miRNA-186 inhibits prostate cancer cell proliferation and tumor growth by targeting YY1 and CDK6

Lu, Shu; Wang, Mingshan; Chen, Peijie; Ren, Qiang; Bai, Péter

doi:10.3892/etm.2017.4387

Cited by 39 publications

(34 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After cDNA synthesis using the reverse transcription kit (Promega, Madison, WI), the expression level of YY1 mRNA was quantified by RT‐PCR with Power SYBR Green PCR Master Mix (Carlsbad, CA) and GAPDH as a control for normalization. The sequence of YY1 primer was as follows: YY1, 5′‐CAACAAGAAGTGGGAGCA‐3′ and 3′‐CCACTGTCTCATGGTCAA‐5′ (Lu et al, ). The 2 −ΔΔ C t method was performed to calculate the relative expression of target genes.…”

Section: Methodsmentioning

confidence: 99%

Retracted: MiR‐141‐3p Suppresses Tumor Growth and Metastasis in Papillary Thyroid Cancer via Targeting Yin Yang 1

Fang

Huang

et al. 2018

The Anatomical Record

View full text Add to dashboard Cite

has been found to be downregulated in papillary thyroid carcinoma (PTC), while little is known about the cellular functions and precise signals elicited by miR-141-3p in PTC. The results of this study indicated that the expression of miR-141-3p was aberrantly down-regulated in PTC tissues and cell lines, compared with the adjacent normal tissues and normal thyroid epithelial cells. Furthermore, the miR-141-3p expression level was negatively associated with TNM stage and lymph node metastasis in PTC. Expression of miR-141-3p effectively inhibited cell growth, promoted apoptosis, and suppressed invasion in PTC cells. Meanwhile, miR-141-3p knockdown with miR-141-3p inhibitor reversed these effects. Consistent with the in vitro study, miR-141-3p also exhibited anti-neoplastic activity in vivo. Moreover, the results revealed that miR-141-3p directly recognized the 3 0 untranslated region (3 0 UTR) of Yin Yang 1 (YY1) and negatively regulated the expression of YY1 at both protein and mRNA levels. Ectopic expression of YY1 could effectively abrogate the anti-metastatic and proapoptotic effects of miR-141-3p. In summary, the findings suggested that miR-141-3p can act as a tumor suppressor in PTC and may be a potential therapeutic target for PTC treatment. Anat Rec, 302:258-268, 2019. -3p; YY1; tumor growth; metastasis 10%-15% of patients will relapse and develop distant metastases, leading to a poor clinical outcome (Sipos and Mazzaferri, 2010). Thus, further exploring the underlying mechanisms contributing to the occurrence, progression, and metastasis of PTC may provide insight into the novel target therapies. MicroRNAs (miRNAs) belong to a group of small noncoding RNA, and play a pivotal role in various physiological and pathological processes during cancer development

show abstract

Section: Methodsmentioning

confidence: 99%

Retracted: MiR‐141‐3p Suppresses Tumor Growth and Metastasis in Papillary Thyroid Cancer via Targeting Yin Yang 1

Fang

Huang

et al. 2018

The Anatomical Record

View full text Add to dashboard Cite

show abstract

“…Lu et al . found that miR‐186, which inhibits prostate cancer cell growth in vitro and tumors in nude mice, downregulates YY1 expression and that of cyclin‐dependent kinase 6 (cdk6) by binding to their 3′ UTRs …”

Section: Yy1 and Tumor Growthmentioning

confidence: 99%

The Yin and Yang of YY1 in tumor growth and suppression

Khachigian

2018

Intl Journal of Cancer

132

106

View full text Add to dashboard Cite

Yin Yang-1 (YY1) is a zinc finger protein and member of the GLI-Kruppel family that can activate or inactivate gene expression depending on interacting partners, promoter context and chromatin structure, and may be involved in the transcriptional control of ∼10% of the total mammalian gene set. A growing body of literature indicates that YY1 is overexpressed in multiple cancer types and that increased YY1 levels correlate with poor clinical outcomes in many cancers. However, the role of YY1 in the promotion or suppression of tumor growth remains controversial and its regulatory effects may be tumor cell type dependent at least in experimental systems. The molecular mechanisms responsible for the apparently conflicting roles of YY1 are not yet fully elucidated. This review highlights recent advances in our understanding of regulatory insights involving YY1 function in a range of cancer types. For example, YY1's roles in tumor growth involve stabilization of hypoxia-inducible factor HIF-1α in a p53 independent manner, negative regulation of miR-9 transcription, control of MYCT1 transcription, a novel miR-193a-5p-YY1-APC axis, intracellular ROS and mitochondrial superoxide generation, p53 reduction and EGFR activation, control of genes associated with mitochondrial energy metabolism and miRNA regulatory networks involving miR-7, miR-9, miR-34a, miR-186, miR-381, miR-584-3p and miR-635. On the other hand, tumor suppressor roles of YY1 appear to involve YY1 stimulation of tumor suppressor BRCA1, increased Bax transcription and apoptosis involving cytochrome c release and caspase-3/-7 cleavage, induction of heme oxygenase-1, inhibition of pRb phosphorylation and p21 binding to cyclin D1 and cdk4, reduced expression of long noncoding RNA of SOX2 overlapping transcript, and MUC4/ErbB2/p38/MEF2C-dependent downregulation of MMP-10. YY1 expression is associated with that of cancer stem cell markers SOX2, BMI1 and OCT4 across many cancers suggesting multidynamic regulatory control and groups of cancers with distinct molecular signatures. Greater understanding of the mechanistic roles of YY1 will in turn lead to the development of more specific approaches to modulate YY1 expression and activity with therapeutic potential.

show abstract

“…miRNA is a non-coding RNA closely related to the development and progression of tumors, and its role in prostate cancer has been widely discussed in recent years (11). There are various miRNA targets that can be used for the diagnosis and treatment of prostate cancer, with more tumor suppressor genes and fewer oncogenes (12,13). A study has shown that the upregulation of miR-101 inhibits the proliferation and invasion of prostate cancer cell line Du145 by inhibiting its target gene EZH2 (14).…”

Section: Discussionmentioning

confidence: 99%

Effects of miR‑129‑3p on biological functions of prostate cancer cells through targeted regulation of Smad3

2019

View full text Add to dashboard Cite

Effects of miR-129-3p on the biological functions of prostate cancer cells through the targeted regulation of Smad3 were investigated. RT-PCR was used to detect the expression levels of miR-129-3p in prostate cancer tissues and cells and its target gene Smad3 mRNA determined by bioinformatics prediction. Correlation between miR-129-3p and Smad3 was analyzed. MTT assay, cell invasion detection, and apoptosis detection were conducted to detect the effects of miR-129-3p and Smad3 on the proliferation, invasion, and apoptosis of prostate cancer cells. The results of RT-qPCR showed that the expression level of miR-129-3p decreased but that of Smad3 increased in the prostate cancer tissue, and the expression levels of the two were significantly and negatively correlated. Additionally, the expression levels were closely related to the degree of tumor differentiation, TNM staging, and lymph node metastasis (P<0.05). Bioinformatics prediction and subsequent experiments proved that Smad3 was the direct target gene of miR-129-3p. Cell detection confirmed that the overexpression of miR-129-3p or the inhibition of Smad3 expression inhibited the proliferation and invasion of prostate cancer cells, promoting apoptosis, and increased the expression level of pro-apoptotic protein Bax, as well as decreased the expression level of anti-apoptotic protein Bcl-2. Inhibition of miR-129-3p expression had the opposite effect to overexpression. miR-129-3p, which may be a new and potential target for the treatment of prostate cancer, can inhibit the proliferation and invasion of prostate cancer cells and promote their apoptosis by directly targeting Smad3.

show abstract

miRNA-186 inhibits prostate cancer cell proliferation and tumor growth by targeting YY1 and CDK6

Cited by 39 publications

References 27 publications

Retracted: MiR‐141‐3p Suppresses Tumor Growth and Metastasis in Papillary Thyroid Cancer via Targeting Yin Yang 1

Retracted: MiR‐141‐3p Suppresses Tumor Growth and Metastasis in Papillary Thyroid Cancer via Targeting Yin Yang 1

The Yin and Yang of YY1 in tumor growth and suppression

Effects of miR‑129‑3p on biological functions of prostate cancer cells through targeted regulation of Smad3

Contact Info

Product

Resources

About