Molecular diversity of 1-aminocyclopropane-1-carboxylate (ACC) deaminase producing PGPR from wheat (Triticum aestivum L.) rhizosphere

Gontia‐Mishra, Iti; Sapre, Swapnil; Kachare, Satish; Tiwari, Sharad

doi:10.1007/s11104-016-3119-3

Cited by 103 publications

(33 citation statements)

References 49 publications

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“…Myxobacteria include Gram-negative gliding bacteria with predatory features, mostly due to the direct cell to cell contact, production of hydrolytic enzymes and secondary metabolites with antibiotic activity 81 . The third module in tall cultivars show one module hub (OTU 575 -classified as belonging to the genus Chryseobacterium) which has previously been described in the rhizosphere of wheat [14, 82;], and these bacteria have been shown to display plant growth-promotion 82 and are enriched in the presence of 2,4-DAPG-producing species, such as Pseudomonas fluorescens 83 . Interestingly, the other module hub identified in the same module as Chryseobacterium was OTU 171 (assigned to the genus Pseudomonas) and they positively co-occur, meaning that their abundance changed along the same trend but not necessarily meaning they directly interact with each other 74 .…”

Section: Resultsmentioning

confidence: 96%

Wheat dwarfing influences selection of the rhizosphere microbiome

Kavamura

Robinson

Hughes

et al. 2020

Sci Rep

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The development of dwarf wheat cultivars combined with high levels of agrochemical inputs during the green revolution resulted in high yielding cropping systems. However, changes in wheat cultivars were made without considering impacts on plant and soil microbe interactions. We studied the effect of these changes on root traits and on the assembly of rhizosphere bacterial communities by comparing eight wheat cultivars ranging from tall to semi-dwarf plants grown under field conditions. Wheat breeding influenced root diameter and specific root length (SRL). Rhizosphere bacterial communities from tall cultivars were distinct from those associated with semi-dwarf cultivars, with higher differential abundance of Actinobacteria, Bacteroidetes and Proteobacteria in tall cultivars, compared with a higher differential abundance of Verrucomicrobia, Planctomycetes and Acidobacteria in semi-dwarf cultivars. Predicted microbial functions were also impacted and network analysis revealed a greater level of connectedness between microbial communities in the tall cultivars relative to semi-dwarf cultivars. Taken together, results suggest that the development of semi-dwarf plants might have affected the ability of plants to recruit and sustain a complex bacterial community network in the rhizosphere.

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Section: Resultsmentioning

confidence: 96%

Wheat dwarfing influences selection of the rhizosphere microbiome

Kavamura

Robinson

Hughes

et al. 2020

Sci Rep

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“…In the study of Okunishi et al 22 the genera Burkholderia, Enterobacter, and Pantoea were reported to be abundant in mature rice, which was different from our observations with the scented black rice plant. Some of the genera which were detected in the core microbiome of the scented black rice plant are well-established plant growth-promoting rhizobacteria (PGPR), that are known to promote plant secondary metabolites accumulation and consequently, antioxidant capacity 23 , 24 such as Bradyrhizobium, Streptomyces, Rhizobium. These genera had higher abundance in the root of the mature stage in all the three varieties- Amubi, Poreiton, and Sempak .…”

Section: Discussionmentioning

confidence: 99%

Host specific endophytic microbiome diversity and associated functions in three varieties of scented black rice are dependent on growth stage

Singha

Singh

Pandey

2021

Sci Rep

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The compositional and functional role of the endophytic bacterial community, associated with black scented rice, in correlation with its antioxidant property has been elucidated. Community dissimilarity analysis confirmed the overlapping of community in shoot and root tissues at the young stage, but not in mature plants. Proteobacteria was the most abundant phylum, in which Agrobacterium, Pleomorphomonas, Bradyrhizobium, Novasphingobium, Caulobacter were the most abundant genera, followed by Cyanobacteria and Planctomycetes in all three different varieties of the black rice. The antioxidant activity of mature plants was found to be higher in comparison to young plants. Intrinsically, the relative abundance of Pleomorphomonas and Streptomyces was positively correlated with total phenol content, while Gemmata, unclassified Pirellulaceae, unclassified Stramenopiles positively correlated with total flavonoid content and negatively correlated with Free radical scavenging activity. Accordingly, functional metagenome analysis of the endophytic microbiome revealed that naringenin -3-dioxygenase and anthocyanidin 3-O-glucosyltransferase for phenylpropanoid (flavonoid and anthocyanin) synthesis were abundant in the endophytic microbiome of mature plants. Specific enrichment of the antioxidant producing genes in the mature plant endophytic microbiome was assigned to some bacteria such as Streptomyces, Pantoea which might have contributed to the common pathway of flavonoid synthesis. The genomes of endophytic isolates Kluyvera sp.PO2S7, Bacillus subtilis AMR1 and Enterobacter sp. SES19 were sequenced and annotated, and were found to have genes for phenylpropanoid synthesis in their genomes.

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“…and Enterobacter sp., respectively. The identified bacterial genera have been reported as plant growth promoting bacteria [ 8 , 73 – 76 ].…”

Section: Discussionmentioning

confidence: 99%

Phosphate solubilizing bacteria with glucose dehydrogenase gene for phosphorus uptake and beneficial effects on wheat

et al. 2018

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The aim of this study was to isolate, characterize and use phosphate solubilizing bacteria to enhance the bioavailability of insoluble Ca-phosphate for wheat plants. For this purpose, 15 phosphorus solubilizing bacteria (PSB) were isolated from wheat rhizospheric soils of Peshawar and southern Punjab region, Pakistan. These isolates were identified using light microscopy and 16S rRNA gene. Among the isolated bacteria, two strains (Pseudomonas sp. MS16 and Enterobacter sp. MS32) were the efficient P solubilizers based on their P solubilization activity determined qualitatively (solubilization index 3.2–5.8) as well as quantitatively (136–280 μg mL-1). These two strains produced indole-3-acetic acid (25.6–28.1 μg mL-1), gibberellic acid (2.5–11.8), solubilized zinc compounds (SI 2.8–3.3) and showed nitrogenase and 1-Aminocyclopropane-1-carboxylic acid deaminase activity in vitro. Phosphate solubilization activity of Pseudomonas sp. MS16 was further validated by amplification, sequencing and phylogenetic analysis of glucose dehydrogenase (gcd) gene (LT908484) responsible for P solubilization. Response Surface Methodology for large-scale production was used to find optimal conditions (Temperature 22.5°C, pH 7) for P solubilization. Glucose was found to support higher P solubilization in vitro. In an in vitro experiment, PSB treated wheat seedlings improved germination and seedling vigor (11% increases) as compared to un-inoculated control. Rhizoscanning of these seedlings showed an increase in various root growth parameters. Wheat inoculation with selected strain MS16 showed pronounced effect on grain yield in pot (38.5% increase) and field (17–18% increase) experiments compared to non-inoculated control. Root colonization by PSB through Florescent in situ Hybridization and Confocal Laser Scanning Microscopy confirmed their rhizosphere competence in soil. BOX-PCR confirmed the re-isolated colonies of Pseudomonas sp. MS16. The results indicated that gluconic acid producing Pseudomonas sp. MS16 from un-explored soils may be cost effective and environment friendly candidate to improve plant growth and phosphorous uptake by wheat plants.

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Molecular diversity of 1-aminocyclopropane-1-carboxylate (ACC) deaminase producing PGPR from wheat (Triticum aestivum L.) rhizosphere

Cited by 103 publications

References 49 publications

Wheat dwarfing influences selection of the rhizosphere microbiome

Wheat dwarfing influences selection of the rhizosphere microbiome

Host specific endophytic microbiome diversity and associated functions in three varieties of scented black rice are dependent on growth stage

Phosphate solubilizing bacteria with glucose dehydrogenase gene for phosphorus uptake and beneficial effects on wheat

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