Molecular Evaluation of <i>Garcinia kola</i> Heckel Accessions Using RAPD Markers

Abstract

The genetic relationships among twenty-five accessions of Garcinia kola using six Random Amplified Polymorphic (RAPD) primers were evaluated in this study. The highest volume of total genomic DNA (2218/µl) was recorded in ON4 from Ikare, while the highest DNA concentration of 1.93 gl was found in OS3 from Ilesa. The highest Polymorphic Information Content (PIC) and gene diversity of 93.77% and 0.94 respectively were revealed by primer OPO2 compared to other primers. The dendogram generated from Unweighted Pair Group with Mean Average (UPGMA) clustering delineated two groups, A and B, consisting of 21 and 4 accessions respectively. This study clearly showed the level of molecular diversity in the accessions and the information provided could be utilized for genetic improvement and conservation of Garcinia kola.

Genome-wide genetic diversity and population structure of Garcinia kola (Heckel) in Benin using DArT-Seq technology

“…Discriminant analysis of principal components (DAPC) and the UPGMA analyses partitioned the 100 G. kola individuals into two principal genetic clusters. Hierarchical clustering analysis performed on G. kola accessions in Nigeria also revealed two clusters [31]. However, in the present study, an admixture of almost all the populations was noted within the two clusters.…”

“…Low to moderate levels of observed heterozygosity (0.223-0.248) in the analyzed populations in this study point to high heterozygote deficiency. In contrast, very high heterozygosity was observed in G. kola accessions from Nigeria [31]. The low heterozygosity may be due to a severe bottleneck event that occurred during domestication and selection [33].…”

“…The average PIC is also close to the value obtained for SNP markers identified using GBS in the study of rice [29] and wheat [30] diversity. A previous study on G. kola in Nigeria using RAPD markers reported a PIC value of 0.93 [31]. The bi-allelic nature of DArT-SNP markers for…”

“…A previous study on G . kola in Nigeria using RAPD markers reported a PIC value of 0.93 [ 31 ]. The bi-allelic nature of DArT-SNP markers for which the maximum value for PIC is 0.5, compared to multi-allelic RAPD markers with the maximum PIC value of 1 [ 32 ] can explain the difference in PIC values observed in the two marker systems.…”

“…In contrast, very high heterozygosity was observed in G . kola accessions from Nigeria [ 31 ]. The low heterozygosity may be due to a severe bottleneck event that occurred during domestication and selection [ 33 ].…”