MiR-34a and miR-203 Inhibit Survivin Expression to Control Cell Proliferation and Survival in Human Osteosarcoma Cells

Chen, Xun; Chen, Xiaogang; Hu, Xiaojing; Song, Tao; Ou, Xuehai; Zhang, Caiguo; Zhang, Wentao; Zhang, Chun

doi:10.7150/jca.15061

Cited by 43 publications

(33 citation statements)

References 25 publications

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“…According to previous reports [10][11][12], 2588 mature miRNAs have been identified in the human body, and each miRNA can widely participate in various life activities of the human body, and regulate the occurrence and development of diseases and cancers. A number of studies [13,14] showed that miR-203, miR-148b and other miRNAs were abnormally MiR-143 is located on human chromosome 5 and is downregulated in the bladder and ovarian cancer. Its expression level is significantly correlated with clinical stage, disease grade and lymph node metastasis.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of miR-143-3p inhibiting proliferation, migration and invasion of osteosarcoma cells by targeting MAPK7

Hou

Feng

Jiao

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Objective: To investigate the effects of miR-143-3p and MAPK7 on the proliferation, migration, and invasion of U2OS human osteosarcoma (OS) cells. Methods: The expression of miR-143-3p and MAPK7 in U2OS cells were detected by qRT-PCR, and the protein level of MAPK7 was measured by western blot assay. The targeting relationship between miR-143-3p and MAPK7 was predicted by TargetScan and verified by dual luciferase reporter assay. MTT and Transwell assays were used to detect cell viability, migrated cells and invaded cells of U2OS cells. Results: Compared with hFOB1.19 cells, the expression of miR-143-3p was down-regulated and MAPK7 was up-regulated in U2OS cells. Cell viability, migration and invasion ability significantly decreased induced by miR-143-3p overexpression or MAPK7 knockdown in U2OS cells. The results of dual luciferase reporter assay indicated that miR-143-3p interacted with MAPK7. Furthermore, overexpression of MAPK7 could reverse the inhibitory effects on cell proliferation, migration and invasion in U2OS cells induced by miR-143-3p mimics. Conclusion: miR-143-3p could inhibit proliferation, migration and invasion of U2OS cells by targeting MAPK7.

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Section: Discussionmentioning

confidence: 99%

Mechanism of miR-143-3p inhibiting proliferation, migration and invasion of osteosarcoma cells by targeting MAPK7

Hou

Feng

Jiao

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…Also, in the field of osteosarcoma research, there are much more recent research confirmed that mir-34a can inhibit cell proliferation and promotes apoptosis in human osteosarcoma cells in vitro and in vivo. [21][22][23][24][25][26][27] So, we are valid to consider that mir-34a could inhibit cell proliferation and promotes apoptosis in osteosarcoma possibly. And our results strongly suggest that miR-34a was downregulated in osteosarcoma cells.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-34a inhibits tumor invasion and metastasis in osteosarcoma partly by effecting C-IAP2 and Bcl-2

et al. 2017

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Osteosarcoma is a common primary malignant bone tumor that occurs mainly in children and adolescents. Recent evidence has demonstrated that miR-34a is involved in the invasion and metastasis of osteosarcoma. This study aims to explore the effect of biological behavior of miR-34a on osteosarcoma. First, we collect osteosarcoma and adjacent specimens, and the relative expression of miR-34a and C-IAP2 messenger RNA was quantitated by real-time polymerase chain reaction. Furthermore, miR-34a stimulant is synthesized and transfected onto osteosarcoma MG-63 cells. The effect of overexpression of miR-34a on osteosarcoma was detected by colony-forming assay, Annexin V-fluorescein isothiocyanate Apoptosis Detection Kit I, Transwell assay, and animal experiment in vivo. Finally, the relative levels of C-IAP2 and Bcl-2 protein were checked by western blot, and the activity of caspase-3 and caspase-9 was tested by spectrophotometry assay. In conclusion, miR-34a was downregulated in osteosarcoma cells. And the expression of C-IAP2 and Bcl-2 protein was drastically inhibited, and the activities of caspase-3 and caspase-9 were significantly increased after transfecting miR-34a onto osteosarcoma MG-63 cells. And the overexpression of miR-34a can inhibit cell invasion and metastasis, promote cell apoptosis, and arrest cells in G0/G1 period. And the animal experiment in vivo demonstrated that the overexpression of miR-34a could significantly inhibit the growth of osteosarcoma in animal skin. Taken together, we indicated that miR-34a can inhibit tumor invasion and metastasis in osteosarcoma, and its mechanism may be partly related to downregulating the expression of C-IAP2 and Bcl-2 protein directly or indirectly.

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“…Naloxone can reverse this morphineinduced upregulation, proving that opioid receptors were involved in the process. Recently, it has been found that over-expressing Survivin can promote tumors to metastasize and increase genomic instability, thereby promoting tumor invasion and metastasis [39,40]. It was also shown that morphine can enhance RCC growth by promoting Survivin expression [39,40].…”

Section: Effect Of Morphine On Tumor Invasion and Metastasismentioning

confidence: 99%

The dual effect of morphine on tumor development

Tuerxun

Cui

2018

Clin Transl Oncol

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Morphine is a classic opioid drug used for reducing pain and is commonly prescribed as an effective drug to control cancer pain. Morphine has a direct role in the central nervous system to relieve pain, but because of its peripheral functions, morphine also has some side effects, such as nausea, constipation, and addiction (Gupta et al. in Sci World J 2015:10, 2015). In addition to its analgesic effect, the role of morphine in tumor development is an important question that has been investigated for many years with conflicting results. Numerous studies suggest that morphine has a role in both promoting and inhibiting tumor growth. In this extensive review, we attempt to comprehensively understand the effects of morphine and summarize both its positive and negative influences on various aspects of tumors, including tumor growth, angiogenesis, metastasis, inflammation, and immunomodulation.

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MiR-34a and miR-203 Inhibit Survivin Expression to Control Cell Proliferation and Survival in Human Osteosarcoma Cells

Cited by 43 publications

References 25 publications

Mechanism of miR-143-3p inhibiting proliferation, migration and invasion of osteosarcoma cells by targeting MAPK7

Mechanism of miR-143-3p inhibiting proliferation, migration and invasion of osteosarcoma cells by targeting MAPK7

MicroRNA-34a inhibits tumor invasion and metastasis in osteosarcoma partly by effecting C-IAP2 and Bcl-2

The dual effect of morphine on tumor development

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