Microbead-based immunoassay using the outer membrane layer of Escherichia coli combined with autodisplayed Z-domains

Kim, Do Hoon; Bong, Ji Hong; Yoo, Gu; Chang, Seo Yoon; Park, Min; Chang, Young Wook; Kang, Min Jung; Jose, Joachim; Pyun, Jae Chul

doi:10.1016/j.apsusc.2015.11.221

Cited by 7 publications

(1 citation statement)

References 21 publications

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“…In previous studies, a core-shell microsphere was synthesized using polystyrene as the core at first; the shell was then built using acrylamide as a template to capture adenosine, which was used as a bridge for conjugating antibody proteins [ 15 ]. Protein immobilization onto the microspheres can also be carried out through physical adsorption and electrostatic interactions, which was based on controlling of the surface charges by changing the percentage of styrene and covering the microspheres surface with bacterial outer membrane to increase the affinity of antibodies [ 16 ]. In addition, inorganic and metal materials were also considered as alternative choices due to their stable morphologies and special properties [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Rapid and Sensitive Detection of Cardiac Troponin I for Point-of-Care Tests Based on Red Fluorescent Microspheres

Cai

Kang²,

et al. 2018

Molecules

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A reliable lateral flow immunoassay (LFIA) based on a facile one-step synthesis of single microspheres in combining with immunochromatography technique was developed to establish a new point-of-care test (POCT) for the rapid and early detection of cardiac troponin I (cTnI), a kind of cardiac specific biomarker for acute myocardial infarction (AMI). The double layered microspheres with clear core-shell structures were produced using soap-free emulsion polymerization method with inexpensive compounds (styrene and acrylic acid). The synthetic process was simple, rapid and easy to control due to one-step synthesis without any complicated procedures. The microspheres are nanostructure with high surface area, which have numerous carboxyl groups on the out layer, resulting in high-efficiency coupling between the carrier and antibody via amide bond. Meanwhile, the red fluorescent dye, Nile-red (NR), was wrapped inside the microspheres to improve its stability, as well to reduce the background noise, because of its higher emission wavelength than interference from real plasma samples. The core-shell structures provided different functional areas to separate antibody and dyes, so the immunoassay has highly sensitive, wide working curves in the range of 0–40 ng/mL, low limits of detection (LOD) at 0.016 ng/mL, and limits of quantification (LOQ) at 0.087 ng/mL with coefficient of variations (CV) of 10%. This strategy suggested an outstanding platform for LFIA, with good reproducibility and stability to straightforwardly analyze the plasma samples without washing steps, thereby reducing the operating procedures for non-professionals and promoting detection efficiency. The whole detection process can be completed in less than 15 min. This novel immunoassay offers a reliable and favorable analytical result by detecting the real samples, indicating that it holds great potential as a new alternative for biomolecule detection in complex samples, for the early detection of cardiac specific biomarkers.

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Section: Introductionmentioning

confidence: 99%