Modified E-rosette test for detection of total and active rosette-forming lymphocytes

HLA Antigens and E‐Rosettes in Tetanus Patients

“…The higher values for active E-rosettes as obtained in the present study may be due to the use of red cell free lymphocyte suspension as demonstrated by Bach (1973) and Florey & Peetoom (1976). The values of active and total E-rosettes differed for the related blood donors and laboratory workers, with the former giving lower values.…”

Autologous mixed lymphocyte reaction in lymphoid malignancies. Lack of correlation with disease activity or clinical remission

“…The cells were washed twice with Hank's balanced salt solution (HBSS, GIBCO, Grand Island, NY, USA) and resuspended in the same medium at a concentration of 6 x 10 6 cells/ml. The MNC were mixed vol/vol (1 : l) with a 1% suspension of sheep erythrocytes (SRBC) treated with brome/in (EB) [13]. Briefly, 0.5% bromelin (Calbiochem; La Jolla, CA, USA) was added to a 1% suspension of washed SRBC J. R. Quesada et al: AMLR in Lymphoid Malignancies and immediately centrifuged at 500 g for 15 rain.…”

Subpopulações linfocitarias do liquido cefalorraqueano normal: I. principais registros da literatura

“…É descrito aumento do número de células T ativas, paralelo ao desenvolvimento de reações de hipersensibilidade tardia no teste do PPD 21,22. Os estudos que tentam correlacionar a população T ativa com as outras subclasses de linfócitos são escassos e, muitas vezes, contraditórios 24 . Além disso, em afecções do SNC, o comportamento deste contingente no sangue periférico e no LCR é totalmente diferente quando analisados os dois sistemas em conjunto.5i.…”