Molecular characterization of Kenkatha and Gaolao (Bos indicus) cattle breeds using microsatellite markers

Abstract

One hundred forty–five individuals from two cattle breeds, Kenkatha and Gaolao, in India were studied using 25 fluorescently–labelled microsatellite markers. Genetic diversities within and between populations were studied. A total of 197 and 239 distinct alleles were identified across 25 microsatellite loci in Kenkatha and Gaolao cattle, respectively. Means of observed and expected heterozygosity were found to be 0.47 ± 0.24 and 0.62 ± 0.21 in Kenkatha, and 0.53 ± 0.17 and 0.68 ± 0.14 in Gaolao cattle, respectively. The average PIC (Polymorphic Information Content) value was found to be 0.59 ± 0.21 for Kenkatha and 0.65 ± 0.15 for Gaolao cattle. The mean fixation index (FIS) was 0.2121 for Gaolao and 0.2248 for Kenkatha cattle. Mean FIS, mean FIT and mean FST (F–statistics) values were found to be 0.2318, 0.2487 and 0.0219, respectively. Nei’s standard genetic distance value between Kenkatha and Gaolao breeds was 0.0852. The present study indicates that there is a substantial shortfall, 21.21 % and 22.48 %, of heterozygotes in Gaolao and Kenkatha cattle populations, respectively; and little genetic differentiation (2.19%) between the two breeds.

Genetic Diversity of Gorontalo Local Cattle Based on Microsatellite DNA

“…Allele size of the eight markers (MM8, INRA063, BM1818, ILSTS054, ILSTS005, HEL5, ILSTS006 and ILSTS011) ranged from 125-169bp, 163-215bp, 213-285bp, 110-167bp, 160-192bp, 127-193bp, 260-329bp and 240-270bp, respectively in Siwa and Farafra cattle populations (Table 2). Results agree with the selective standard of the microsatellite loci (the Secondary Guidelines for Development of National Farm Animal Genetic Resources using reference Microsatellite given by FAO, 2004).In Gaolao and Kenkatha cattle, Chaudhari et al (2009) found that allele size ranged from 118-150bp, 170-188bp, 258-280bp, 176-190bp, 279-301bp and260-272bp with MM8, INRA063, BM1818, ILSTS005, ILSTS006 andILSTS011, respectively. Gralak et al (2004) reported that the size ranged from 262-264bp, 282bp with BM1818 and ILSTS006, respectively in European bison bovine.…”

“…recorded that the number of observed alleles varied between 3 (ILSTS011) to 10 (ILSTS034) with an overall mean number of 5.95±1.9 alleles per locus. Chaudhari et al (2009) observed a total of 239 and 197 distinct alleles with mean values of 9.52 and 7.92 in Gaolao and Kenkatha cattle, respectively. They also, reported that alleles observed per locus ranged between 5 (loci ILSTS006 and ILSTS030) and 15 (locus ILSTS034) and between 4 (locus ILSTS006) and 14 (loci BM1824 and ILSTS006) in Gaolao and Kenkatha cattle, respectively.…”

“…reported that the averaged of observed heterozygosity was 0.540±0.171 over the 21 loci, while the average of expected heterozygosity ranged from 0.530 (ETH225) to 0.825 (INRA035) with an overall mean of 0.685±0.100 in Kenkatha population. Moreover, Chaudhari et al (2009) recorded that the observed heterozygosity (Ho) ranged between 0.014 (ILSTS006) to 0.7800 (ILSTS034) in Kenkatha and between 0.1000 (ILSTS006) to 0.7500 (ILSTS030) in Gaolao cattle, while the expected heterozygosity (He) ranged between 0.1523 (ETH3) to 0.8881 (ILSTS034) in Kenkatha and between 0.3330 (ETH152) to 0.9029 (ILSIS034) in Gaolao cattle. They also reported that the means values of observed and expected heterozygosities were 0.47 ± 0.24 and 0.62 ± 0.21, respectively in Kenkatha and 0.53 ± 0.17 and 0.68 ± 0.14, respectively in Gaolao cattle.…”

Genetic diversity of bovine populations raised in Senegal

“…The other populations had average heterozygosities between 0.650 and 0.737. These results were very similar to those observed in Gobra populations from the groundnut basin, Moorish from the river valley and N'dama from the CRZ of Kolda in Senegal (Ndiaye et al., 2015); taurine breeds or zebu breeds found in Africa (Dayo et al., 2009; Freeman et al., 2004; Foulley & Ollivier, 2006; MacHugh et al., 1997; Ngono Ema et al., 2015); Asian zebu (Chaudhari et al., 2009; Sharma et al., 2009; Shi et al., 2010; Suh et al., 2014; Singh et al., 2015); European cattle breeds (Dayo et al., 2009; Freeman et al., 2004; Foulley & Ollivier, 2006; MacHugh et al., 1997) and Brazilian breeds (da Silva Filho et al., 2014). This indicates a high genetic diversity observed across the loci studied.…”

“…Asian zebu (Chaudhari et al, 2009;Sharma et al, 2009;Shi et al, 2010;Suh et al, 2014;Singh et al, 2015); European cattle breeds (Dayo et al, 2009;Freeman et al, 2004;Foulley & Ollivier, 2006;MacHugh et al, 1997) and Brazilian breeds (da Silva Filho et al, 2014). This indicates a high genetic diversity observed across the loci studied.…”