Multifunctional Roles of Plant Dehydrins in Response to Environmental Stresses

Liu, Yang; Song, Qiping; Li, Daxing; Yang, Xinghong; Li, Dequan

doi:10.3389/fpls.2017.01018

Cited by 106 publications

(85 citation statements)

References 42 publications

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“…Over the past few decades, numerous DHN genes have been identified in various plant species, and were demonstrated to play important roles in abiotic stress tolerance (Banerjee and Roychoudhury 2016 ; Liu et al 2017 ). In this study, a DHN gene named CsLEA11 was for the first time isolated and characterized from C. sativus .…”

Section: Discussionmentioning

confidence: 99%

Overexpression of CsLEA11, a Y3SK2-type dehydrin gene from cucumber (Cucumis sativus), enhances tolerance to heat and cold in Escherichia coli

Zhou

et al. 2017

AMB Expr

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As the group II LEA (late embryogenesis abundant) proteins, dehydrins (DHNs) play an important role in plant growth and development, as well as in response to abiotic or biotic stress challenges. In this study, a DHN gene named CsLEA11 was identified and characterized from Cucumis sativus. Sequence analysis of CsLEA11 showed that it is a Y3SK2-type DHN protein rich in hydrophilic amino acids. Expression analyses revealed that the transcription of CsLEA11 could be significantly induced by heat and cold stress. The recombinant plasmid was transformed into Escherichia coli BL21 and isopropy-β-d-thiogalactoside (IPTG) was used to induce recombinant E. coli to express CsLEA11 gene. Overexpression of CsLEA11 in E. coli enhanced cell viability and conferred tolerance to heat and cold stress. Furthermore, CsLEA11 protein could protect the activity of lactate dehydrogenase (LDH) under heat stress. Taken together, our data demonstrate that CsLEA11 might function in tolerance of cucumber to heat and cold stress.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-017-0483-1) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Overexpression of CsLEA11, a Y3SK2-type dehydrin gene from cucumber (Cucumis sativus), enhances tolerance to heat and cold in Escherichia coli

Zhou

et al. 2017

AMB Expr

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“…Dehydrin (DHN) is classified as a LEA family due to the gene expression during late seed embryogenesis and ability to perform 'classical' chaperone activity, preventing heat-induced protein aggregation and inactivation in vitro (Kovacs et al, 2008;Liu et al, 2017). In our dataset, we found that DHN genes are distributed in two main angiospermwide synteny communities and a maximum likelihood tree supports the phylogenetic separation of these communities in angiosperms (Figure 3a).…”

Section: Dehydrin: Functional Diversification Via Biochemical Structsupporting

confidence: 56%

Synteny-based phylogenomic networks for comparative genomics

Zhao

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“…Dehydrin (DHN) is classified as a LEA family due to the gene expression during late seed embryogenesis and ability to perform 'classical' chaperone activity, preventing heat-induced protein aggregation and inactivation in vitro (Kovacs et al, 2008;Liu et al, 2017a). In our dataset, we found that DHN genes are distributed across two main angiosperm-wide synteny communities and a maximum likelihood tree supports the phylogenetic separation of these communities in angiosperms ( Figure 3a).…”

Section: Dehydrin: Biochemical Structural and Expression Pattern Insupporting

confidence: 53%

Unfolding plant desiccation tolerance : evolution, structure, and function of LEA proteins

Artur¹,

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Living beings comprise a whole sequence of levels forming [such] a hierarchy. (…) Each level relies for its operations on all the levels below it. Each reduces the scope of the one immediately below it by imposing on it a boundary that harnesses it to the service of the next-higher level, and this control is transmitted stage by stage, down to the basic inanimate level. (...) Each separate level of existence is of course interesting in itself and can be studied in itself." (Polanyi, 1968) "The ability to reduce everything to simple fundamental laws does not imply the ability to start from those laws and reconstruct the universe. (…) At each stage entirely new laws, concepts, and generalizations are necessary, requiring inspiration and creativity to just as great a degree as in the previous one. We expect to encounter fascinating and, I believe, very fundamental questions at each stage in fitting together less complicated pieces into the more complicated system and understanding the basically new types of behaviour which can result." (Anderson, 1972) In the research field of desiccation tolerance (DT), we aim to 'unfold' the different underpinnings of life without water in tolerant plant species in order to 'unlock' this process in non-tolerant species. The idea of 'unlocking' DT arises in a context where food productivity and security are increasingly challenged by environmental changes such as increased drought, while the world population is predicted to steadily grow to over 8.3 billion of people in the year 2030 (FAO, 2017). In the development of new technologies to tackle these challenges, raising 'basic' questions and conducting fundamental research are of pivotal importance. In this thesis I will present results of a study encompassing genomics, evolution, protein biochemistry, and molecular biology, and then go to a more general and speculative discussion of the main findings. Such a multilevel approach can aid to the comprehension of the complexity of the different levels that the process of DT involves, and can provide transferrable knowledge to produce more stress-tolerant crops.

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Multifunctional Roles of Plant Dehydrins in Response to Environmental Stresses

Cited by 106 publications

References 42 publications

Overexpression of CsLEA11, a Y3SK2-type dehydrin gene from cucumber (Cucumis sativus), enhances tolerance to heat and cold in Escherichia coli

Overexpression of CsLEA11, a Y3SK2-type dehydrin gene from cucumber (Cucumis sativus), enhances tolerance to heat and cold in Escherichia coli

Synteny-based phylogenomic networks for comparative genomics

Unfolding plant desiccation tolerance : evolution, structure, and function of LEA proteins

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