Microbial biofilms in biotechnological processes

Maksimova, Yu. G.

doi:10.1134/s0003683814080043

Cited by 27 publications

(19 citation statements)

References 55 publications

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“…These include their preference for surface attachment, making them ideal for heterogeneous catalysis; and their protective self‐produced extracellular polymeric matrix, which can mitigate challenges related to toxicity, leading to long‐term productivity even under harsh conditions ,. Promising areas of application include biodegradation and biochemical synthesis …”

Section: Figurementioning

confidence: 99%

“…[8,9] Promising areas of application include biodegradation and biochemical synthesis. [10] Control over substrate concentration and reactor feeding strategy are the most important factors for optimization of whole-cell biocatalysis. [11,12] Chemostat bioreactors can impose tunable concentrations against the biofilm and eliminate nutrient depletion and product accumulation which occurs between solution replenishment cycles in bulk reactors.…”

Section: A Generalized Kinetic Framework Applied To Whole-cell Bioelementioning

confidence: 99%

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A Generalized Kinetic Framework Applied to Whole‐Cell Bioelectrocatalysis in Bioflow Reactors Clarifies Performance Enhancements for Geobacter Sulfurreducens Biofilms

et al. 2019

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A common kinetic framework for studies of whole‐cell catalysis is vital for understanding and optimizing bioflow reactors. In this work, we demonstrate the applicability of a flow‐adapted version of Michaelis‐Menten kinetics to an electrocatalytic bacterial biofilm. A three‐electrode microfluidic biofilm flow reactor measured increased turnover rates by as much as 50 % from a Geobacter sulfurreducens biofilm as flow rate was varied. Based on parameters from the applied kinetic framework, flow‐induced increases to turnover rate, catalytic efficiency and device reaction capacity could be linked to an increase in catalytic biomass. This study demonstrates that a standardized kinetic framework is critical for quantitative measurements of new living catalytic systems in flow reactors and for benchmarking against well‐studied catalytic systems such as enzymes.

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Section: Figurementioning

confidence: 99%

Section: A Generalized Kinetic Framework Applied To Whole-cell Bioelementioning

confidence: 99%

A Generalized Kinetic Framework Applied to Whole‐Cell Bioelectrocatalysis in Bioflow Reactors Clarifies Performance Enhancements for Geobacter Sulfurreducens Biofilms

et al. 2019

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“…One possible explanation is that rotating cultures provide a better nutrient and oxygen transport than static cultures, which is conducive to bacterial proliferation and metabolism (Serafica et al, 2002 ) and consequently enhances bacterial adverse-resistant activity. The other explanation is that the immobilization of BNC hydrogel on the fabric support is a natural form of cell immobilization, just like biofilm, which helps G. xylinus cells to achieve higher regional cell numbers and to resist the harmful circumstances (Cheng et al, 2009 ; Maksimova, 2014 ). Thus, compared with the static cultures, the bacteria were able to grow faster in the rotating cultures to achieve a higher cell density and to get a quick adaption to the CS-added cultures, which can be concluded from the growth curves (Figures 3c,C ).…”

Section: Resultsmentioning

confidence: 99%

Using In situ Dynamic Cultures to Rapidly Biofabricate Fabric-Reinforced Composites of Chitosan/Bacterial Nanocellulose for Antibacterial Wound Dressings

et al. 2016

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Bacterial nano-cellulose (BNC) is considered to possess incredible potential in biomedical applications due to its innate unrivaled nano-fibrillar structure and versatile properties. However, its use is largely restricted by inefficient production and by insufficient strength when it is in a highly swollen state. In this study, a fabric skeleton reinforced chitosan (CS)/BNC hydrogel with high mechanical reliability and antibacterial activity was fabricated by using an efficient dynamic culture that could reserve the nano-fibrillar structure. By adding CS in culture media to 0.25–0.75% (w/v) during bacterial cultivation, the CS/BNC composite hydrogel was biosynthesized in situ on a rotating drum composed of fabrics. With the proposed method, BNC biosynthesis became less sensitive to the adverse antibacterial effects of CS and the production time of the composite hydrogel with desirable thickness could be halved from 10 to 5 days as compared to the conventional static cultures. Although, its concentration was low in the medium, CS accounted for more than 38% of the CS/BNC dry weight. FE-SEM observation confirmed conservation of the nano-fibrillar networks and covering of CS on BNC. ATR-FTIR showed a decrease in the degree of intra-molecular hydrogen bonding and water absorption capacity was improved after compositing with CS. The fabric-reinforced CS/BNC composite exhibited bacteriostatic properties against Escherichia coli and Staphylococcus aureus and significantly improved mechanical properties as compared to the BNC sheets from static culture. In summary, the fabric-reinforced CS/BNC composite constitutes a desired candidate for advanced wound dressings. From another perspective, coating of BNC or CS/BNC could upgrade the conventional wound dressings made of cotton gauze to reduce pain during wound healing, especially for burn patients.

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“…These observations indicated the presence of an abundant extracellular polymeric matrix that enclosed the cells in the biofilm. In this context, microbial biofilms contain extracellular polymers, such as polysaccharides, polyuronic acids, proteins, nucleic acids and lipids, that allow adhesion to solid surfaces, as well as combination and stratification with other microorganisms that do not have the ability to form biofilms [31,49,50]. …”

Section: Resultsmentioning

confidence: 99%

Biodegradation of Methyl Tertiary Butyl Ether (MTBE) by a Microbial Consortium in a Continuous Up-Flow Packed-Bed Biofilm Reactor: Kinetic Study, Metabolite Identification and Toxicity Bioassays

et al. 2016

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This study investigated the aerobic biodegradation of methyl tertiary-butyl ether (MTBE) by a microbial consortium in a continuous up-flow packed-bed biofilm reactor using tezontle stone particles as a supporting material for the biofilm. Although MTBE is toxic for microbial communities, the microbial consortium used here was able to resist MTBE loading rates up to 128.3 mg L-1 h-1, with removal efficiencies of MTBE and chemical oxygen demand (COD) higher than 90%. A linear relationship was observed between the MTBE loading rate and the MTBE removal rate, as well as between the COD loading rate and the COD removal rate, within the interval of MTBE loading rates from 11.98 to 183.71 mg L-1 h-1. The metabolic intermediate tertiary butyl alcohol (TBA) was not detected in the effluent during all reactor runs, and the intermediate 2-hydroxy butyric acid (2-HIBA) was only detected at MTBE loading rates higher than 128.3 mg L-1 h-1. The results of toxicity bioassays with organisms from two different trophic levels revealed that the toxicity of the influent was significantly reduced after treatment in the packed-bed reactor. The packed-bed reactor system used in this study was highly effective for the continuous biodegradation of MTBE and is therefore a promising alternative for detoxifying MTBE-laden wastewater and groundwater.

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Microbial biofilms in biotechnological processes

Cited by 27 publications

References 55 publications

A Generalized Kinetic Framework Applied to Whole‐Cell Bioelectrocatalysis in Bioflow Reactors Clarifies Performance Enhancements for Geobacter Sulfurreducens Biofilms

A Generalized Kinetic Framework Applied to Whole‐Cell Bioelectrocatalysis in Bioflow Reactors Clarifies Performance Enhancements for Geobacter Sulfurreducens Biofilms

Using In situ Dynamic Cultures to Rapidly Biofabricate Fabric-Reinforced Composites of Chitosan/Bacterial Nanocellulose for Antibacterial Wound Dressings

Biodegradation of Methyl Tertiary Butyl Ether (MTBE) by a Microbial Consortium in a Continuous Up-Flow Packed-Bed Biofilm Reactor: Kinetic Study, Metabolite Identification and Toxicity Bioassays

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