Modification of the surface of superparamagnetic iron oxide nanoparticles to enable their safe application in humans

Strehl, Cindy; Maurizi, Lionel; Gaber, Timo; Hoff, Paula; Broschard, Thomas H.; Poole, A. Robin; Hofmann, Heinrich; Buttgereit, Frank

doi:10.2147/ijn.s110579

Cited by 24 publications

(9 citation statements)

References 42 publications

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“…Furthermore, PVA coated SPIONs do not significantly activate or influence human T helper cells and have a negligible influence on T cell apoptosis at a concentration of 100 μgSPIONs/mL after 72 h [19]. Regarding PVA, the covalent binding of this polymer onto the SPION surface significantly decreases some inflammatory effects on same T helper cells [20]. Evaluating cytotoxicity of SPIONs is not a trivial operation.…”

Section: Cytotoxicity Of Spionsmentioning

confidence: 99%

Toxicological Risk Assessment of Emerging Nanomaterials: Cytotoxicity, Cellular Uptake, Effects on Biogenesis and Cell Organelle Activity, Acute Toxicity and Biodistribution of Oxide Nanoparticles

Maurizi¹,

Papa²,

Boudon³

et al. 2018

Unraveling the Safety Profile of Nanoscale Particles and Materials - From Biomedical to Environmental Applications

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The lack of toxicological data on nanomaterials makes it difficult to assess the risk related to their exposure, and as a result further investigation is required. This chapter presents the synthesis of controlled oxide nanoparticles followed by the evaluation of their safety profile or toxicity (iron, titanium and zinc oxides). The controlled surface chemistry, dispersion in several media, morphology and surface charge of these nanoparticles are presented (transmission electron microscopy, dynamic light scattering, zeta potential, X-ray photoelectron spectroscopy). Classical cytotoxic and cellular uptake studies on different cancer cell lines from liver, prostate, heart, brain and spinal cord are discussed. The incidence of nanoparticles on biogenesis and activity of cell organelles is also highlighted, as well as their biodistribution in animal models. The acute toxicity on zebrafish embryo model is also presented. Finally, the stress is put on the influence and the necessity of controlling the protein corona, a layer of plasma proteins physically adsorbed at the surface of such nanoparticles as a result of their presence in the bloodstream (or relevant biological fluids).

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Section: Cytotoxicity Of Spionsmentioning

confidence: 99%

Toxicological Risk Assessment of Emerging Nanomaterials: Cytotoxicity, Cellular Uptake, Effects on Biogenesis and Cell Organelle Activity, Acute Toxicity and Biodistribution of Oxide Nanoparticles

Maurizi¹,

Papa²,

Boudon³

et al. 2018

Unraveling the Safety Profile of Nanoscale Particles and Materials - From Biomedical to Environmental Applications

Self Cite

View full text Add to dashboard Cite

show abstract

“…ION toxicity has been demonstrated to vary considerably and also to depend on cell type and physical-chemical characteristics such as size, shape, presence/type of coating, and stability in biological media (Gupta and Gupta 2005, Mahdavi et al 2013, Strehl et al 2016.…”

Section: A N U S C R I P Tmentioning

confidence: 99%

Toxicological assessment of silica-coated iron oxide nanoparticles in human astrocytes

Fernández-Bertólez

Costa

Brandão

et al. 2018

Food and Chemical Toxicology

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Iron oxide nanoparticles (ION) have great potential for an increasing number of medical and biological applications, particularly those focused on nervous system. Although ION seem to be biocompatible and present low toxicity, it is imperative to unveil the potential risk for the nervous system associated to their exposure, especially because current data on ION effects on human nervous cells are scarce. Thus, in the present study potential toxicity associated with silica-coated ION (S-ION) exposure was evaluated on human A172 glioblastoma cells. To this aim, a complete toxicological screening testing several exposure times (3 and 24 h), nanoparticle concentrations (5-100 μg/ml), and culture media (complete and serum-free) was performed to firstly assess S-ION effects at different levels, including cytotoxicity - lactate dehydrogenase assay, analysis of cell cycle and cell death production - and genotoxicity - H2AX phosphorylation assessment, comet assay, micronucleus test and DNA repair competence assay. Results obtained showed that S-ION exhibit certain cytotoxicity, especially in serum-free medium, related to cell cycle disruption and cell death induction. However, scarce genotoxic effects and no alteration of the DNA repair process were observed. Results obtained in this work contribute to increase the knowledge on the impact of ION on the human nervous system cells.

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“…Unfortunately, the majority of the studies focused on SPIONs neglect that aspect. Surface modifications of SPIONs are mostly aimed at the improvement of their colloidal stability (coating with poly(ethylene glycol)—PEG [ 24 ] or poly(vinyl alcohol)—PVA [ 25 ]), biocompatibility (coating with natural polymers such as alginate [ 26 ] or chitosan [ 18 ]) or the attachment of various biologically active ligands (functionalization with PEG or gold [ 27 ]). However, in terms of the inhibition of iron release, the modification of SPIONs with stable silica coatings seems to be the most appropriate.…”

Section: Introductionmentioning

confidence: 99%

Superparamagnetic Iron Oxide Nanoparticles Modified with Silica Layers as Potential Agents for Lung Cancer Treatment

et al. 2020

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Superparamagnetic iron oxide nanoparticles (SPIONs) are promising drug delivery carriers and hyperthermia agents for the treatment of cancer. However, to ensure their safety in vivo, SPIONs must be modified in order to prevent unwanted iron release. Thus, SPIONs were coated with silica layers of different morphologies: non-porous (@SiO2), mesoporous (@mSiO2) or with a combination of non-porous and mesoporous layers (@SiO2@mSiO2) deposited via a sol–gel method. The presence of SiO2 drastically changed the surface properties of the nanoparticles. The zeta potential changed from 19.6 ± 0.8 mV for SPIONs to −26.1 ± 0.1 mV for SPION@mSiO2. The Brunauer–Emmett–Teller (BET) surface area increased from 7.54 ± 0.02 m2/g for SPIONs to 101.3 ± 2.8 m2/g for SPION@mSiO2. All types of coatings significantly decreased iron release (at least 10 fold as compared to unmodified SPIONs). SPIONs and SPION@mSiO2 were tested in vitro in contact with human lung epithelial cells (A549 and BEAS-2B). Both nanoparticle types were cytocompatible, although some delay in proliferation was observed for BEAS-2B cells as compared to A549 cells, which was correlated with increased cell velocity and nanoparticles uptake.

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Modification of the surface of superparamagnetic iron oxide nanoparticles to enable their safe application in humans

Cited by 24 publications

References 42 publications

Toxicological Risk Assessment of Emerging Nanomaterials: Cytotoxicity, Cellular Uptake, Effects on Biogenesis and Cell Organelle Activity, Acute Toxicity and Biodistribution of Oxide Nanoparticles

Toxicological Risk Assessment of Emerging Nanomaterials: Cytotoxicity, Cellular Uptake, Effects on Biogenesis and Cell Organelle Activity, Acute Toxicity and Biodistribution of Oxide Nanoparticles

Toxicological assessment of silica-coated iron oxide nanoparticles in human astrocytes

Superparamagnetic Iron Oxide Nanoparticles Modified with Silica Layers as Potential Agents for Lung Cancer Treatment

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