Morphological characterization of Coelogyne spp for germplasm conservation of orchids

Hartati, Sri; Muliawati, Endang Setia; Pardono, Pardono; Cahyono, Ongko; Yuliyanto, Ponco

doi:10.1590/0034-737x201966040004

Cited by 18 publications

(14 citation statements)

References 15 publications

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“…(De et al 2015b;Darmawati et al 2018;Indraloka et al 2019), Coelogyne spp. (Hartati et al 2019a); Phaius spp. (Devadas et al, 2019;Hartati et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Morphological characteristics of Phaius spp. orchids from Indonesia

et al. 2021

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Abstract. Hartati S, Samanhudi, Manurung IR, Cahyono O. 2021. Morphological characteristics of Phaius spp. orchids from Indonesia. Biodiversitas 22: 1991-1995. Orchid is one of the ornamental plants with great economic value in various communities. Characterization and grouping of several orchid species can be used in breeding efforts to obtain hybrid with the desired traits. This study is aimed to characterize the morphological appearance of Phaius orchids and to cluster the species based on their similarity in morphological characteristics. Five species of Phaius (P. tankervilleae, P. indigoferus, P. montanus, P. amboinensis, and P. callosus) of the collection of the Bogor Botanical Garden were observed based on 30 morphological characters including the characters of stems, pseudobulbs, leaves and flowers. The data obtained were converted into binary data and computed using the Numerical Taxonomy and Multivariate Analysis System (NTSYS) Spc 2.02 program to produce dendrogram. The results show that based on the morphological characterization of the five species of Phaius spp., they have similarities from 63% to 80%. Among the five Phaius species, P. amboinensis, P. tankervilleae, and P. montanus had 80% similarity and can be used as parents for crossing.

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“…(De et al 2015b;Darmawati et al 2018;Indraloka et al 2019), Coelogyne spp. (Hartati et al 2019a); Phaius spp. (Devadas et al, 2019;Hartati et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Morphological characteristics of Phaius spp. orchids from Indonesia

et al. 2021

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“…Additionally, cytological studies have shown that the karyotype of C. pandurata is 2n=56, C. rumphii is 2n=72 and their hybrid is 2n=54 (Hartati et al 2017). A second study showed that based on the morphological identification of these two species, they have a morphological similarity of 87% (Hartati et al 2019a). Furthermore, the crosses of C. pandurata and C. rumphii are 100% successful using selfing, crossing, and reciprocal methods (Hartati et al 2019b).…”

Section: Introductionmentioning

confidence: 99%

Short Communication: Genetic variation of Coelogyne pandurate, C. rumphii and their hybrids based on RAPD markers

Hartati

Muliawati

2020

Biodiversitas

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Abstract. Hartati S, Muliawati ES. 2020. Short Communication: Genetic variation of Coelogyne pandurata, C. rumphii and their hybrids based on RAPD markers. Biodiversitas 21: 4709-4713. One effort to increase the genetic diversity of orchids is by crossing. This research aims to assess the genetic variation of a hybrid orchid obtained by crossing Coelogyne pandurata and C. rumphii and their hybrids based on RAPD markers. In this research, both parents were analyzed in three replications, while the hybrid was done in 10 replications. The study was conducted by analyzing DNA bands using RAPD markers with six primers, i.e. OPA 02, OPA 07, OPA 13, OPB 12, OPB 17, and OPD 08. Identification of the parents and their F1 hybrids showed 95.83% polymorphic bands with 43 bands measuring 200-2100 bp. The parents of C. rumphii, C. pandurata, and their hybrids showed similarity range of 0.16-1.00. The crossing of C. rumphii and C. pandurata resulted in a similarity of 0.5, shown in two large clusters. The first cluster consisted only of C. pandurata males and the second cluster consisted of C. rumphii females, together with all hybrid individuals. This study succeeded in creating new hybrids of orchids that have different characters from their parents, having a genetic variation of 23%.

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“…Our results are similar to those of Mat-Yasin et al [14], who investigated the propagation medium of Spathoglottis plicata. Hartati et al [15] found that the addition of NAA and coconut water to medium for in vitro black orchid propagation has increasing number of roots. However, at physiological concentrations, cytokinins activate the shoot apical meristem and shoot growth but suppress the root apical meristem and root growth [16] Cytokinins promote cell division, stimulate the release of lateral buds from apical dominance, delay leaf senescence, and enhance chloroplast development.…”

Section: Resultsmentioning

confidence: 99%