Mineral nutrition of<i>Gypsophila in vitro</i>root culture

Morard, P.; Fulcheri, Corinne; Henry, Max

doi:10.1080/01904169909365667

Cited by 4 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In these microplants, some kind of antagonism can be observed between K + absorption and NH 4 + resulting from NH 4 + charge accumulation inside the cells (Morard et al 1999). About 80-90% of the initial PO 4 3− was absorbed under all assayed conditions, and this is in agreement with the results obtained in other studies (Leifert et al 1991;Barbas et al 1993;Morard et al 1998;Moncaleán et al 2003).…”

Section: Discussionsupporting

confidence: 90%

CO2-enriched microenvironment affects sucrose and macronutrients absorption and promotes autotrophy in the in vitro culture of kiwi (Actinidia deliciosa Chev. Liang and Ferguson)

Arigita

Cañal

Tamés

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

In traditional in vitro culture, the low CO 2 concentration inside the vessels restricts photosynthesis and necessitates the addition of sucrose to the culture medium as the main energy source, thus bringing about changes in the absorption of mineral elements from the culture medium. In this study, we investigated macronutrient absorption and sugar consumption in Actinidia deliciosa Chevalier Liang and Ferguson cv. Hayward (kiwi), cultured on medium supplemented with varying amounts of sucrose (0, 10, and 20 g l −1 ) under both heterotrophy and autotrophy, flushed with different concentrations of CO 2 (non-ventilation, 300, 600, and 2,000 μl l −1 ). In ventilated systems with 20 g l −1 of sucrose, sucrose absorption was less than under non-ventilation. The lowest rate of sucrose absorption was recorded when the explants were cultured on medium supplemented with 20 g l −1 of sucrose and flushed with 600 μl l −1 CO 2 . Absorption of NO 3 − , PO 4 3− , and Mg 2+ were high (maximum) at the end of the culture period (40 d) in explants flushed with 600 μl l −1 CO 2 that have been cultured 20 d in the presence of sucrose and then transferred to a sucrose-free medium. These autotrophic conditions promoted maximum plant growth in terms of both fresh and dry mass as well as the length and number of shoots and leaves. The study shows that to maintain an optimum regime of mineral nutrition for prolonged culture of kiwi in vitro, an increased amount of these three ions should be supplemented in Murashige and Skoog's medium.

show abstract

Section: Discussionsupporting

confidence: 90%

CO2-enriched microenvironment affects sucrose and macronutrients absorption and promotes autotrophy in the in vitro culture of kiwi (Actinidia deliciosa Chev. Liang and Ferguson)

Arigita

Cañal

Tamés

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

show abstract

“…After 12 months of culture on solid MH3 media supplemented with 2,4-Dichlorophenoxyacetic acid [2.5 µM] 1-Naphthaleneacetic acid [0.5 µM] Kinetin [1.0 µM] and callus fragments (0.5 g) were dispersed by vortexing to contain mostly individual cells and suspended in 100 mL of liquid MH3 phytohormone-free medium. MH3 medium was prepared according to Morard et al 1999 [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

Bacterial Nanocellulose Fortified with Antimicrobial and Anti-Inflammatory Natural Products from Chelidonium majus Plant Cell Cultures

et al. 2021

View full text Add to dashboard Cite

In this work we developed a bi-functional Bacterial-Nano-Cellulose (BNC) carrier system for cell cultures of Chelidonium majus—a medicinal plant producing antimicrobial compounds. The porous BNC was biosynthesized for 3, 5 or 7 days by the non-pathogenic Komagataeibacter xylinus bacteria and used in three forms: (1) Without removal of K. xylinus cells, (2) partially cleaned up from the remaining K. xylinus cells using water washing and (3) fully purified with NaOH leaving no bacterial cells remains. The suspended C. majus cells were inoculated on the BNC pieces in liquid medium and the functionalized BNC was harvested and subjected to scanning electron microscopy observation and analyzed for the content of C. majus metabolites as well as to antimicrobial assays and tested for potential proinflammatory irritating activity in human neutrophils. The highest content and the most complex composition of pharmacologically active substances was found in 3-day-old, unpurified BNC, which was tested for its bioactivity. The assays based on the IL-1β, IL-8 and TNF-α secretion in an in vitro model showed an anti-inflammatory effect of this particular biomatrix. Moreover, 3-day-old-BNC displayed antimicrobial and antibiofilm activity against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. The results of the research indicated a possible application of such modified composites, against microbial pathogens, especially in local surface infections, where plant metabolite-enriched BNC may be used as the occlusive dressing.

show abstract

Micropropagation of dahlia in static liquid medium using slow-release tools of medium ingredients

Klerk¹,

Brugge²

2011

Scientia Horticulturae

View full text Add to dashboard Cite

Mineral nutrition ofGypsophila in vitroroot culture

Cited by 4 publications

References 15 publications

CO2-enriched microenvironment affects sucrose and macronutrients absorption and promotes autotrophy in the in vitro culture of kiwi (Actinidia deliciosa Chev. Liang and Ferguson)

CO2-enriched microenvironment affects sucrose and macronutrients absorption and promotes autotrophy in the in vitro culture of kiwi (Actinidia deliciosa Chev. Liang and Ferguson)

Bacterial Nanocellulose Fortified with Antimicrobial and Anti-Inflammatory Natural Products from Chelidonium majus Plant Cell Cultures

Micropropagation of dahlia in static liquid medium using slow-release tools of medium ingredients

Contact Info

Product

Resources

About