Molecular and serological diagnosis of Toxoplasma gondii infection in experimentally infected chickens

Yan, Chao; Chen, Yue; Yuan, Zihao; Lin, Rui-Qing; He, Yong; Yin, Chao; Xu, Min-Jun; Song, Hui-Qun; Zhu, Xing‐Quan

doi:10.1016/j.vetpar.2010.07.011

Cited by 33 publications

(22 citation statements)

References 15 publications

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“…Although it is not possible to confirm the viability of the parasite using PCR testing, the results call into question whether the consumption of this type of meat could be a risk to the consumer, particularly if the meat or viscera are undercooked (MILLAR et al, 2012). Yan et al (2010) studied chickens in China and reported that the organs with the greatest quantity of T. gondii parasites were the heart and lungs, and Aigner et al (2010) demonstrated the amplification of T. gondii DNA in chicken brain and heart samples in Paraná (Brazil). The present study also confirmed positivity in lung and heart samples.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of anti-Toxoplasma gondii antibodies and parasite DNA in backyard chicken breeding in Northeast, Brazil

Fernandes

Cavalcanti

Silva

et al. 2016

Rev. Bras. Parasitol. Vet.

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The aim of the present study was to investigate the occurrence of anti-Toxoplasma gondii antibodies and parasite DNA in backyard chickens bred in the metropolitan area of Recife, Brazil. In total, 212 serum samples were collected from 16 properties, and 12 backyard chickens were collected in the six sanitary districts of Recife. An indirect immunofluorescence assay (IFA) was used to investigate the occurrence of anti-Toxoplasma gondii antibodies. Polymerase chain reaction (PCR) was used to detect T. gondii DNA in brain, heart, liver and lung specimens. Of the samples analyzed by serology, 86/212 (40.56%) were positive; of the samples analyzed by PCR, 2/12 (16.7%) were positive, with both samples positive by both tests (serological and molecular). The presence of antibody anti-T. gondii and parasite DNA in tissues of these animals are worrying aspects for public health because there is a risk of transmission of the parasite to humans through eating undercooked or raw meat. Based on the results, the adoption of preventive measures to prevent the cats access to the chickens creations should be encouraged, since these animals were identified in most of the studied properties.Keywords: Toxoplasmosis, Gallus gallus domesticus, Indirect Immunofluorescence Assay (IFA), Polymerase Chain Reaction (PCR), Brazil. ResumoO objetivo do presente estudo foi investigar a ocorrência de anticorpos anti-Toxoplasma gondii e de DNA do parasito em galinhas de criações domésticas, na região metropolitana de Recife, Brasil. No total, 212 amostras de soro foram coletadas de aves de 16 estabelecimentos e de 12 galinhas de criações domésticas nos seis distritos sanitários de Recife. Para a pesquisa de anticorpos anti-Toxoplasma gondii foi utilizada a Reação de Imunofluorescência Indireta (RIFI). A Reação em Cadeia da Polimerase (PCR) foi utilizada para detectar o DNA de T. gondii em fragmentos de cérebro, coração, fígado e pulmão. Das amostras analisadas por sorologia, 86/212 (40,56%) foram positivas. Das amostras analisadas por PCR, 2/212 (16,7%) foram positivas, em ambos os testes (sorológicos e moleculares). A presença de anticorpos anti-T. gondii e de DNA parasitário nos tecidos desses animais são aspectos preocupantes para saúde pública, porque há o risco de transmissão do parasita para humanos através da ingestão de carne mal cozida ou crua. Com base nos resultados obtidos, a adoção de medidas preventivas que evitem o acesso de gatos às criações de galinhas deve ser incentivada, uma vez que esses animais foram identificados na maioria das propriedades estudadas.

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Section: Discussionmentioning

confidence: 99%

Occurrence of anti-Toxoplasma gondii antibodies and parasite DNA in backyard chicken breeding in Northeast, Brazil

Fernandes

Cavalcanti

Silva

et al. 2016

Rev. Bras. Parasitol. Vet.

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“…MAT is a sensitive and specific technique for measuring T. gondii antibodies, which has been used extensively in experimentally and naturally infected dogs [11,13,28], and other animals [29]. Briefly, MAT was performed with a suspension of Toxoplasma tachyzoites fixed with formalin, serum samples which had been diluted in phosphate buffered saline (PBS, pH 7.2), positive and negative control sera, antigen diluting buffer containing bovine serum albumin (BSA), 2-mercaptoethanol [to deplete the sera of non-specific immunoglobulin (Ig) M antibody], and Evans blue dye solution.…”

Section: Methodsmentioning

confidence: 99%

Seroprevalence of Toxoplasma gondii infection in pet dogs in Lanzhou, Northwest China

Huang

et al. 2011

Parasites Vectors

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BackgroundIn recent years, surveys of Toxoplasma gondii infection in dogs have been reported worldwide, including China. However, little is known about the prevalence of T. gondii in pet dogs in Northwest China. In the present study, the prevalence of T. gondii in pet dogs in Lanzhou, China was investigated using the modified agglutination test (MAT).ResultsIn this survey, antibodies to T. gondii were found in 28 of 259 (10.81%) pet dogs, with MAT titers of 1:20 in 14 dogs, 1:40 in nine, 1:80 in four, and 1:160 or higher in one dog. The prevalence ranged from 6.67% to 16.67% among dogs of different ages, with low rates in young pet dogs, and high rates in older pet dogs. The seroprevalence in dogs >3 years old was higher than that in dogs ≤1 years old, but the difference was not statistically significant (P >0.05). The seroprevalence in male dogs was 12.50% (17 of 136), and in female dogs it was 8.94% (11 of 123), but the difference was not statistically significant (P >0.05).ConclusionsA high prevalence of T. gondii infection was found in pet dogs in Lanzhou, Northwest China, which has implications for public health in this region. In order to reduce the risk of exposure to T. gondii, further measures and essential control strategies should be carried out rationally in this region.

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“…Furthermore, western blotting employing immune mouse sera was exploited to illustrate the virulent (RH, ENT, Martin) and avirulent (RRA, DEG, ME49) antigenic profiles of Toxoplasma strains. We accredit the reader to reviews and articles by (Howe and Sibley, 1995;Appleford and Smith, 2000;D'Souza and Morampudi, 2011;Weilhammer and Rasley, 2011;Dubremetz and Lebrun, 2012 contamination with oocysts from cat feces because of their feeding habits (Ruiz and Frenkel, 1980;Yan et al, 2010). It has been proposed that infected chickens might function as T. gondii reservoirs and an important source of Toxoplasma infection ( (Kaneto et al, 1997;Devada et al, 1998;Yan et al, 2010).…”

Section: Iii) Exploring Early and Late T Gondii Strain Rh Infection mentioning

confidence: 99%

“…We accredit the reader to reviews and articles by (Howe and Sibley, 1995;Appleford and Smith, 2000;D'Souza and Morampudi, 2011;Weilhammer and Rasley, 2011;Dubremetz and Lebrun, 2012 contamination with oocysts from cat feces because of their feeding habits (Ruiz and Frenkel, 1980;Yan et al, 2010). It has been proposed that infected chickens might function as T. gondii reservoirs and an important source of Toxoplasma infection ( (Kaneto et al, 1997;Devada et al, 1998;Yan et al, 2010). Infection of specific pathogen free (SFP) chickens with the T. gondii RH strain induces the production of Toxoplasma -specific antisera which were exploited to find specific molecular markers for more precise serodiagnosis of different stages of infection (i.e.…”

Section: Iii) Exploring Early and Late T Gondii Strain Rh Infection mentioning

confidence: 99%

Immunoproteomic technology offers an extraordinary diagnostic approach for Toxoplasma gondii infection

El‐Ashram

Yin

Barta

et al. 2015

Journal of Microbiological Methods

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Molecular and serological diagnosis of Toxoplasma gondii infection in experimentally infected chickens

Cited by 33 publications

References 15 publications

Occurrence of anti-Toxoplasma gondii antibodies and parasite DNA in backyard chicken breeding in Northeast, Brazil

Occurrence of anti-Toxoplasma gondii antibodies and parasite DNA in backyard chicken breeding in Northeast, Brazil

Seroprevalence of Toxoplasma gondii infection in pet dogs in Lanzhou, Northwest China

Immunoproteomic technology offers an extraordinary diagnostic approach for Toxoplasma gondii infection

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