Monoclonal Xenogeneic Antibodies to Mouse Leukocyte Antigens: Identification of Macrophage-Specific and Other Differentiation Antigens

Springer, Timothy A.; Galfrè, G.; Secher, David S.; Milstein, C

doi:10.1007/978-3-642-67448-8_7

Cited by 11 publications

(6 citation statements)

References 11 publications

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“…wt. protein antigen identified by M1/9.3 is a leukocyte-common antigen (LCA) found on essentially all leukocytes but not on nonlymphoid tissue [3,4].…”

Section: Introductionmentioning

confidence: 99%

Mac‐1: a macrophage differentiation antigen identified by monoclonal antibody

et al. 1979

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We have previously described the derivation of M1/70, a hybrid myeloma line secreting monoclonal rat anti‐mouse cell surface antibody (Springer, T., Galfre, G., Secher, D. S. and Milstein, C, Eur. J. Immunol. 1978. 8: 539). We have now investigated the cellular distribution of this antigen using a 125I‐labeled anti‐rat IgG indirect binding assay, the fluorescence‐activated cell sorter, autoradiography and precipitation of cell surface molecules. Screening with a tumor cell panel showed strong reactivity with a macrophage‐like line but no reactivity with B or T lymphoma lines. In normal tissues, M1/70 antigen was found to be present in small amounts on spleen and exudate granulocytes and a subpopulation of bone marrow cells, in moderate amounts on spleen and blood monocytes and expressed in much larger amounts on spleen histiocytes and peritoneal exudate macrophages. In contrast, M1/70 antigen was found to be absent from erythroid and lymphoid cells. M1/70 antibody precipitated two polypeptides of 190 000 and 105 000 mol. wt. which were present in much greater amounts on peritoneal exudate macrophages than on spleen cells. The expression on phagocytes of two other antigens identified by monoclonal antibodies M1/69 and M1/9.3 was also examined. Monocytes and granulocytes expressed large amounts of M1/69 and low amounts of M1/70 antigen, while in peritoneal exudate macrophages this pattern was dramatically reversed. M1/70 thus defines a differentiation antigen on mononuclear phagocytes and granulocytes, the expression of which is specifically increased during monocyte maturation. This antibody is the first to be described which recognizes a discrete molecule specific to phagocytes.

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“…wt. protein antigen identified by M1/9.3 is a leukocyte-common antigen (LCA) found on essentially all leukocytes but not on nonlymphoid tissue [3,4].…”

Section: Introductionmentioning

confidence: 99%

Mac‐1: a macrophage differentiation antigen identified by monoclonal antibody

et al. 1979

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“…LAK cell cultures are phenotypically heterogeneous and comprise NK cells, CD8+ T cells, and B cells [19,25]. CD4+ T lymphocytes, on the other hand, are virtually absent from these cultures [7,21].…”

Section: Discussionmentioning

confidence: 99%

“…The rat anti-mouse' mAb CZ-1 (IgM, x ) was generated in our laboratory as described in detail previously [9]. The following anti-CD45 exon-specific mAb were used: the exon A-specific 14.8 [17] and the exon C-specific DNL 1.9 [18] mAb were used as ammonium sulfate-cut cell culture supernatants and provided by Dr. David Parker, UMMC; the B exon-specific CD45R-1 (clone 16A; [13]), either unlabeled or conjugated to RED613 (GibcoBRL, Gaithersburg, MD); and the framework-specific CD45 clone M19.3.HL.2 [19], pan-T200, unlabeled or directly conjugated with FITC (Gibco). Mouse IFN-fi (800 KU) was obtained from Lee Biomolecular (San Diego, CA).…”

Section: Antibodies and Reagentsmentioning

confidence: 99%

The monoclonal antibody CZ‐1 identifies a mouse CD45‐associated epitope expressed on interleukin‐2‐responsive cells

et al. 1993

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We have previously described a monoclonal antibody (mAb), CZ-1, which reacts with an epitope expressed on most peripheral basophils, natural killer cells, B cells, and CD8+ T cells, but not with most thymocytes or peripheral CD4+ T cells. Here we show that mAb CZ-1 defines a sialic acid-dependent epitope associated with a subpopulation of CD45 molecules. This conclusion is based on the ability to block binding of mAb CZ-1 by sialic acid, neuramin-lactose, neuraminidase, and mAb to CD45RB, and by expression of the epitope on transfected psi 2 cells expressing exon B of CD45. The results suggest that the CZ-1 epitope is a post-translational modification expressed on a subpopulation of the CD45 molecules also expressing the B exon. Expression of the CZ-1 epitope was required for freshly isolated lymphocytes to respond to interleukin-2 (IL-2). Depletion of CZ-1+ cells by C' or by cell sorting of thymocytes or splenocytes eliminated the IL-2 responsive cells. The subpopulations of thymocytes and CD4+ splenocytes responding to IL-2 were exclusively within the small CZ-1+ subpopulation. mAb CZ-1 was also used to subdivide CD45+ and CD45RB+ splenocytes into IL-2-responsive and -nonresponsive subpopulations. The CZ-1 epitope was also expressed on virtually all lymphokine-activated killer cell precursors. These data, thus, indicate that cells responsive to IL-2 express this sialated modification of CD45.

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“…Decrease in intraperitoneal TNF activity induced by the injection of MM46 carcinoma cells and BPV by antimacrophage antibody Decrease in intraperitoneal TNF activity by anti-macrophage Ab which recognises a Mac-I antigen on mononuclear phagocytes (Springer et al, 1979) is shown in Table IV. TNF activity was induced in the peritoneal fluid by the i.p.…”

Section: Cell Linementioning

confidence: 99%

Intratumoral induction of tumour necrosis factor by systemic administration of Bordetella pertussis vaccine

Minagawa

Katori²,

Yoshida³

et al. 1990

Br J Cancer

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Summary Intratumoral induction of tumour necrosis factor (TNF) by administration of Bordetella pertussis vaccine (BPV) as compared with that by the agent OK-432 was investigated in mice. Two hours after such administration tumour tissues tested were resected from the mice, homogenised, and the TNF activities in the homogenate were assayed using a L-929 fibroblast assay. Intravenous injection of BPV into mice bearing the MM46 carcinoma resulted in a greater concentration of TNF in the tumour homogenate than in the serum. With OK-432, however, there was a greater concentration of TNF in the serum than in the tumour homogenates. A high level of intratumoral TNF induction by BPV was also observed in mice bearing Meth A fibrosarcoma or Lewis lung carcinoma. The therapeutic effect against the Meth A fibrosarcoma was in parallel with the intratumoral TNF activity. Intratumoral TNF activity is therefore believed to be a good index of therapeutic effect.

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Monoclonal Xenogeneic Antibodies to Mouse Leukocyte Antigens: Identification of Macrophage-Specific and Other Differentiation Antigens

Cited by 11 publications

References 11 publications

Mac‐1: a macrophage differentiation antigen identified by monoclonal antibody

Mac‐1: a macrophage differentiation antigen identified by monoclonal antibody

The monoclonal antibody CZ‐1 identifies a mouse CD45‐associated epitope expressed on interleukin‐2‐responsive cells

Intratumoral induction of tumour necrosis factor by systemic administration of Bordetella pertussis vaccine

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