Morphological and Immunohistochemical Characteristics of a
Cell Line Originated from Noninvasive Human Bladder
Transitional-Cell Carcinoma

Logothetou-Rella, H; Vamvassakis, E.; Nesland, J. M.; J, Hadjiminas; Dimopoulos, C.

doi:10.1159/000472900

Cited by 7 publications

(8 citation statements)

References 11 publications

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“…The ultrastructural features were the same as de scribed in an earlier report [2], The irregular nuclei had evenly distributed chromatin and one or several promi nent nucleoli. Abundant, partly dilated rough endoplas mic reticulum ( fig.…”

Section: Electron Microscopysupporting

confidence: 70%

“…The biopsy specimens were sliced and dispersed enzymatically (trypsin-collagenase) and primary cultures were established and cul tivated as described previously [2], Primary cells were seeded in Petri dishes, grown in RPMI-1640, supplemented with 15% FCS and incubated at 37 °C. The growth medium was changed every 3 days.…”

Section: Introductionmentioning

confidence: 99%

“…We have recently shown that urothelial carcinomas in monolayer culture chiefly exhibit four phenotypic ex pressions, among which are cells that contain large amounts of glycosaminoglycans, which in turn are re sponsible for tumor-nodule formation [2], Therefore, in order to evaluate the importance of this phenomenon, cells from primary urothelial carcinomas were cultured and studied by light and electron microscopy.…”

Section: Introductionmentioning

confidence: 99%

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Common Characteristics of Primary Cell Cultures Originated from Invasive Urothelial Carcinoma

Logothetou-Rella¹,

Jm²,

Vamvassakis³

et al. 1988

Eur Urol

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Section: Electron Microscopysupporting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Common Characteristics of Primary Cell Cultures Originated from Invasive Urothelial Carcinoma

Logothetou-Rella¹,

Jm²,

Vamvassakis³

et al. 1988

Eur Urol

Self Cite

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“…The biopsy was disaggregated enzymatically (trypsin-collagenase) and cultivated as described previously [4].…”

Section: Tissue Culturementioning

confidence: 99%

“…In these instances most of the procedures have in volved the use of outgrowths of epithelial fragments of bladder epithelium placed in culture medium. When applied to human bladder epithelium in our laboratory, these methods produced short-term cultures with poor plating efficiency upon subcultivation [4], We know that distinct structural differences exist between urothelial cells of patients with bladder cancer and normal individuals. However, interpretation of cy tologic specimens is sometimes difficult and rather sub jective.…”

Section: Cytogeneticsmentioning

confidence: 99%

Growth Characteristics of Cultured Human Normal Bladder Epithelial Cells: A Comparison with Urothelial Carcinoma Cell Cultures

Logothetou-Rella¹,

Nesland²,

Karayiannis³

et al. 1988

Eur Urol

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Culture of a prostatic cell line in basement membrane gels results in an enhancement of malignant properties and constitutive alterations in gene expression

Freeman

Bägli

Lamb

et al. 1994

Journal Cellular Physiology

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Interaction of a transformed rat prostate epithelial cell (NbMC-2) with basement membrane gels (Matrigel) has been evaluated using a long-term matrix culture system. NbMC-2 cells, and single-cell clonal derivatives, formed spheroidal multicellular structures (aggregates) on Matrigel surfaces and were weakly invasive or noninvasive during a 1 week period. During subsequent 2-4 week periods, invasive cells originating from the aggregates and exhibiting a fusiform morphology became evident and increased in number in the matrix cultures. This biphasic pattern of behavior did not occur on laminin, type I or type IV collagen, or fibronectin substrates, but it did occur on Matrigel in serum-free medium. Characterization of sublines enriched in fusiform cells indicated that they maintained their distinct morphology with continuous culture. Further, they exhibited significantly greater invasive potential, saturation density, and random motility (chemokinesis) than the parent cell line. Steady-state levels of fibronectin mRNA were highly elevated in the fusiform variants, demonstrating a constitutive alteration in patterns of gene expression coinciding with the altered morphology. These results indicate that clonal NbMC-2 cells differentiate at a reproducible frequency into a more aggressive cell type in response to culture in the basement membrane-like matrix. The altered phenotypic properties appear to be stable since they can be inherited by daughter cells and because they are evident in the absence of matrix. These observations suggest a cell-specific mechanism for promotion of malignant growth by matrix-mediated induction of novel cell properties.

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Morphological and Immunohistochemical Characteristics of a Cell Line Originated from Noninvasive Human Bladder Transitional-Cell Carcinoma

Cited by 7 publications

References 11 publications

Common Characteristics of Primary Cell Cultures Originated from Invasive Urothelial Carcinoma

Common Characteristics of Primary Cell Cultures Originated from Invasive Urothelial Carcinoma

Growth Characteristics of Cultured Human Normal Bladder Epithelial Cells: A Comparison with Urothelial Carcinoma Cell Cultures

Culture of a prostatic cell line in basement membrane gels results in an enhancement of malignant properties and constitutive alterations in gene expression

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