Migration of phagocytotic cells and development of the murine intraretinal microglial network: An in vivo study using fluorescent dyes

Bodeutsch, Nicole; Thanos, Solon

doi:10.1002/1098-1136(200010)32:1<91::aid-glia90>3.0.co;2-x

Cited by 60 publications

(48 citation statements)

References 47 publications

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“…Morphological features and distribution of cathepsin B/D-expressing cells are similar to those previously described for macrophages and microglial precursors during vertebrate embryonic development (Bodeutsch and Thanos, 2000;Rodriguez-Gallardo et al, 2005;Nishitani and Sasaki, 2006;Santos et al, 2008). The definitive characterization of cathepsin B/ D-expressing cells was confirmed by their histochemical labeling with TL that recognizes macrophage/microglial cells in the nervous system of rodents (Acarin et al, 1994;Santos et al, 2008).…”

Section: Cathepsin B and D Expression In Macrophages And Microglial Psupporting

confidence: 76%

“…The morphological features and spatiotemporal distribution of cathepsin B/D-expressing cells are highly coincident with those described for macrophages and microglial precursors within the developing and postnatal mouse retina (Bodeutsch and Thanos, 2000;Rodriguez-Gallardo et al, 2005;Santos et al, 2008). We have combined cathepsin D expression with inmunohistochemystry of retinal cellspecific markers to detect the nature of these cells.…”

Section: Characterization Of the Cathepsin B/d-expressing Cellsmentioning

confidence: 62%

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Macrophage and microglia ontogeny in the mouse visual system can be traced by the expression of Cathepsins B and D

Bejarano-Escobar

Holguín-Arévalo

Montero

et al. 2011

Developmental Dynamics

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Here, we show a detailed chronotopographical analysis of cathepsin B and D expression during development of the mouse visual system. Both proteases were detected in large rounded/ameboid cells usually located in close relationship with prominent sites of extensive physiological cell death. In concordance with their morphological features and topographical distribution, we demonstrate that expressing cells corresponded with macrophages and microglial precursors. We found that as microglial precursors differentiated the expression of both cathepsins was down-regulated. Of interest, cathepsin B and D transcripts were never observed in degenerating cells. Our findings point to a role for cathepsin D and B in cell debris degradation after apoptotic processes rather than promoting cell death, as proposed for other developmental models. Additionally their pattern of expression suggests a role in the maturation of the microglial precursors.

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Section: Cathepsin B and D Expression In Macrophages And Microglial Psupporting

confidence: 76%

Section: Characterization Of the Cathepsin B/d-expressing Cellsmentioning

confidence: 62%

Macrophage and microglia ontogeny in the mouse visual system can be traced by the expression of Cathepsins B and D

Bejarano-Escobar

Holguín-Arévalo

Montero

et al. 2011

Developmental Dynamics

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“…From E9 to the first half of the first posthatching week, microglial cells migrating on the vitreal surface of the retina can move radially in a vitreal-to-scleral direction to gain access to the inner plexiform layer (IPL) and the outer plexiform layer (OPL), where they become ramified microglia (Navascués et al, 1995;Marín-Teva et al, 1999c). Observations by various studies appear to support the view that tangential (Diaz-Araya et al, 1995aProvis et al, 1996) and radial (Hume et al, 1983;Bodeutsch and Thanos, 2000) migration of amoeboid microglia also occurs in the retina of mammals.…”

Section: Introductionmentioning

confidence: 87%

Radial migration of developing microglial cells in quail retina: A confocal microscopy study

Sánchez‐López

Cuadros

Calvente

et al. 2004

Glia

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Microglial cells spread within the nervous system by tangential and radial migration. The cellular mechanism of tangential migration of microglia has been described in the quail retina but the mechanism of their radial migration has not been studied. In this work, we clarify some aspects of this mechanism by analyzing morphological features of microglial cells at different steps of their radial migration in the quail retina. Microglial cells migrate in the vitreal half of the retina by successive jumps from the vitreal border to progressively more scleral levels located at the vitreal border, intermediate regions, and scleral border of the inner plexiform layer (IPL). The cellular mechanism used for each jump consists of the emission of a leading thin radial process that ramifies at a more scleral level before retraction of the rear of the cell. Hence, radial migration and ramification of microglial cells are simultaneous events. Once at the scleral border of the IPL, microglial cells migrate through the inner nuclear layer to the outer plexiform layer by another mechanism: they retract cell processes, become round, and squeeze through neuronal bodies. Microglial cells use radial processes of s-lamininexpressing Mü ller cells as substratum for radial migration. Levels where microglial cells stop and ramify at each jump are always interfaces between retinal strata with strong tenascin immunostaining and strata showing weak or no tenascin immunoreactivity. When microglial cell radial migration ends, tenascin immunostaining is no longer present in the retina. These findings suggest that tenascin plays a role in the stopping and ramification of radially migrating microglial cells.

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“…Phagocytic murine retinal microglia have been traced by retro-grade labeling of ganglion cells with different dyes 17 , but more common now is in vivo imaging with GFP-expressing transgenic animals [18][19][20][21] and/or immunostaining with the marker ionized calcium binding adapter molecule 1 (Iba1) 22 . The retinal fundus is optimal for non-invasive imaging and GFP-labeled or autofluorescent phagocytes can be visualized with a confocal scanning laser ophthalmoscope in the blue auto-fluorescence mode.…”

Section: Development Of the Protocolmentioning

confidence: 99%

Comprehensive analysis of mouse retinal mononuclear phagocytes

et al. 2017

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Migration of phagocytotic cells and development of the murine intraretinal microglial network: An in vivo study using fluorescent dyes

Cited by 60 publications

References 47 publications

Macrophage and microglia ontogeny in the mouse visual system can be traced by the expression of Cathepsins B and D

Macrophage and microglia ontogeny in the mouse visual system can be traced by the expression of Cathepsins B and D

Radial migration of developing microglial cells in quail retina: A confocal microscopy study

Comprehensive analysis of mouse retinal mononuclear phagocytes

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