Moxibustion regulates inflammatory mediators and colonic mucosal barrier in ulcerative colitis rats

Ma, Tao; Xu, Nuo; Ma, Xiande; Bai, Zeng-Hua; Xing, Tao; Yan, Hong-Chi

doi:10.3748/wjg.v22.i8.2566

Cited by 21 publications

(14 citation statements)

References 27 publications

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“…We showed that HPM significantly downregulated the expression of IL-1β, IL-1R6, Fas and FasL, which agreed with previous studies[ 16 , 34 - 40 ]. We demonstrated that inflammatory infiltration and apoptosis in the colonic epithelium, controlled by cytokines and their network, are crucial factors in the pathogenesis of UC.…”

Section: Discussionsupporting

confidence: 93%

Herb-partitioned moxibustion alleviates colon injuries in ulcerative colitis rats

Zhang

Ren

Wei

et al. 2018

WJG

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AIMTo observe the effect of herb-partitioned moxibustion (HPM) on expression of colonic cytokines in ulcerative colitis (UC) rats.METHODSA UC rat model was established by protein immunization in combination with topical chemical stimulation. Rats in the HPM group (n = 8) received HPM at bilateral Tianshu (ST25) points. The gross injury and pathological scores of the colon were recorded. The expression profile of colonic cytokines was assayed using the protein microarray technique. Specific differential cytokines were selected and verified by ELISA. The corresponding UniProt Accessions of the differentially expressed cytokines were retrieved in the UniProt database. The pathways involved were analyzed with the help of the KEGG PATHWAY database. The DAVID database was used for functional cluster and pathway analysis.RESULTSHPM improved colon injuries in UC rats, manifested by accelerated repair of ulcers and alleviation of inflammation, and the gross injury and pathological scores both significantly decreased (P < 0.01). Fold change > 1.3 or < 0.77 was taken as the screening standard. There were 77 down-regulated and 9 up-regulated differentially expressed colonic cytokines in the HPM group compared with the model group, and expression of 20 differed significantly (P < 0.05). Twelve of the 20 significantly differentially expressed cytokines [β-catenin, interleukin-1 receptor 6 (IL-1R6), IL-1β, B7-1, nerve growth factor receptor, AMP-activated protein kinase-α1, neuropilin-2, orexin A, adipocyte differentiation-related protein, IL-2, Fas and FasL] were up-regulated in the model group (n = 3, compared with the normal group) but down-regulated in the HPM group (n = 3, compared with the model group). Functional cluster analysis showed that the differentially expressed colonic cytokines in the HPM group regulated apoptosis and protein phosphorylation. KEGG pathway analysis showed that 52 down-regulated and 7 up-regulated differentially expressed colonic cytokines in the HPM group had pathways. The pathways that interacted between the cytokines and their receptors accounted for the largest proportion (28 of the down-regulated and 5 of the up-regulated cytokines).CONCLUSIONHPM promotes the repair of colon injuries in UC rats, which is related to the regulation of several abnormally expressed cytokines.

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Section: Discussionsupporting

confidence: 93%

Herb-partitioned moxibustion alleviates colon injuries in ulcerative colitis rats

Zhang

Ren

Wei

et al. 2018

WJG

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“…Several studies have shown that EA and moxibustion can regulate inflammatory mediators and colonic mucosal barrier in UC model rats. For instance, Ma et al found that moxibustion at CV4 and ST36 acupoints could improve intestinal mucosal tissue repair by reducing TNF-α and p38MAPK and increasing the expression of occludin and ZO-1 in colon tissues [47]. Also, EA at ST36 point could regulate the intestinal immune function by modulating IL-1β and nAchRα7 mRNA and reduce mucosal lesions to achieve the purpose of treating UC [48].…”

Section: Discussionmentioning

confidence: 99%

Electroacupuncture and Moxibustion Regulate Hippocampus Glia and Mitochondria Activation in DSS‐Induced Colitis Mice

Zhang

Xie

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Objectives. To study the influence of electroacupuncture (EA) and moxibustion on the hippocampus astrocyte and microglia activation in the ulcerative colitis model and to evaluate the mitochondria activity. Methods. 2.5% dextran sodium sulfate-induced colitis mice were treated by EA or moxibustion. Intestinal pathological structure was observed by hematoxylin and eosin (H&E) staining; the expression of GFAP or S100b (markers for astrocyte), Iba-1 (a marker for microglia), and Mitofilin (a marker for mitochondria) in hippocampus was detected by immunofluorescence staining or western blot. Results. The results demonstrated that both EA and moxibustion could improve the morphology of distal colonic mucosal epithelia in DSS-induced colitis mice. Expression of GFAP in the hippocampus was significantly increased after EA or moxibustion treatment. The effects were further supported by WB results. Meanwhile, expression of mitofilin in the hippocampus CA1 and CA3 regions showed the same trend as that of GFAP. Expression of Iba-1 in the hippocampus showed no significant difference after EA or moxibustion treatment, while the state of microglia changed from resting in control mice to activated state in colitis mice. Conclusion. EA and moxibustion were able to modulate the activation of astrocyte, microglial, and mitochondria in the hippocampus area in the colitis model.

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“…According to the equivalent dose-ratio table of human and animal body surface area: the dosage of the rats = X mg/kg × 70 kg × 0.018/0.2 kg (X is the adult clinical dosage). 23,24 The normal control (no handling) rats were classified as the NH group, NMS + RS rats were divided into 5 groups: NMS + RS; low dose group: FLWCK 1.125 mg/100 g; middle dose group: FLWCK 2.25 mg/100 g; high dose group: FLWCK 4.5 mg/100 g; and the positive control group: pinaverium bromide 1.5 mg/100 g. From postnatal day 60, the rats in the FLCWK group and positive control group were given intragastric administration with treatment, respectively; the NH group and NMS + RS group were given distilled water. All groups were treated for 14 consecutive days.…”

Section: Drugs and Administrationmentioning

confidence: 99%

Effects of Fengliao-Changweikang in Diarrhea-predominant Irritable Bowel Syndrome Rats and Its Mechanism Involving Colonic Motility

Jia

Wang

et al. 2018

J Neurogastroenterol Motil

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Background/AimsThis study was designed to investigate the effect of Fengliao-Changweikang (FLCWK) in diarrhea-predominant irritable bowel syndrome (IBS-D) rats and explore its underlying mechanisms.MethodsIBS-D model rats were induced by neonatal maternal separation (NMS) combined with restraint stress (RS). In in vivo experiments, the model rats were randomly divided into 5 groups: NMS + RS, FLCWK (low dose, middle dose, and high dose), and pinaverium bromide. The normal control (no handling) rats were classified as the NH group. The therapeutic effect of FLCWK was evaluated by fecal characteristics, electromyographic response and abdominal withdrawal reflex scores. In in vitro experiments, the model rats were randomly divided into 2 groups: NMS + RS, FLCWK (middle dose), and no handling rats were used as the NH group. The differences in basic tension and ACh-induced tension of isolated colonic longitudinal smooth muscle strips (CLSMs) among the 3 groups were observed. In addition, different inhibitors (nifedipine, TMB-8, L-NAME, methylene blue, and 4-AP) were pretreated to explore the underlying mechanisms.ResultsIn in vivo experiments, fecal characteristics, electromyographic response, and abdominal withdrawal reflex scores significantly improved in the FLCWK group, compared with the NMS + RS group. In in vitro experiments, the basic tension and ACh-induced tension of CLSMs in IBS-D rats were significantly inhibited by FLCWK. After pre-treatment with different inhibitors, the ACh-induced tension of CLSMs in each group showed no significant difference.ConclusionsFLCWK manifested curative effect in IBS-D rats by inhibiting colonic contraction. The underlying mechanisms may be related to regulatory pathway of nitric oxide/cGMP/Ca2+ and specific potassium channels.

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Moxibustion regulates inflammatory mediators and colonic mucosal barrier in ulcerative colitis rats

Abstract: Reduction of TNF-α and p38MAPK and increased expression of occludin and ZO-1 in colonic tissue represent a potential mechanism for improved intestinal mucosal tissue repair with grain-sized moxibustion.

Cited by 21 publications

References 27 publications

Herb-partitioned moxibustion alleviates colon injuries in ulcerative colitis rats

Herb-partitioned moxibustion alleviates colon injuries in ulcerative colitis rats

Electroacupuncture and Moxibustion Regulate Hippocampus Glia and Mitochondria Activation in DSS‐Induced Colitis Mice

Effects of Fengliao-Changweikang in Diarrhea-predominant Irritable Bowel Syndrome Rats and Its Mechanism Involving Colonic Motility

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