Multi-species biofilm of Candida albicans and non-Candida albicans Candida species on acrylic substrate

Abstract

Objective In polymicrobial biofilms bacteria extensively interact with Candida species, but the interaction among the different species of the Candida is yet to be completely evaluated. In the present study, the difference in biofilm formation ability of clinical isolates of four species of Candida in both single-species and multi-species combinations on the surface of dental acrylic resin strips was evaluated. Material and Methods The species of Candida , isolated from multiple species oral candidiasis of the neutropenic patients, were used for the experiment. Organisms were cultured on Sabouraud dextrose broth with 8% glucose (SDB). Biofilm production on the acrylic resins strips was determined by crystal violet assay. Student's t-test and ANOVA were used to compare in vitro biofilm formation for the individual species of Candida and its different multi-species combinations. Results In the present study, differences between the mean values of the biofilm-forming ability of individual species ( C. glabrata>C. krusei>C. tropicalis>C. albicans ) and in its multi-species' combinations (the highest for C. albicans with C. glabrata and the lowest for all the four species combination) were reported. Conclusions The findings of this study showed that biofilm-forming ability was found greater for non- Candida albicans Candida species (NCAC) than for C. albicans species with intra-species variation. Presence of C. albicans in multi-species biofilms increased, whereas; C. tropicalis decreased the biofilm production with all other NCAC species.

Study of Microbial Interaction Formed by "Candida krusei" and "Candida glabrata": "In Vitro" and "In Vivo" Studies

“…Single biofilms produced by C. glabrata showed the highest metabolic activity than that produced by C. krusei, although it produced less biomass. These results were similar with other reports (12,(22)(23)(24). Consequently, C. glabrata cells in the biofilms seem to be metabolically more active than cells from other species that produce biofilms with more biomass.…”

“…In mixed biofilms, the metabolic activity was reduced significantly in comparison with single biofilm of C. glabrata, and it was significantly similar to the single biofilm of C. krusei, suggesting a possible competition between the species. These findings corroborate with Pathak et al (12) that evaluated the interaction of these species by XTT assay and found C. krusei decreased the biofilm production of C. glabrata.…”

“…There is just one study that investigated the in vitro interaction between C. krusei and C. glabrata (12) and our study is the first one that has additional in vitro data and also in vivo. In this context, the objective of this study was to evaluate the interactions between C. krusei and C. glabrata in in vitro biofilms and its ability to colonize the oral cavity of mice.…”

Mixed biofilms formed by C. albicans and non-albicans species: a study of microbial interactions

“…However, the presence of C. krusei in the mixed biofilm reduced cell viability, compared with the cell viability observed in the single biofilm formed by C. albicans. Pathak et al 28 also studied the interactions among C. albicans, C. glabrata, C. tropicalis and C. krusei in vitro through optical density values using the crystal violet assay. C. tropicalis decreased the biofilm production of non-albicans species.…”

Novas tendências de combate ao biofilme de Candida em próteses dentárias

“…MQS são moléculas autoindutoras que à medida que crescem proporcionam um aumento da população. Após alcançar um limiar crítico, é desenvolvida uma resposta regulatória de expressão/inibição que é monitorada por genes 44 Pathak et al 46 realizaram um estudo utilizando a espécie de Candida (C. albicans, C. glabrata, C. krusei e C. tropicalis) e multiespécies da microbiota oral de pacientes neutropênicos. A produção do biofilme na resina acrílica foi avaliada pelo método cristal violeta.…”