Molecular tools for identification of Penicillium starter cultures used in the food industry

Dupont, Joëlle; Magnin, Sandrine; Marti, Alessandra; Brousse, María M.

doi:10.1016/s0168-1605(99)00055-0

Cited by 46 publications

(20 citation statements)

References 25 publications

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“…For P. griseoroseum, the size of the amplified ITS region was of 594 bp and nucleotide sequence deposited into GenBank with accession number AY425983. The size of the amplified ITS region is similar to that reported for other Penicillium species (8,14,39). ITS sequence of P. expansum and P. griseoroseum were aligned with ITS sequences of others Penicillium species (Fig.…”

Section: Resultssupporting

confidence: 83%

“…It was therefore expected that very distinct patterns would be observed when used to study different species, but identical patterns from RAPD analysis of different Penicillium spp. with 21 primers was reported by Dupond et al (14). Molecular characterization based on the RAPD markers among 10 Penicillium species was reported by Pereira et al (25).…”

Section: Resultsmentioning

confidence: 91%

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Morphological and molecular differentiation of the pectinase producing fungi Penicillium expansum and Penicillium griseoroseum

Cardoso¹,

Queiroz²,

Pereira³

et al. 2007

Braz. J. Microbiol.

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Two species from the genus Penicillium, Penicillium expansum and P. griseoroseum (Brasilian isolates) were characterized morphologic and molecularlly. Morphological variability was detected among isolates in regard to colony morphology and to conidia coloration. The molecular characterization was based on the RAPD markers, telomeric fingerprinting and ITS sequencing. A total of 78 RAPD primers were used and 8 presented differences in band patterns with 54% of the amplified polymorphic fragments. The monomorphic fragments of 600 bp (P. expansum) and 594 bp (P. griseoroseum) were amplified. The only internal transcribed spacer region variation detected between the two species was the additional six initial nucleotides. The analysis by telomeric fingerprinting showed polymorphism between both species and the chromosome minimal numbers estimated were three. The polymorphism observed in the organization of the subtelomeric region in the genome of two Penicillium species within the high homogeneous Penicillium subgenus is for the first time reported and perhaps can be employed in future phylogenetic studies.

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Section: Resultssupporting

confidence: 83%

Section: Resultsmentioning

confidence: 91%

Morphological and molecular differentiation of the pectinase producing fungi Penicillium expansum and Penicillium griseoroseum

Cardoso¹,

Queiroz²,

Pereira³

et al. 2007

Braz. J. Microbiol.

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“…with acute deficiencies of vitamins B 6 , B 12 and folic acid as co-enzymes for L-methionine cycle enzymes [16]. However, the main cause of cystathioninuria is the lack in genetical expression of CGL in human tissues [26,38].…”

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confidence: 99%

Screening, morphological and molecular characterization of fungi producing cystathionine γ-lyase

El-Sayed¹,

Khalaf²,

abdelhamid³

et al. 2015

Acta Biologica Hungarica

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The potency for production of cystathionine γ-lyase (CGL) by the fungal isolates was screened. Among the tested twenty-two isolates, Aspergillus carneus was the potent CGL producer (6.29 U/mg), followed by A. ochraceous (6.03 U/mg), A. versicolor (2.51 U/mg), A. candidus (2.12 U/mg), A. niveus and Penicillium notatum (2.0 U/mg). The potent six isolates producing CGL was characterized morphologically, A. carneus KF723837 was further molecularly characterized based on the sequence of 18S-28S rDNA. Upon sulfur starvation, the yield of A. carneus extracellular CGL was increased by about 1.7-and 4.1-fold comparing to non-sulfur starved and L-methionine free medium, respectively. Also, the uptake of L-methionine was duplicated upon sulfur starvation, assuming the activation of specific transporters for L-methionine and efflux of CGL. Also, the intracellular thiols and GDH activity of A. carneus was strongly increased by S starvation, revealing the activation of in vivo metabolic antioxidant systems. upon irradiation of A. carneus by 2.0 kGy of γ-rays, the activity of CGL was increased by two-fold, regarding to control, with an obvious decreases on its yield upon further doses. Practically, CGL activity from the solid A. carneus cultures, using rice bran as substrate, was increased by 1.2-fold, comparing to submerged cultures, under optimum conditions.

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“…When RAPD-PCR is used for classification and typing, the patterns obtained from amplification with two or three different primers are usually combined (3,7,8,21,23,28,30,31), and the procedures for reading the patterns, calculating similarity or distance measures, and clustering the patterns are time-consuming and cumbersome when performed by human operators or require expensive instrumentation or software. In order to develop a rapid, inexpensive, robust, and reliable method for identification at the species level of S. thermophilus, E. faecium, and E. faecalis, we compared three multivariate statistical techniques (cluster analysis, LDA, and CT) with ANNs for the analysis of RAPD-PCR patterns generated with a single primer (XD9).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Statistical Methods for Identification of Streptococcus thermophilus, Enterococcus faecalis, and Enterococcus faecium from Randomly Amplified Polymorphic DNA Patterns

Moschetti

Blaiotta

Villani

et al. 2001

Appl Environ Microbiol

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Thermophilic streptococci play an important role in the manufacture of many European cheeses, and a rapid and reliable method for their identification is needed. Randomly amplified polymorphic DNA (RAPD) PCR (RAPD-PCR) with two different primers coupled to hierarchical cluster analysis has proven to be a powerful tool for the classification and typing of Streptococcus thermophilus, Enterococcus faecium, and Enterococcus faecalis (G. Moschetti, G. Blaiotta, M. Aponte, P. Catzeddu, F. Villani, P. Deiana, and S. Coppola, J. Appl. Microbiol. 85:25-36, 1998). In order to develop a fast and inexpensive method for the identification of thermophilic streptococci, RAPD-PCR patterns were generated with a single primer (XD9), and the results were analyzed using artificial neural networks (Multilayer Perceptron, Radial Basis Function network, and Bayesian network) and multivariate statistical techniques (cluster analysis, linear discriminant analysis, and classification trees). Cluster analysis allowed the identification of S. thermophilus but not of enterococci. A Bayesian network proved to be more effective than a Multilayer Perceptron or a Radial Basis Function network for the identification of S. thermophilus, E. faecium, and E. faecalis using simplified RAPD-PCR patterns (obtained by summing the bands in selected areas of the patterns). The Bayesian network also significantly outperformed two multivariate statistical techniques (linear discriminant analysis and classification trees) and proved to be less sensitive to the size of the training set and more robust in the response to patterns belonging to unknown species.A large variety of genotypic and phenotypic methods are currently used for the identification and classification of microorganisms (32). Many of these techniques generate complex patterns whose interpretation for classification and identification purposes requires multivariate statistical techniques. Randomly amplified polymorphic DNA (RAPD) PCR (RAPD-PCR) is one of the most popular genotypic typing techniques. It was developed to reveal intra-and interspecific differences in bacterial genomes (33,35), and since it can be performed not only on purified DNA (35) but also on untreated (19) or lysed cells without DNA extraction (23), it can replace time-consuming restriction endonuclease analysis in strain typing and DNA-DNA hybridization techniques for species identification. In fact, RAPD-PCR has been used for the classification of a variety of food-borne microorganisms, including Saccharomyces spp. Statistical treatment of RAPD-PCR patterns usually involves calculation of a similarity matrix and use of hierarchical cluster analysis for grouping of the patterns. Similarity can be calculated using the formula of Nei and Li (24) when only the presence or the absence of bands is scored (21, 23), while Pearson's product-moment correlation coefficient is used when both the position and the intensity of bands are measured with image analysis software (3,28,31). Although unknown isolates can be assigned to a speci...

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Molecular tools for identification of Penicillium starter cultures used in the food industry

Cited by 46 publications

References 25 publications

Morphological and molecular differentiation of the pectinase producing fungi Penicillium expansum and Penicillium griseoroseum

Morphological and molecular differentiation of the pectinase producing fungi Penicillium expansum and Penicillium griseoroseum

Screening, morphological and molecular characterization of fungi producing cystathionine γ-lyase

Comparison of Statistical Methods for Identification of Streptococcus thermophilus, Enterococcus faecalis, and Enterococcus faecium from Randomly Amplified Polymorphic DNA Patterns

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