The level of submicroscopic representation can be cause of difficulty in learning chemistry, so that interest and understanding of chemistry concept is low. In overcoming this, innovative learning media are needed that are able to visualize and make it easier to understand abstract concepts, especially on the subtopic of the effect of reactant’s concentration and catalyst on the chemical rate reactions. This research aims to create product in the form of Augmented Reality assisted by Assemblr Edu that is valid, effective, and practical. This study is Research and Development that uses ADDIE development model and involves 10 prospective chemistry teacher students. Research instruments include validation sheets, test items, and response questionnaire. Data were analyzed using Aiken's V and descriptive statistic. The results showed that: (1) product have fulfilled very valid criteria with average percentage of 92% with aspect of product presentation and use are 92% (very valid), content aspect of 94% (very valid); language aspect by 92% (very valid); (2)average test score of respondents after using the product is 70 which indicates that product is classified as effective; and (3)average response to the product is 90% indicating that the product is very good and classified as very practical. The product is feasible to use and can support the mastery of technology-based concept, especially in the subtopic of the effect of reactant’s concentration and catalyst on chemical rate reactions.