2010
DOI: 10.1007/s11738-010-0680-x
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Micropropagation of Arnebia hispidissima (Lehm). DC. and production of alkannin from callus and cell suspension culture

Abstract: Alkannin, a red-purple dye and bioactive compound found in the roots of Arnebia hispidissima has antibiotic and anti-inflammatory properties and is also used in cosmetic and textile industries at a large-scale. In the present communication, we demonstrate the establishment of callus and cell suspension culture of A. hispidissima with the aim of optimizing the production of alkannin. Highest alkannin content was recorded in cell suspension and callus culture established on M-9 medium. Production of alkannin was… Show more

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Cited by 53 publications
(18 citation statements)
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“…The highest rooting frequency (100%) with maximum of (7.67±0.44) roots per shoot were achieved with 0.1 µM NAA (Figure 1c) where profuse rooting was observed. Similar results have also been achieved in Arnebia hispidissima (Shekhawat and Shekhawat, 2011) and regenerated plantlets were transferred to thermacoal cups consisted of sterilized garden soil and compost (1:1) as potting medium and maintained under controlled condition for two months prior to transfer to earthen pots containing garden soil the regenerated plant showed homogeneity with no phenotypic changes (Figure 1d). To confirm the genetic fidelity of the regenerated plants maintained in culture period of 6 months, RAPD analysis was carried out.…”
Section: Multiple Shoot Inductionsupporting
confidence: 80%
“…The highest rooting frequency (100%) with maximum of (7.67±0.44) roots per shoot were achieved with 0.1 µM NAA (Figure 1c) where profuse rooting was observed. Similar results have also been achieved in Arnebia hispidissima (Shekhawat and Shekhawat, 2011) and regenerated plantlets were transferred to thermacoal cups consisted of sterilized garden soil and compost (1:1) as potting medium and maintained under controlled condition for two months prior to transfer to earthen pots containing garden soil the regenerated plant showed homogeneity with no phenotypic changes (Figure 1d). To confirm the genetic fidelity of the regenerated plants maintained in culture period of 6 months, RAPD analysis was carried out.…”
Section: Multiple Shoot Inductionsupporting
confidence: 80%
“…1b). The increase in shoot number due to repeated transfer of mother explants may be owing to suppression of apical dominance during subculture which induced basal dominant meristematic cells to form new shoots (Tripathi and Kumari 2010;Shekhawat and Shekhawat 2011). This approach has also been reported in the micropropagation of a number of woody trees (Tripathi and Kumari 2010;Phulwaria et al 2011).…”
Section: Repeated Transfer Of the Mother Explantmentioning
confidence: 90%
“…The maximum response was observed during the third subculture. Repeated transfer of the mother explants help in increasing the shoot number may be because of suppression of apical dominance during subculture that induced basal meristematic cells to form new shoots (Shekhawat and Shekhawat, 2011). In another approach of shoot multiplication, amplified shoots were achieved by subculturing of in vitro produced shoots (separated from mother explant) on fresh culture medium.…”
Section: Multiplication Of Shootsmentioning
confidence: 99%