2017
DOI: 10.1021/acs.jafc.7b02255
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Multi-imaging of Cytokinin and Abscisic Acid on the Roots of Rice (Oryza sativa) Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Abstract: Plant hormones act as important signaling molecules that regulate responses to abiotic stress as well as plant growth and development. Because their concentrations of hormones control the physiological responses in the target tissue, it is important to know the distributions and concentrations in the tissues. However, it is difficult to determine the hormone concentration on the plant tissue as a result of the limitations of conventional methods. Here, we report the first multi-imaging of two plant hormones, o… Show more

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Cited by 32 publications
(35 citation statements)
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“…SnRK2 then phosphorylates downstream proteins, including ABA‐responsive element (ABRE)‐binding transcription factors and ion channels. The distribution of ABA has successfully been detected by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF‐MS) imaging (Shiono et al ., 2017) and transcriptional biosensors (Wu et al ., 2018). Transcriptional biosensors were developed that consisted of synthetic promoters, based on repeats of the ABRE elements, upstream of GFP fused with an endoplasmic reticulum (ER)‐retention signal (erGFP) (Wu et al ., 2018).…”
Section: Plant Hormone Sensorsmentioning
confidence: 99%
“…SnRK2 then phosphorylates downstream proteins, including ABA‐responsive element (ABRE)‐binding transcription factors and ion channels. The distribution of ABA has successfully been detected by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF‐MS) imaging (Shiono et al ., 2017) and transcriptional biosensors (Wu et al ., 2018). Transcriptional biosensors were developed that consisted of synthetic promoters, based on repeats of the ABRE elements, upstream of GFP fused with an endoplasmic reticulum (ER)‐retention signal (erGFP) (Wu et al ., 2018).…”
Section: Plant Hormone Sensorsmentioning
confidence: 99%
“…Plant hormones are substances that regulate growth and development of plants. Cytokinin and abscisic acid are the plant hormones that have been visualized so far (Klein et al, 2015;Shiono et al, 2017). However, some of the plant hormones have not been visualized yet.…”
Section: Secondary Metabolites' Visualizationmentioning
confidence: 99%
“…Matrix-assisted laser desorption/ionization−imaging mass spectrometry (MALDI−IMS) is a powerful approach for visualizing the localization of metabolites and is used in sections of organisms (Dong et al 2016; Fujimura and Miura 2014; Lee et al 2012; Sarabia et al 2018; Sturtevant et al 2016). Recently, this approach has been applied to identify the localization of specialized metabolites in plants (Enomoto et al 2018; Jarvis et al 2017; Li et al 2014; Shiono et al 2017). One of the major challenges of MALDI−IMS is to keep plant tissue shapes during the preparation of sections.…”
Section: Introductionmentioning
confidence: 99%