Micropropagation of Fennel (Foeniculum vulgare Miller)

Hunault, G.; Manoir, J. Du

doi:10.1007/978-3-662-07770-2_12

Cited by 9 publications

(2 citation statements)

References 23 publications

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“…Significant variability of morphological characters, biomass production and hypericin formation have been reported in regenerants of Hypericum perforatum originating from the same genotype (Èel-lárová et al, 1994). Hunault and du Manoir (1992) found differences in morphology, essential oil composition and fruit production in somatic embryoderived clones of Foeniculum vulgare. Saxena et al (2000) obtained two somaclones of Pelargonium graveolens from nodal explant-derived calluses.…”

Section: Plant Growth Characteristicsmentioning

confidence: 89%

Morphology, Secoiridoid Content and RAPD Analysis of Plants Regenerated from Callus of Centaurium erythraea RAFN

Piątczak

Królicka²,

Wysokińska³

2011

Acta Biologica Cracoviensia Series Botanica

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Centaurium erythraea plants obtained by indirect organogenesis are described in the paper. The plants were initiated from a single adventitious shoot regenerated from callus derived from the cotyledon of a 30-day-old seedling. The shoot was multiplied on MS medium supplemented with IAA (0.1 mg·L -1 ) and BAP (1.0 mg·L -1 ). The multiplication rate (28 shoots per culture within 4 weeks) was highest at the first subculture and decreased in further subcultures. The shoots were rooted on MS medium. The effect of IBA (0.1 mg·L -1 ) on the number of shoots forming roots differed depending on the composition of the basal medium (MS). The rooted shoots were transplanted to soil and grown in a greenhouse with 90% effectiveness. RAPD analysis was done with adventitious shoots of C. erythraea from in vitro culture. In shoots and whole plants regenerated from the callus tissue, secoiridoid content was determined by the HPLC method. We showed significant differences in morphology (leaf size, fresh and dry weight and height of plants) and changes in the DNA profiles as compared to earlier reports for shoot tip-derived shoots and plants of C. erythraea, but the two groups of plants biosynthesized the same qualitative pattern and similar levels of secoiridoids, up to 150 mg·g -1 dry weight; the increased biomass of plants regenerated from callus tissue makes them a better source of secondary metabolites.K Ke ey y w wo or rd ds s: : Centaurium erythraea Rafn., somaclonal variation, shoot culture, secoiridoid glycosides, indirect organogenesis.

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Section: Plant Growth Characteristicsmentioning

confidence: 89%

Morphology, Secoiridoid Content and RAPD Analysis of Plants Regenerated from Callus of Centaurium erythraea RAFN

Piątczak

Królicka²,

Wysokińska³

2011

Acta Biologica Cracoviensia Series Botanica

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“…Further, it also considered as a beneficial tool in obtaining fast growing organs with extensive branching and capability of producing the main metabolites of the mother plant or even new metabolites that neither detected in the mother plant nor in other kinds of in vitro cultures (Nader et al, 2006). So far, in vitro studies conducted on the production of the fennel secondary metabolites are mainly based on the callus or cell suspension cultures (Paupardin, 1976;Garcia-Rodriguez et al, 1978). However, plant cell suspension cultures for the production of secondary metabolites have been hampered by several limitations such as low yields of desired compounds, expensive culturing process, application of phytohormones, heterogeneous cell types, lack of storage tissue and products easily degraded by the enzymes released in the media (Davies & Deroles, 2014).…”

Section: Introductionmentioning

confidence: 99%

Effect of Agrobacterium rhizogenesis on hairy roots induction in fennel (Foeniculum vulgare Miller)

Abdi¹,

Uliaie²,

Bandehagh³

et al. 2017

JEBAS

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This study was conducted to access the effects of seven Agrobacterium rhizogenes strains i.e. A4, T4, MSU, TCC15834, 2656, Gm1 and R1000, on the hairy root generation from various explants i.e. hypocotyl, cotyledon, root, stem and leaf of Fennel ecotypes (Bonab, Izmir, Esfahan and Moghan). Results of study revealed that induction of hairy root varies with the A. rhizogenes strains and used explants. Among the various tested combinations, highest root induction was recorded form hypocotyl explants using A4 and TCC 15834 (70 and 67%, respectively) strains of A. rhizogenes. Response of explant toward hairy root induction and number of the hairy roots per explant was different and decreased from hypocotyl, leaf and cotyledonary explants, respectively. Among the various tested A. rhizogenes strain, TCC 15834 capable to induced hairy Roots development in all ecotypes but most effectively in Izmir (36.66 ± 0.57) and it was followed by Bonab (32 ± 0.44), Esfahan (30 ± 0.99) and Moghan (16.66 ± 0.79). All transformed hairy root were successfully confirmed by PCR using rolB primers. Further hairy root induction could be proved as an alternative approach for the production of secondary metabolites and its increasing use as model system in metabolic engineering. KEYWORDS Fennel Agrobacterium rhizogenesHairy root culture Hairy root induction Explant type

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Genetic stability and uniformity of Foeniculum vulgare Mill. regenerated plants through organogenesis and somatic embryogenesis

2004

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Micropropagation of Fennel (Foeniculum vulgare Miller)

Cited by 9 publications

References 23 publications

Morphology, Secoiridoid Content and RAPD Analysis of Plants Regenerated from Callus of Centaurium erythraea RAFN

Morphology, Secoiridoid Content and RAPD Analysis of Plants Regenerated from Callus of Centaurium erythraea RAFN

Effect of Agrobacterium rhizogenesis on hairy roots induction in fennel (Foeniculum vulgare Miller)

Genetic stability and uniformity of Foeniculum vulgare Mill. regenerated plants through organogenesis and somatic embryogenesis

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