Molecular characterisation of hepatitis B virus in the resident Chinese population in Panama City

Martínez, Alexander A.; Zaldívar, Yamitzel; Hong, Chen Ch; Alvarado-Mora, Mónica Viviana; Smith, R. W.; Ortiz, Alma; Pinho, João Renato Rebello; Cristina, Juan; Pascale, Juan Miguel

doi:10.1590/s0074-02762013000500002

Cited by 3 publications

(3 citation statements)

References 48 publications

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“…This study showed that only genotypes from Asia (Genotypes B and C) are circulating in this group [13]. Still, there is a lack in the knowledge regarding the diversity and molecular characteristics of this virus in the country.…”

Section: Introductionmentioning

confidence: 84%

“…For the preliminary genotyping, samples with detectable viremia were subjected to a nested PCR using a high proof reading enzyme mixture (Platinum PCR Super Mix High Fidelity, LifeTech, USA), that yielded a 879 bp fragment. Sequencing was performed in an automated DNA sequencer (ABI 3130xl, LifeTech, USA) using primers previously described [13].…”

Section: Methodsmentioning

confidence: 99%

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High Diversity of Hepatitis B Virus Genotypes in Panamanian Blood Donors: A Molecular Analysis of New Variants

et al. 2014

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Hepatitis B Virus (HBV) is an infectious agent that causes more than half of the cases of liver disease and cancer in the world. Globally there are around 250 million people chronically infected with this virus. Despite 16% of the cases of liver disease in Central America are caused by HBV, the information regarding its genetic diversity, genotypes and circulation is scarce. The purpose of this study was to evaluate the genetic variability of the HBV genotypes from HBV-DNA positive samples obtained from screening blood donors at the Social Security System of Panama and to estimate its possible origin. From 59,696 blood donors tested for HBV infection during 2010–2012, there were 74 HBV-DNA positive subjects. Analysis of the partial PreS2-S region of 27 sequences shows that 21% of the infections were caused by genotype A, 3% by genotype D and 76% by genotype F. In addition, we were able to confirm circulation of six sub-genotypes A1, A2, A3, D4, F3, F1 and a proposed new sub-genotype denominated F5pan. We found a confinement of sub-genotypes F1 and F5pan to the western area of Panama. The tMRCA analysis suggests a simultaneous circulation of previously described sub-genotypes rather than recent introductions of the Panamanian sub-genotypes in the country. Moreover, these results highlight the need of more intensive research of the HBV strains circulating in the region at the molecular level. In conclusion, Panama has a high HBV genotype diversity that includes a new proposed sub-genotype, an elevated number of PreCore-Core mutations, and confinement of these variants in a specific geographical location.

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Section: Introductionmentioning

confidence: 84%

Section: Methodsmentioning

confidence: 99%

High Diversity of Hepatitis B Virus Genotypes in Panamanian Blood Donors: A Molecular Analysis of New Variants

et al. 2014

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“…There is a high prevalence of genotypes A and D as a reflection of the migratory movements that occurred during the colonial period, especially subtype A1, related to the slave trade [31,32], and A2 and several subtypes of genotype D, associated with European settlers. Strains of genotypes B and C have been isolated in Peru and Panama, as well as in Argentina and Brazil [33][34][35]. Genotypes A, H and G have been detected in Mexico, while genotypes A and H have been found in Nicaragua.…”

Section: Introductionmentioning

confidence: 99%

Genetic variability of hepatitis B virus in Uruguay: D/F, A/F genotype recombinants

López¹,

Flichman

Mojsiejczuk

et al. 2015

Arch Virol

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Hepatitis B virus (HBV) infection is a serious global health problem. Approximately 2 billion people worldwide have been infected, and approximately 350 million individuals currently suffer from HBV-induced chronic liver infection, which causes 600,000 deaths annually from chronic hepatitis, cirrhosis and hepatocellular carcinoma. HBV is classified in eight genotypes (A-H), and two more have been proposed (I-J). In this paper, complete genome sequences of nine Uruguayan HBV are reported. Five samples belong to genotype F1b and one to genotype A2. Three HBV recombinants were detected: A1/F1b, A2/F1b and D3/F1b. The following mutations were detected: a G1896A substitution, a 33-nucleotide deletion from position 2896 to 2928 in the Pre-S1 region involving Pre-S1 residues 3-13, a 33-nt deletion in the Pre-S1 region involving nt 2913-2945 and Pre-S1 residues 9-19. More F genotypes strains than expected were detected in this study, supporting the hypothesis that there are more people of indigenous origin than declared in our population. Also, one third of the samples analyzed were recombinants. This cannot be explained by the low HBV prevalence in Uruguay, but a high HBV infection rate in drug addicts and dialysis patients could act in favor of multiple-genotype HBV infections that could lead to recombination.

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Hepatitis B virus burden in developing countries

Zampino¹

2015

WJG

267

192

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Hepatitis B virus (HBV) infection has shown an intermediate or high endemicity level in low-income countries over the last five decades. In recent years, however, the incidence of acute hepatitis B and the prevalence of hepatitis B surface antigen chronic carriers have decreased in several countries because of the HBV universal vaccination programs started in the nineties. Some countries, however, are still unable to implement these programs, particularly in their hyperendemic rural areas. The diffusion of HBV infection is still wide in several low-income countries where the prevention, management and treatment of HBV infection are a heavy burden for the governments and healthcare authorities. Of note, the information on the HBV epidemiology is scanty in numerous eastern European and Latin-American countries. The studies on molecular epidemiology performed in some countries provide an important contribution for a more comprehensive knowledge of HBV epidemiology, and phylogenetic studies provide information on the impact of recent and older migratory flows.

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Molecular characterisation of hepatitis B virus in the resident Chinese population in Panama City

Cited by 3 publications

References 48 publications

High Diversity of Hepatitis B Virus Genotypes in Panamanian Blood Donors: A Molecular Analysis of New Variants

High Diversity of Hepatitis B Virus Genotypes in Panamanian Blood Donors: A Molecular Analysis of New Variants

Genetic variability of hepatitis B virus in Uruguay: D/F, A/F genotype recombinants

Hepatitis B virus burden in developing countries

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