Molecular identification of Diphyllobothrium latum and a brief review of diphyllobothriosis in China

Guo, Aijiang; Liu, Kan; Gong, Wei; Luo, Xuenong; Yan, Hong‐Bin; Zhao, Song-Buo; Hu, Songnian; Jia, Wan‐Zhong

doi:10.2478/s11686-012-0036-3

Cited by 12 publications

(8 citation statements)

References 11 publications

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“…PCR followed by sequencing using universal primers to amplify the ribosomal ITS1, ITS2, 5.8S, and SSU rDNA sequences or mitochondrial genes is used to identify and confirm the species identity of tapeworms found in individual cases as well as interspecies variation to construct phylogenetic relationships (408)(409)(410)(411). Sequence analysis of the ITS1, cox1, and paramyosin gene sequences from H. nana isolates from northwest Australia provided genetic support that the life cycle involves mainly humanto-human transmission (412).…”

Section: Cestodesmentioning

confidence: 99%

Molecular Testing for Clinical Diagnosis and Epidemiological Investigations of Intestinal Parasitic Infections

2014

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SUMMARY Over the past few decades, nucleic acid-based methods have been developed for the diagnosis of intestinal parasitic infections. Advantages of nucleic acid-based methods are numerous; typically, these include increased sensitivity and specificity and simpler standardization of diagnostic procedures. DNA samples can also be stored and used for genetic characterization and molecular typing, providing a valuable tool for surveys and surveillance studies. A variety of technologies have been applied, and some specific and general pitfalls and limitations have been identified. This review provides an overview of the multitude of methods that have been reported for the detection of intestinal parasites and offers some guidance in applying these methods in the clinical laboratory and in epidemiological studies.

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Section: Cestodesmentioning

confidence: 99%

Molecular Testing for Clinical Diagnosis and Epidemiological Investigations of Intestinal Parasitic Infections

2014

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“…Because of the morphologic similarities between different Diphyllobothrium tapeworms, species‐specific diagnosis is challenging (Chen et al., ). More recent studies have preliminarily identified two Diphyllobothrium specimens by conventional morphological methods, and the identity of species was then confirmed by a molecular approach involving the 18S rDNA partial sequence, complete sequences of ITSs and 5.8S rDNA, partial sequences of mitochondrial cox1, and the mitochondrial NADH dehydrogenase subunit 5 (nad5) (Guo et al., ). To date, no reports of serologic or coproantigenic diagnostics of diphyllobothriosis exist.…”

Section: Helminthsmentioning

confidence: 99%

Surveillance and diagnosis of zoonotic foodborne parasites

Emameh

Purmonen

Sukura

et al. 2017

Food Science & Nutrition

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Foodborne parasites are a source of human parasitic infection. Zoonotic infections of humans arise from a variety of domestic and wild animals, including sheep, goats, cattle, camels, horses, pigs, boars, bears, felines, canids, amphibians, reptiles, poultry, and aquatic animals such as fishes and shrimp. Therefore, the implementation of efficient, accessible, and controllable inspection policies for livestock, fisheries, slaughterhouses, and meat processing and packaging companies is highly recommended. In addition, more attention should be paid to the education of auditors from the quality control (QC) and assurance sectors, livestock breeders, the fishery sector, and meat inspection veterinarians in developing countries with high incidence of zoonotic parasitic infections. Furthermore, both the diagnosis of zoonotic parasitic infections by inexpensive, accessible, and reliable identification methods and the organization of effective control systems with sufficient supervision of product quality are other areas to which more attention should be paid. In this review, we present some examples of successful inspection policies and recent updates on present conventional, serologic, and molecular diagnostic methods for zoonotic foodborne parasites from both human infection and animal‐derived foods.

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“…Therefore, some cases of diphyllobothriosis reported in mainland China were probably caused by infections with D. nihonkaiense ; case-patient 12 ( 13 ) in this study is considered to have had such a case. However, a recent report stating that the causative species of 2 diphyllobothriosis cases in northeastern China was D. latum which suggests that D. latum is also indigenous to mainland China ( 14 ). …”

Section: The Studymentioning

confidence: 99%

“…In mainland China, 15 cases of diphyllobothriosis among humans have been reported since the first report in 1927 through 2012; the etiologic species was identified as D. latum by morphologic characteristics ( 11 – 13 ; Table) and molecular markers ( 14 , 15 ). No cases of diphyllobothriosis had been reported in large cities such as Beijing and Shanghai during 1954–2007 ( 11 ).…”

mentioning

confidence: 99%