Micropropagation of Stevia rebaudiana in temporary immersion systems as an alternative horticultural production method

Rosales, Catalina; Brenes, Jaime; Salas, Karla Melissa; Arce-Solano, Silvia; Abdelnour-Esquivel, Ana

doi:10.5154/r.rchsh.2017.08.028

Cited by 22 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The efficiency of the SETIS™ in comparison with other in vitro culture systems has been documented in stevia ( Stevia rebaudiana ) [ 22 ], where it doubled the number of shoots compared to the semi-solid culture system. In banana ( Musa AAA), Bello-Bello et al [ 11 ] achieved a three-fold increase in the number of shoots in the SETIS TM bioreactor compared to the semi-solid system.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Mancilla-Álvarez

Pérez-Sato

Núñez-Pastrana

et al. 2021

Plants

View full text Add to dashboard Cite

Taro is important for its nutritional content, medicinal use, and bioethanol production. The aim of the present study was to compare different semi-automated bioreactors (SABs) during in vitro multiplication of C. esculenta. The SABs used were temporary immersion bioreactors (TIBs), SETIS™ bioreactors and ebb-and-flow bioreactors; semi-solid culture medium was used as a control treatment. At 30 d of culture, different developmental variables, determination of chlorophyll, stomatal content, and survival percentage during acclimatization were evaluated. SABs increased the shoot multiplication rate relative to the semi-solid medium; however, the SETIS™ bioreactor showed the highest shoot production, with 36 shoots per explant, and the highest chlorophyll content. The stomatal index was higher in the semi-solid medium compared to the SABs, while the percentage of closed stomata was higher in the SABs than in the semi-solid culture medium. The survival rate during acclimatization showed no differences among the culture systems assessed, obtaining survival rates higher than 99%. In conclusion, the SETIS™ bioreactor showed the highest multiplication rate; however, other bioreactor alternatives are available for semi-automation and cost reduction for micropropagation of C. esculenta.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Mancilla-Álvarez

Pérez-Sato

Núñez-Pastrana

et al. 2021

Plants

View full text Add to dashboard Cite

show abstract

“…Para el contenido de micronutrientes en el tejido vegetal, el mayor contenido de B encontrado por el sistema IP puede ser explicado debido a que la absorción de este microelemento ocurre mediante difusión del espacio libre (Rosales et al, 2018). Características como el número de brotes, número de hojas y número de raíces, están relacionadas con la absorción de macro y micronutrientes, que fue superior en el biorreactor RITA ® .…”

Section: Discussionunclassified

In vitro nutrition of anthurium (Anthurium andreanum Linden var. Tropical) in three culture systems

López-Páez¹

2022

Mexjbiotechnol

View full text Add to dashboard Cite

Anthurium is a very important ornamental plant in the market. However, its traditional reproduction presents problems, therefore in vitro culture represents an alternative for its propagation. In the in vitro plant tissue culture, the nutrients are provided by the media, but information on the in vitro nutrition for anthurium is scarce. The objective of this research was to evaluate plant growth characteristics, nutrient content in plant tissues and nutrient removal from the culture medium in three in vitro culture systems: semi-solid, partial immersion and the RITA® bioreactor. The data were analyzed, using an ANOVA and compared with Tukey tests, using the statistical software R-STUDIO. The highest length of shoots and roots were observed with partial immersion; the number of leaves, shoots and roots did not show differences with the RITA® bioreactors. The highest tissue content of nutrients occurred in plants cultivated in the RITA® bioreactor. For the content of nutrients in the culture medium, the highest one was observed in the RITA® bioreactor, which indicates a most efficient use of nutrients, due to the greater contact of explants with the media and a best gas exchange. Therefore, the RITA® bioreactor represents the most viable option for anthurium plants production.

show abstract

“…A novel approach was developed using bacterium Bacillus safensis STJP extracted from rhizospheric soil of stevia for the formation of Paneer-whey based bio-formulation which increased the fresh, dry weight and stevioside content through nutrient(s) linked mechanism [129]. RITA, BIT and SETIS temporary immersion systems were studied for scaling up micropropagation for biomass evaluation in the field using different concentrations of calcium pantothenate, sucrose and gibberellic acid and it was found that temporary immersion systems can boost plant production [130].…”

Section: Studies To Overcome Production Constraintsmentioning

confidence: 99%

Genetic Improvement of Stevia: A Natural Non-Calorie Sweetener

Gautam¹,

Kumar²,

Kashyap³

et al. 2023

Case Studies of Breeding Strategies in Major Plant Species

View full text Add to dashboard Cite

Stevia rebaudiana, a native of South America, is a perennial herb of the Asteraceae family, also known as a natural sweetener due to the presence of steviol glycosides (SGs) in the leaves. China is the largest producer and exporter of stevia, while Japan is the primary consumer. The increasing demand for natural low-calorie sweeteners in the medicine and food industry has increased the pressure over stevia cultivation. Still, its cultivation and region-specific agrotechnologies need to be developed. The major bottleneck in stevia production are the lack of region specific cultivation technologies, non-availability of quality planting material, and uncharacterized and not properly conserved plant genetic resources. All these constraints have limited the stevia production to some specific regions of the world. Development of high-yielding cultivars with enhanced SGs content using modern breeding techniques is of prime importance to meet its increasing demand. Among the glycosides present in the leaves, rebaudioside-A is the most desirable glycosides having 250–300 times sweeter than sucrose, while, after bitter taste is due to the presence of stevioside and dulcoside. Therefore, the development of varieties with high rebaudioside-A and low stevioside content is highly desirable. This chapter focused on the improvement of propagation methods, characterization and conservation of genetic resource in stevia and its utilization in crop improvement programs.

show abstract

Micropropagation of Stevia rebaudiana in temporary immersion systems as an alternative horticultural production method

Cited by 22 publications

References 0 publications

Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

In vitro nutrition of anthurium (Anthurium andreanum Linden var. Tropical) in three culture systems

Genetic Improvement of Stevia: A Natural Non-Calorie Sweetener

Contact Info

Product

Resources

About