Micronuclei assay of exfoliated oral buccal cells: Means to assess the nuclear abnormalities in different diseases

Kashyap, Bina; Reddy, P Sridhar

doi:10.4103/0973-1482.98968

Cited by 98 publications

(89 citation statements)

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“…6 Several studies have been conducted on micronuclei assay on the exfoliated cells of buccal mucosa of diabetic patients and have found that the frequency of micronuclei was increased in diabetic patients as compared to the normal individuals. Our results were consistent with the similar study conducted by Shashikala et al, 6 Kashyap and Reddy, 7 Holland et al, 8 which showed increased prevalence of micronuclei in diabetic patients as compared to the normal individuals. One of the parameters considered for the study was enlarged nuclei.…”

Section: Discussionsupporting

confidence: 93%

Cytomorphological Study of Oral Exfoliated Cells in Type II Diabetes Mellitus Patients

Sheethal¹,

Srinivasan²,

Priya³

et al. 2017

Journal of Health Sciences &Amp; Research

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Introduction: Diabetes mellitus (DM) is one of the most common endocrine metabolic disorders and its prevalence has been increasing worldwide. It is a third leading cause of mortality and morbidity in the world. Diabetes affects several organs including the oral cavity. The oral complications of uncontrolled DM can include xerostomia, infection, poor healing, increased incidence and severity of caries, candidiasis, gingivitis, periodontal disease, periapical abscesses, and burning mouth syndrome. Early diagnosis of the DM is an important aspect of health care. Exfoliative cytology is considered a moderate, straightforward, and noninvasive diagnostic marker for DM. The purpose of this study is to demonstrate cytologic changes of the exfoliated oral mucosal cells of diabetic patients using hematoxylin and eosin (H&E) staining technique to establish its role as diagnostic criteria.

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Section: Discussionsupporting

confidence: 93%

Cytomorphological Study of Oral Exfoliated Cells in Type II Diabetes Mellitus Patients

Sheethal¹,

Srinivasan²,

Priya³

et al. 2017

Journal of Health Sciences &Amp; Research

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“…The micronuclei were counted according to criteria given by Tolbert et al 12 The micronucleated cell frequency was calculated and the data obtained were subjected to statistical analysis. Only 1 out of 10 cases showed positivity with both Feulgen and Pap stain in control group, and in SCC, all cases showed positivity for micronuclei ( Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…The MN test is a noninvasive, cost-effective, and easily acceptable procedure by patients. [10][11][12] Based on this background, this study was undertaken to identify the feasible and economical method that could be used as a screening test in high-risk population for identifying the effects of genomic instabilities.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Micronuclei in Oral Squamous Cell Carcinoma: A Cytological Study

Chaudhary¹,

Ramesh²,

Oza³

et al. 2017

ijocrweb

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“…Embora os dados da literatura não demonstrem relação entre o sexo e a ocorrência dos parâmetros indicadores de genotoxicidade e citotoxicidade 28 , em nossas investigações, observamos maior frequência de brotamentos entre os homens do grupo controle.…”

Section: Grupo De Influênciaunclassified

Frequência de micronucleação e outras alterações nucleares em células da mucosa bucal de pacientes anêmicos

Tomaz

Ferri

Filho

2016

Rev. Fac. Ciênc. Méd. Sorocaba

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RESUMOIntrodução: O Teste Citogenético do Micronúcleo é um biomarcador que fornece informações para avaliar lesões cromossômicas oriundas de ações genotóxicas. A frequência de micronúcleos pode ser mensurada em situações de exposição a ambiente e substâncias com alto poder genotóxico. Muitas anemias apresentam sua gênese na deficiência nutricional e têm como consequência o aumento das lesões no DNA, assim como estresse oxidativo elevado. Ácido fólico e vitamina B12 determinam maior instabilidade cromossômica, seja pela carência de cofatores ou por lesões oxidativas no material genético, com implicações para o sistema de reparo do DNA. Objetivos: Considerando a correlação entre as anemias e os danos ao material genético, objetivou-se no presente estudo avaliar a frequência de micronucleação e de outras alterações nucleares em células da mucosa bucal de adultos anêmicos. Métodos: estudo conduzido com 60 indivíduos, dos quais 30 eram anêmicos e 30 compunham o grupo controle. As amostras foram colhidas e processadas conforme protocolo preestabelecido, e as lâminas confeccionadas foram avaliadas microscopicamente para os critérios de proliferação, de genotoxicidade, de citotoxicidade e de morte celular. Para análise estatística, os testes t de Student, de Levene, de correlação de Pearson, e teste de Shapiro-Wilk foram utilizados para a correlação das variáveis. Resultados: A frequência de micronucleação e de outras alterações nucleares foram maiores em anêmicos, enquanto o grupo controle apresentou maior capacidade proliferativa. Além disso, os hábitos de vida também influíram nos critérios celulares avaliados. Conclusões: A elevada frequência de micronúcleos em anêmicos foi atribuída ao estresse oxidativo, ao déficit nutricional ou a outras condições predisponentes, como a etiologia genética da anemia ou a presença de doença crônica. Palavras-chave: anemia; genotoxicidade; testes para micronúcleos. ABSTRACT Introduction:The Micronucleus Cytogenetic Test is a biomarker that provides information to assess chromosomal damage which arises from genotoxic actions. The micronucleus frequency can be measured at situations of substances and environmental exposure with high genotoxic power. Many anemias have their genesis in nutritional deficiencies and have the consequence of an increment in DNA lesions, altogether with high oxidative stress. Folic acid and B12 vitamin determines more chromosomal instability, such cofactors deficiency or oxidative lesions in genetic material, with implications in DNA repair system. Objectives: Considering the correlation of the anemias and damage to genetic material, the present study aims to evaluate the micronucleation rate and other nuclei alterations in oral mucosal cells of anemic adults. Methods: This study was conducted with 60 patients, 30 of them were anemic and 30 were in the control group. The samples were collected and processed according to a pre-established protocol and thereafter slides were microscopically evaluated for proliferation, genotoxicity, cytotoxicity and cell death...

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Micronuclei assay of exfoliated oral buccal cells: Means to assess the nuclear abnormalities in different diseases

Cited by 98 publications

References 0 publications

Cytomorphological Study of Oral Exfoliated Cells in Type II Diabetes Mellitus Patients

Cytomorphological Study of Oral Exfoliated Cells in Type II Diabetes Mellitus Patients

Evaluation of Micronuclei in Oral Squamous Cell Carcinoma: A Cytological Study

Frequência de micronucleação e outras alterações nucleares em células da mucosa bucal de pacientes anêmicos

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