Morphological defects of sperm and their association with motility, fertility, and hatchability in four Korean native chicken breeds

Abstract

ObjectiveThis study was conducted to compare morphological defects, viability, motility (MOT), fertility (F), and hatchability (H) in four Korean native chicken breeds (KNCBs), and to evaluate whether defective segments of spermatozoa are associated with MOT, F, and H.MethodsFour KNCBs, including Korean Ogye (KO), Hwangbong (HB), Hyunin Black (HH), and Hoengseong Yakdak (HY) were used. White Leghorn (WL) was used as a control. Nine cocks from each breed were randomly assigned into three groups. Semen was collected by abdominal massage method. Eosin-nigrosin staining method was used to identify live-dead spermatozoa. Different segments and specific morphological defects of spermatozoa were identified using 4′, 6-diamidino-2-phenylidole and MitoTracker Red CMXRos. F and H rates were evaluated following artificial insemination (AI).ResultsKO had the highest MOT rate compared to HY. Viable normal sperm rates of KO and HH were high and comparable with WL. HY spermatozoa had the highest viable abnormal sperm (VAS) or morphological defect rate followed by HB. Likewise, HB spermatozoa had the highest dead sperm (dead) rate compared to KO, HY, and WL. Bent, coiled, detached, broken, and knotted were common identified specific morphological defects for all breeds. Most morphological defects were at the head and tail in all breeds. VAS showed strong negative correlation with MOT (r = −0.697) and F (r = −0.609). Similarly, defective tail was negatively correlated with MOT (r = −0.587), F (r = −0.797), and H (r = −0.448). The F and H rates of KO and WL were comparable.ConclusionThese data indicate that most identified specific morphological defects are at the head and tail. VAS and defective tail were associated with poor motility, F, and H. KNCBs showed more morphological defects than WL. Finally, these results will facilitate successful AI and semen cryopreservation.

The sperm longevity and freezability in the modified BHSV extender of Thai Pradu-hangdum chicken

“…Semen was collected by the dorso-abdominal massage technique, and the longevity of sperm in extender at different time points was evaluated. The morphology of Pradu-hangdum chicken sperm was similar as with other breeds of chicken as illustrated in previous studies (Abu Md Mamun et al, 2013;Feyisa et al, 2018). The mean concentration of fresh semen in this study was higher than that of Arabic male chickens at 2.20 ± 0.37 billion sperm / mL (Almahdi et al, 2014).…”

Cryopreservation of Indonesian native chicken semen by using dimethyl sulfoxide and various level of ethylene glycol as cryoprotectants

“…Bent head, midpiece, and tail were the main morphological abnormalities found in this study. According to Feyisa et al (2018), there was a difference in the proportion of spermatozoa with defective segments among breeds. The results of research by Ameen et al (2014) stated that sperm abnormalities in the Hubbard and Isa White strains were dominated by head abnormalities.…”

Interaction Effect of Vitamin E-selenium Supplementation and Metabolic Energy on Reproductive Performance of Joper Breeders

“…abnormal spermatozoa and dead spermatozoa. Good quality spermatozoa if spermatozoa abnormality <17% and dead spermatozoa <10% (Feyisa et al 2018) Spermatozoa concentration plays an important role in the success of artificial insemination. In this study, the concentration of spermatozoa used for each treatment was the same, namely 100 million spermatozoa/hens.…”