Urakami et al, 1993;Wood et al, 1998;Doronina et al, 2000Doronina et al, , 2002McDonald et al, 2001;Van Aken et al, 2004;Jourand et al, 2004;Gallego et al, 2005a, b, c), with Methylobacterium organophilum as the type species (Patt et al, 1976).…”

mentioning

confidence: 99%

Methylobacterium adhaesivum sp. nov., a methylotrophic bacterium isolated from drinking water

Gallego

Garcı́a

Ventosa

2006

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

A pink-pigmented, facultatively methylotrophic strain, AR27 T , isolated from the drinking water distribution system of Seville, Spain, was characterized taxonomically. Cells were Gram-negative rods, motile, non-spore-forming and strictly aerobic. Growth in liquid media was flocculant whereas on solid media growth produced colonies that usually adhered to the agar surface. On the basis of its phenotypic characteristics, 16S rRNA gene sequence data and DNA G+C content (63?6 mol%), strain AR27 T (=CECT 7069 T =CCM 7305 T ) is proposed as the type strain of a novel species of the genus Methylobacterium, Methylobacterium adhaesivum sp. nov.The genus Methylobacterium belongs to the a-2 subclass of the Proteobacteria and has the serine pathway for formaldehyde assimilation. It includes strictly aerobic, Gram-negative, rod-shaped, pink-pigmented, facultatively methylotrophic (PPFM) bacteria, which can grow on single carbon compounds such as formate, formaldehyde and methanol as the sole source of carbon and energy, as well as on a wide range of multi-carbon growth substrates (Green, 2001). At the time of writing, the genus comprises 20 recognized species (Patt et al., 1976;Bousfield & Green, 1985;Green et al., 1988;Urakami et al., 1993;Wood et al., 1998; Doronina et al., 2000Doronina et al., , 2002 McDonald et al., 2001;Van Aken et al., 2004;Jourand et al., 2004; Gallego et al., 2005a, b, c), with Methylobacterium organophilum as the type species (Patt et al., 1976).Members of the genus Methylobacterium are ubiquitous in terrestrial habitats (Green & Bousfield, 1981, including soil, dust, freshwater, tap water systems, lake sediments, leaf surfaces and nodules, rice grains and air, and as contaminants in various products and processes. The PPFM bacteria are strict aerobes and can be isolated from almost any freshwater environment where some dissolved oxygen exists, e.g. from tap water systems (Gräf & Bauer, 1973) and from stratified lake systems (Hanson, 1980). Furthermore, species of this genus have been isolated from hospital environments (Gilardi & Faur, 1984), where they may pose a threat as opportunistic pathogens to seriously ill patients.A sampling campaign in April 2004 to identify culturable and non-culturable bacteria present in the drinking water distribution system of Seville, Spain, led to isolation of a slowly growing pink-pigmented bacterium. This isolate was studied phenotypically, phylogenetically and genotypically. Based on these characteristics we suggest that strain AR27 T represents a novel species of the genus Methylobacterium.Drinking water samples (25 l) were concentrated by using a tangential flow filtration system (Filtron), plated on plate count agar (PCA; Difco) and R2A (Difco) and incubated at 28 uC for 7 days. Distinct colonies were picked in order to obtain pure cultures. Pink-pigmented colonies were selected and studied in more detail. One of these strains, AR27 T , showed a characteristic growth that differentiated it from the remaining pink-pigmented colonies isolated. Strain AR27 ...

show abstract

“…The genus Methylobacterium was first described by Patt et al (1976) and at the time of writing consists of the following 24 recognized species (http://www.bacterio.cict.fr/m/methylobacterium. html): Methylobacterium adhaesivum (Gallego et al, 2006), M. aminovorans (Urakami et al, 1993), M. aquaticum (Gallego et al, 2005a), M. chloromethanicum (McDonald et al, 2001), M. dichloromethanicum (Doronina et al, 2000), M. extorquens (Bousfield & Green, 1985), M. fujisawaense (Green et al, 1988), M. hispanicum (Gallego et al, 2005a), M. isbiliense (Gallego et al, 2005c), M. jeotgali (Aslam et al, 2007), M. lusitanum (Doronina et al, 2002), M. mesophilicum , M. nodulans (Jourand et al, 2004), M. organophilum (Patt et al, 1976), M. oryzae (Madhaiyan et al, 2007), M. podarium (Anesti et al, 2004), M. populi (Van Aken et al, 2004), M. radiotolerans , M. rhodesianum (Green et al, 1988), M. rhodinum , M. suomiense (Doronina et al, 2002), M. thiocyanatum (Wood et al, 1998), M. variabile (Gallego et al, 2005b) and M. zatmanii (Green et al, 1988). Here, we provide a detailed taxonomic characterization of a Methylobacterium-like bacterial strain, PMB02 T , which was isolated from a tree leaf.…”

mentioning

confidence: 99%

Methylobacterium platani sp. nov., isolated from a leaf of the tree Platanus orientalis

Kang

Kim

Shin

et al. 2007

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

A novel bacterial strain, designated PMB02T, was isolated from a leaf of the tree Platanus orientalis. Colonies grown on TYG agar plates were circular, pink-pigmented and slow-growing, being 0.2–1.5 mm in diameter after 3 days growth. The cells of strain PMB02T were Gram-negative, aerobic, motile rods that possessed oxidase and catalase activities and grew at 20–30 °C, pH 6–8 and in media containing less than 1 % NaCl. The major respiratory quinone was identified as Q-10. A phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain PMB02T was related to members of the genus Methylobacterium. A comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Methylobacterium aquaticum and Methylobacterium variabile, with which it showed sequence similarities of 97.7 and 97.4 %, respectively. The values for DNA–DNA hybridization between strain PMB02T and M. aquaticum CCM 7218T and M. variabile GR3T were less than 32 %. On the basis of the phenotypic characterization, the phylogenetic analysis and the DNA–DNA relatedness data, strain PMB02T is considered to represent a novel species of the genus Methylobacterium, for which the name Methylobacterium platani sp. nov. is proposed. The type strain is PMB02T (=KCTC 12901T=JCM 14648T).

show abstract

Methylobacterium populi sp. nov., a novel aerobic, pink-pigmented, facultatively methylotrophic, methane-utilizing bacterium isolated from poplar trees (Populus deltoides×nigra DN34)

Cited by 222 publications

References 36 publications

Methylocella Species Are Facultatively Methanotrophic

Methylocella Species Are Facultatively Methanotrophic

Methylobacterium adhaesivum sp. nov., a methylotrophic bacterium isolated from drinking water

Methylobacterium platani sp. nov., isolated from a leaf of the tree Platanus orientalis

Contact Info

Product

Resources

About