Molecular typing of canine parvovirus strains circulating from 2008 to 2012 in an organized kennel in India reveals the possibility of vaccination failure

Mittal, Mitesh; Chakravarti, Soumendu; Mohapatra, Jajati K.; Chug, P.K.; Dubey, Rahul; Upmanuyu, Vikramaditya; Narwal, Partap Singh; Kumar, Anil; Churamani, C.P.; Kanwar, Neena

doi:10.1016/j.meegid.2014.01.015

Cited by 69 publications

(61 citation statements)

References 36 publications

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“…In parallel to the previous reports of vaccination failure [11], 17.4% of infected dogs in the current study were vaccinated against CPV-2. PCR positivity in recently vaccinated dogs may be due to the continuous excretion of the vaccine strain through feces up to 2-3 weeks following vaccination.…”

Section: Discussionsupporting

confidence: 86%

Polymerase chain reaction based epidemiological investigation of canine parvoviral disease in dogs at Bareilly region

Thomas¹,

Singh²,

Goswami³

et al. 2014

Vet World

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. How to cite this article: Thomas J, Singh M, Goswami TK, Verma S, Badasara SK (2014). Polymerase chain reaction based epidemiological investigation of canine parvoviral disease in dogs at Bareilly region, Veterinary World 7(11): 929-932. AbstractAim: The aim of this study was to screen the suspected samples by polymerase chain reaction (PCR) and epidemiological analysis of positive cases of canine parvovirus type2. Materials and Methods:Fecal samples were collected from dogs suspected for canine parvovirus type 2 (CPV-2) and viral DNA was extracted. Primers were designed, and PCR was done with all extracted DNA samples. Age, sex and breed wise distribution of positive cases were analyzed.Results: Out of a total 44 collected fecal samples, 23 were found to be positive for CPV-2 by developed PCR. The disease was found to be more common in Labrador male pups of 3-6 months of age. The percentage of positive cases in vaccinated dogs was found to be around 17.4%. Conclusion:Almost half (52.3%) of total collected samples were found to be positive by PCR. However, number of field samples are needed to further validate this test and additionally sequence analysis needs to be done to ensure the prevalent field strain of CPV-2.

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Section: Discussionsupporting

confidence: 86%

Polymerase chain reaction based epidemiological investigation of canine parvoviral disease in dogs at Bareilly region

Thomas¹,

Singh²,

Goswami³

et al. 2014

Vet World

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“…Similar codon changes at amino acid positions 324 were also reported in the CPVs isolated in different countries [28,41,[44][45][46]. Horiuchi et al [47] revealed that the residue 324 was prone to strong positive selection in all carnivorous CPV isolates.…”

Section: Discussionsupporting

confidence: 62%

“…This residue was undergoing positive selection for the past few years and had evolved independently in different populations, which explained its world-wide mutation in unrelated CPV-2 populations [51]. Similar codon change at amino acid position 440 was also reported in CPVs isolated in different countries [28,44,52]. Mutation at amino acid residue 440 would, therefore, result in emergence of further antigenic variants.…”

Section: Discussionmentioning

confidence: 56%

“…The amino acid residue 267 is not exposed on the capsid surface and substitutions in this position may not affect the antigenicity of the virus [42]. All the CPV types analyzed in this study also showed amino acid change Ser297Ala, which was under strong positive selection pressure and played important role in host cell adaption [43,44].…”

Section: Discussionmentioning

confidence: 83%

“…It was also documented that Ile-324 substitution was in regions of potentially important antigenic epitopes and this substitution might have a direct influence on viral biology [48]. Interestingly, the frequency of CPVs carrying mutations at 267Tyr and 324Ile residues had increased enormously and had reached a high prevalence among Indian CPV isolates only since 2012-13 [27,28,44].…”

Section: Discussionmentioning

confidence: 99%

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Full-length VP2 gene analysis of canine parvovirus reveals emergence of newer variants in India

Nookala¹,

Mukhopadhyay²,

Sivaprakasam³

et al. 2016

Acta Microbiologica et Immunologica Hungarica

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The canine parvovirus (CPV) infection is a highly contagious and serious enteric disease of dogs with high fatality rate. The present study was taken up to characterize the full-length viral polypeptide 2 (VP2) gene of CPV of Indian origin along with the commercially available vaccines. The faecal samples from parvovirus suspected dogs were collected from various states of India for screening by PCR assay and 66.29% of samples were found positive. Six CPV-2a, three CPV-2b, and one CPV-2c types were identified by sequence analysis. Several unique and existing mutations have been noticed in CPV types analyzed indicating emergence of newer variants of CPV in India. The phylogenetic analysis revealed that all the field CPV types were grouped in different subclades within two main clades, but away from the commercial vaccine strains. CPV-2b and CPV-2c types with unique mutations were found to be establishing in India apart from the prevailing CPV-2a type. Mutations and the positive selection of the mutants were found to be the major mechanism of emergence and evolution of parvovirus. Therefore, the incorporation of local strain in the vaccine formulation may be considered for effective control of CPV infections in India.

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Canine Parvovirus

Singh¹,

Chander²,

Nandi³

2019

Recent Advances in Animal Virology

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Molecular typing of canine parvovirus strains circulating from 2008 to 2012 in an organized kennel in India reveals the possibility of vaccination failure

Cited by 69 publications

References 36 publications

Polymerase chain reaction based epidemiological investigation of canine parvoviral disease in dogs at Bareilly region

Polymerase chain reaction based epidemiological investigation of canine parvoviral disease in dogs at Bareilly region

Full-length VP2 gene analysis of canine parvovirus reveals emergence of newer variants in India

Canine Parvovirus

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