Moderne Konzepte zur dynamischen Konservierung von Leber und Nieren im Rahmen einer Transplantation

Horn, Charlotte von; Minor, Thomas

doi:10.1007/s00292-019-0595-2

Cited by 7 publications

(4 citation statements)

References 59 publications

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“…32 This role enables the liver to participate in energy metabolism of various tissues and organs. 32 In the liver, insulin inhibits a complex metabolic process, gluconeogenesis, thereby promoting macromolecule glycogen synthesis and suppressing hepatic glucose production. 33 Hepatic IR can cause unrestrained gluconeogenesis, resulting in hyperglycemia.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…The liver is a place where glucose and lipid are metabolized most frequently, thus playing a vital role in regulating insulin, glucagon, GH, epinephrine, and other hormones 32 . This role enables the liver to participate in energy metabolism of various tissues and organs 32 . In the liver, insulin inhibits a complex metabolic process, gluconeogenesis, thereby promoting macromolecule glycogen synthesis and suppressing hepatic glucose production 33 .…”

Section: Discussionmentioning

confidence: 99%

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Astragalus polysaccharide ameliorates insulin resistance in HepG2 cells through activating the STAT5/IGF‐1 pathway

Yue,

Hao,

Wang

et al. 2023

Immunity Inflam & Disease

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BackgroundInsulin resistance (IR) is considered as a major factor initiating type 2 diabetes mellitus and can lead to a reduction in glucose uptake that mainly occurs in the liver. Astragalus polysaccharide (APC), extracted from the traditional Chinese medicine, has been recorded to suppress IR. However, the underlying mechanism remains inadequately explored.MethodsIR was induced in HepG2 cells which further underwent APC treatment. Cell viability was determined by cell counting kit‐8 assay. Pretreatment with AG490, an inhibitor of signal transducer and activator of transcription 5 (STAT5) signaling, was performed for investigating the influence of STAT5 on APC. Glucose uptake level was reflected by 2‐deoxyglucose‐6‐phosphate content determined through colorimetric assay. Expression levels of insulin‐like growth factor 1 (IGF‐1), IGF‐1 receptor (IGF‐1R), phosphorylated‐STAT5/STAT5, and p‐protein kinase B (AKT)/AKT in the cells were assessed by Western blot. Radioimmunoassay (RIA) was used to detect IGF‐1 secretion in the cells.ResultsAPC at doses of 10 and 20 mg increased the viability of HepG2 cells with/without IR induction, and abrogated IR‐induced inhibition of glucose intake. Meanwhile, APC (10 mg) offset IR‐induced inhibition on the expressions of IGF‐1R and IGF‐1, the activation of AKT and STAT5, and the secretion of IGF‐1 in HepG2 cells. More importantly, the reversal effect of APC on IR‐induced alterations in HepG2 cells was counteracted by AG490.ConclusionAPC ameliorates IR in HepG2 cells through activating the STAT5/IGF‐1 pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Astragalus polysaccharide ameliorates insulin resistance in HepG2 cells through activating the STAT5/IGF‐1 pathway

Yue,

Hao,

Wang

et al. 2023

Immunity Inflam & Disease

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show abstract

“…The liver is the main organ of glucose and lipid metabolism and plays an important role in energy metabolism of various tissues and organs including muscles and adipose tissues through regulation of insulin, glucagon, epinephrine, growth hormone, and other hormones (von Horn and Minor, 2019). The construction of an IR cell model is an important way to explore the pathological mechanism and drug development of IR-related diseases.…”

Section: Introductionmentioning

confidence: 99%

Combined Use of Astragalus Polysaccharide and Berberine Attenuates Insulin Resistance in IR-HepG2 Cells via Regulation of the Gluconeogenesis Signaling Pathway

et al. 2019

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Insulin resistance (IR) is likely to induce metabolic syndrome and type 2 diabetes mellitus (T2DM). Gluconeogenesis (GNG) is a complex metabolic process that may result in glucose generation from certain non-carbohydrate substrates. Chinese herbal medicine astragalus polysaccharides and berberine have been documented to ameliorate IR, and combined use of astragalus polysaccharide (AP) and berberine (BBR) are reported to synergistically produce an even better effect. However, what change may occur in the GNG signaling pathway of IR-HepG2 cells in this synergistic effect and whether AP-BBR attenuates IR by regulating the GNG signaling pathway remain unclear. For the first time, we discovered in this study that the optimal time of IR-HepG2 cell model formation was 48 h after insulin intervention. AP-BBR attenuated IR in HepG2 cells and the optimal concentration was 10 mg. AP-BBR reduced the intracellular H 2 O 2 content with no significant effect on apoptosis of IR-HepG2 cells. In addition, a rapid change was observed in intracellular calcium current of the IR-HepG2 cell model, and AP-BBR intervention attenuated this change markedly. The gene sequencing results showed that the GNG signaling pathway was one of the signaling pathways of AP-BBR to attenuate IR in IR-Hepg2 cells. The expression of p-FoxO1 Ser256 and PEPCK protein was increased, and the expression of GLUT2 protein was decreased significantly in the IR-HepG2 cell model, and both of these effects could be reversed by AP-BBR intervention. AP-BBR attenuated IR in IR-HepG2 cells, probably by regulating the GNG signaling Pathway.

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Transcriptome analysis to reveal the mechanism of the effect of Echinops latifolius polysaccharide B on palmitate-induced insulin-resistant

Xia

Gao

et al. 2021

Biomedicine & Pharmacotherapy

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Moderne Konzepte zur dynamischen Konservierung von Leber und Nieren im Rahmen einer Transplantation

Cited by 7 publications

References 59 publications

Astragalus polysaccharide ameliorates insulin resistance in HepG2 cells through activating the STAT5/IGF‐1 pathway

Astragalus polysaccharide ameliorates insulin resistance in HepG2 cells through activating the STAT5/IGF‐1 pathway

Combined Use of Astragalus Polysaccharide and Berberine Attenuates Insulin Resistance in IR-HepG2 Cells via Regulation of the Gluconeogenesis Signaling Pathway

Transcriptome analysis to reveal the mechanism of the effect of Echinops latifolius polysaccharide B on palmitate-induced insulin-resistant

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