Molecular Cloning of a<i>Poria cocos</i>Protein That Activates Th1 Immune Response and Allays Th2 Cytokine and IgE Production in a Murine Atopic Dermatitis Model

Lu, Yating; Kuan, Yen-Chou; Chang, Hui-Hsin; Sheu, Fuu

doi:10.1021/jf405507e

Cited by 26 publications

(16 citation statements)

References 32 publications

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“…The P.cocos is widely used as a traditional medicine in treatment of Jaundice. It extract has beneficial effects on inflammation, cancer and immune function [8][9][10][11]. The combination of P.cocos and western drug for patients with Jaundice also receive a good therapeutic effect.…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics Analysis on Molecular Mechanism of Poria Cocos in Treatment of Jaundice

Si¹,

Nie²,

Jiang³

et al. 2016

Proceedings of the 2016 International Conference on Computer Engineering, Information Science &Amp; Application Technology (ICC

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Abstract. Objective: To study the molecular mechanism of P.cocos in the treatment of Jaundice by building molecular networks and comparing canonical pathways. Methods: Target proteins of P.cocos related genes of Jaundice were searched from Pubchem and Gene databases online respectively. Molecular networks and canonical pathways comparison analyses were performed by IPA. Results: The molecular networks of P.cocos and Jaundice were complex and multifunctional. The 14 target proteins of P.cocos and 33 Homo sapiens genes of Jaundice were found in databases. There were 56 common pathways both related networks. P.cocos could regulate endothelial differentiation Tumor Necrosis Factor (TNF), Interleukin-1β (IL-1β) and Interleukin-6 (IL-6) in these pathways. Conclusions: P.cocos treat Jaundice by regulatingmany effective nodes of Metabolism pathway and Immunity related pathways.

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Section: Discussionmentioning

confidence: 99%

Bioinformatics Analysis on Molecular Mechanism of Poria Cocos in Treatment of Jaundice

Si¹,

Nie²,

Jiang³

et al. 2016

Proceedings of the 2016 International Conference on Computer Engineering, Information Science &Amp; Application Technology (ICC

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“…PCP stimulated mouse peritoneal macrophages (RAW264.7) by interacting with toll-like receptor 4 and subsequently activating the NFκB signaling pathway. Oral administration of PCP reduced the production of serum total IgG1 and OVA-specific IgG1 and upregulated the serum OVA-specific IgG2a and splenic Th1-related cytokine and downregulated IL-4 and IgE levels in atopic dermatitis mice [74]. P. antler tray proteins (PSAB) is a mixture of 5 proteins with a molecular mass of 10 to 70 kDa and includes 17 amino acids.…”

Section: Biological Activities Of Biomacromolecules Based On Its Effimentioning

confidence: 99%

Pharmacodynamic material basis of traditional Chinese medicine based on biomacromolecules: a review

et al. 2020

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Biomacromolecules, the first components of bioactive substances in traditional Chinese medicines (TCM) have wide bioactivity-related efficacy but have not yet been fully appreciated compared to small molecule components. The present review brings a novel and systemic point of view to deepen the understanding of the pharmacodynamic material basis of TCM based on biomacromolecules (polysaccharides, proteins and nucleic acids). Biomacromolecules have been, are and will have considerable roles in the efficacy of Chinese medicine, as evidenced by the number of biological activities related to traditional clinical efficacy. The direct and indirect mechanisms of biomacromolecules are further accounted for in a variety of neurotransmitters, hormones, and immune substances to maintain immune function in both sensitive and stable equilibrium. The biological functions of biomacromolecules have been elaborated on in regard to their roles in the process of plant growth and development to the relationship between primary metabolism and secondary metabolism and to the indispensable role of polysaccharides, proteins, and nucleic acids in the quality formation of TCM. Understanding the functional properties and mechanisms of biological macromolecules will help to demystify the drug properties and health benefits of TCM.

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“…Originally, its sclerotium has been used as an important medicinal herb in several Asian countries, particularly China, Japan and Korea, for its sedative, diuretic, anti-depressant and tonic activities (9,10). Recent studies have indicated that the extracts and components of this fungus display a variety of biological activities, such as anti-fungal and anti-bacterial (11), anti-oxidant (12,13), neuroprotective (14), anti-hypertonic (15), anti-inflammatory (16)(17)(18)(19), anti-angiogenic (20,21), immunomodulatory (22,23) and anticancer effects (24,25). Although several triterpenoids from P. cocos have been isolated and were demonstrated to have a cytotoxic effect against a number of human cancer cell types (26)(27)(28)(29), the efficacy and mechanism of the whole extract in cancer treatment have not been systemically evaluated.…”

Section: Introductionmentioning

confidence: 99%

Induction of apoptosis by an ethanol extract of Poria cocos Wolf. in human leukemia U937 cells

Choi

2015

Oncology Reports

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Poria cocos Wolf., which belongs to the Polyporaceae family, has been widely used as an Oriental traditional herbal medicine for centuries. Its sclerotium has been reported to possess a wide spectrum of pharmacological activities, including free-radical scavenging, anti-viral, anti-microbial, anti-inflammatory and anticancer activities. However, the cellular and molecular mechanisms of apoptosis induction by P. cocos in human cancer cells are poorly understood. In the present study, we investigated the pro-apoptotic potential of an ethanol extract of P. cocos sclerotium (EEPC) in human leukemia U937 cells in vitro. We found that EEPC induced anti-proliferative effects in U937 cells in a concentration- and time-dependent manner, which was due to apoptotic induction, as evident from morphological changes and flow cytometric assays. EEPC-induced apoptosis of U937 cells was associated with an increase in the Bax:Bcl-2 ratio, the release of cytochrome c to the cytosol, and a decrease in the expression of an inhibitor of the apoptosis family of proteins. The events were accompanied by activation of caspase-8, -9 and -3, and cleaved poly(ADP-ribose) polymerase, suggesting the involvement of both the intrinsic and extrinsic apoptotic cascades. In addition, the overexpression of Bcl-2 caused a significant attenuation of EEPC-induced caspase activation, degradation of PARP, and the collapse of mitochondrial membrane potential, and thereby reversed EEPC-induced cell apoptosis and growth inhibition. Collectively, these data provide insights into the molecular mechanisms underlying EEPC-induced apoptosis in U937 cells, suggesting that EEPC may be a new therapeutic option for the treatment of leukemia.

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Molecular Cloning of aPoria cocosProtein That Activates Th1 Immune Response and Allays Th2 Cytokine and IgE Production in a Murine Atopic Dermatitis Model

Cited by 26 publications

References 32 publications

Bioinformatics Analysis on Molecular Mechanism of Poria Cocos in Treatment of Jaundice

Bioinformatics Analysis on Molecular Mechanism of Poria Cocos in Treatment of Jaundice

Pharmacodynamic material basis of traditional Chinese medicine based on biomacromolecules: a review

Induction of apoptosis by an ethanol extract of Poria cocos Wolf. in human leukemia U937 cells

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