Molecularly imprinted polymer-based, solid-phase extraction followed by liquid chromatographic determination of lacosamide in rat plasma

Rao, R. Nageswara; Sravan, B.; Reddy, Ch. Krishna; Meena, Sunita; Prashamsa, J.; Spoorthy, P.

doi:10.1039/c3ay42186d

Cited by 3 publications

(3 citation statements)

References 18 publications

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“…It can be seen from Fig. that, it is consistent in pepper oil resin and after purification with MIP, there are three peaks present in the HPLC chromatograph, which are typical in the analysis of acid amide or numb‐taste compounds in prickly ashes . However, two peaks are present in the effluent of NIP‐SPEC.…”

Section: Resultsmentioning

confidence: 99%

Preparation and characterization of molecularly‐imprinted polymers for extraction of sanshool acid amide compounds followed by their separation from pepper oil resin derived from Chinese prickly ash (Zanthoxylum bungeanum)

Chen

Jin

Yao

et al. 2017

J of Separation Science

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Molecularly imprinted polymers were prepared using the molecular structure analogs of sanshool as template molecule, 2-vinylpyridine and β-cyclodextrin as double functional monomers, ethylene dimethacrylate as cross linker, and azobisisobutyronitrile as initiator. The structural characteristics of the polymers were determined by Fourier-transform infrared spectroscopy and scanning electron microscopy. Dynamic adsorption and isothermal adsorption were also investigated. The molecularly imprinted polymers were used to prepare a molecularly imprinted solid-phase extraction column in order to separate acid amide components from pepper oil resin derived from Chinese prickly ash (Zanthoxylum bungeanum). After eluting, the percentage of acid amide components was enhanced to 92.40 ± 1.41% compared with 23.34 ± 1.21% in the initial pepper oil resin, indicating good properties of purification of molecularly imprinted polymers and potential industrial application.

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Section: Resultsmentioning

confidence: 99%

Preparation and characterization of molecularly‐imprinted polymers for extraction of sanshool acid amide compounds followed by their separation from pepper oil resin derived from Chinese prickly ash (Zanthoxylum bungeanum)

Chen

Jin

Yao

et al. 2017

J of Separation Science

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“…According to literature, only a few methods were reported for the determination of LCA in biological samples using HPLC [7], GC–MS [8], and LC–MS/MS [9–12]. Greenaway [7] et al proposed an HPLC method and Nikolaou et al developed a GC‐MS [8] method for determination of LCA in epileptic patients as well as in spiked human plasma, however, these methods have not used deuterated compound as Internal standard, which cannot eliminate the variability in results, reproducibility and moreover the run time also high.…”

Section: Introductionmentioning

confidence: 99%

“…Greenaway [7] et al proposed an HPLC method and Nikolaou et al developed a GC‐MS [8] method for determination of LCA in epileptic patients as well as in spiked human plasma, however, these methods have not used deuterated compound as Internal standard, which cannot eliminate the variability in results, reproducibility and moreover the run time also high. Kim [9] et al., Rao [10] et al., and Shah [11] et al. reported methods for the determination of LCA in rat biological fluids, but these methods cannot be applied for a clinical study.…”

Section: Introductionmentioning

confidence: 99%

Determination of lacosamide in human plasma by LC–MS/MS and its application to a human pharmacokinetic study

Yadlapalli

Katari

Surya

2020

Separation Science Plus

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A highly sensitive, specific, and rapid liquid chromatography–tandem mass spectrometry method for determination of lacosamide in human plasma has been developed using lacosamide d6 as an internal standard with 50 µL of human plasma. Protein precipitation technique was used to extract the analyte from human plasma. The chromatographic separation was achieved on ZODIAC C18, 4.6 × 100 mm, 5 µm column using an isocratic mobile phase composition of acetonitrile and 0.1% formic acid buffer (80:20, v/v) at a flow rate of 0.9 mL/min. A detailed method validation was performed as per the recent US FDA guidelines and the calibration curve obtained was linear (r2 = 998) over the concentration range 0.075–25.461 µg/mL. API‐4500 liquid chromatography with tandem mass spectrometry was operated with multiple reaction monitoring mode during the entire analysis. The proposed method was successfully applied to a pharmacokinetic study in healthy, adult human volunteers after oral administration of lacosamide 200 mg film‐coated tablet under fed conditions.

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Molecularly Imprinted Polymers as Biomimetic Molecules: Synthesis and their Pharmaceutical Applications

Rezapour¹,

Faridbod

Norouzi

2015

Handbook of Polymers for Pharmaceutical Technologies

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Molecularly imprinted polymer-based, solid-phase extraction followed by liquid chromatographic determination of lacosamide in rat plasma

Cited by 3 publications

References 18 publications

Preparation and characterization of molecularly‐imprinted polymers for extraction of sanshool acid amide compounds followed by their separation from pepper oil resin derived from Chinese prickly ash (Zanthoxylum bungeanum)

Preparation and characterization of molecularly‐imprinted polymers for extraction of sanshool acid amide compounds followed by their separation from pepper oil resin derived from Chinese prickly ash (Zanthoxylum bungeanum)

Determination of lacosamide in human plasma by LC–MS/MS and its application to a human pharmacokinetic study

Molecularly Imprinted Polymers as Biomimetic Molecules: Synthesis and their Pharmaceutical Applications

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