Multiplex detection of foodborne pathogens by real-time loop-mediated isothermal amplification on a digital microfluidic chip

Xie, Mei; Chen, TianLan; Xiong, Xin; Cai, Zongwei; Dong, Cheng; Lei, Bo

doi:10.1016/j.foodcont.2022.108824

Cited by 45 publications

(22 citation statements)

References 37 publications

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“…All operations on the DMF chip were managed using an integrated control system (Digifluidic, Guangdong, China) as described in our previous study. 28 In addition, the images of the droplets were captured by a smartphone (iPhone 13) for RGB-based image processing analysis.…”

Section: Chip Design Fabrication and Operationmentioning

confidence: 99%

“…The application of DMF-based NAT for the detection of various pathogens has been evidenced in previous studies. [27][28][29][30] Among various nucleic acid amplification techniques, loop-mediated isothermal amplification (LAMP) has received wide attention in POCT due to its advantages of rapid reaction, good specificity, a simple requirement for experimental settings, and versatile detection modalities. 31 Common strategies for the confirmation of LAMP amplicons mainly include fluorescence detection, turbidity measurement and colorimetric detection, and current POCTbased detection primarily relies on optical technologies.…”

Section: Introductionmentioning

confidence: 99%

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A digital microfluidic platform coupled with colorimetric loop-mediated isothermal amplification for on-site visual diagnosis of multiple diseases

Xie

Chen²,

Cai

et al. 2023

Lab Chip

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Section: Chip Design Fabrication and Operationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A digital microfluidic platform coupled with colorimetric loop-mediated isothermal amplification for on-site visual diagnosis of multiple diseases

Xie

Chen²,

Cai

et al. 2023

Lab Chip

Self Cite

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“…LAMP is a mature isothermal nucleic acid amplification technique in which the target sequence was amplified with two or three sets of primers at a constant temperature of 60–65°C ( Prasannakumar et al, 2020 ; Wang et al, 2020f ; Zhang et al, 2021b ). Typically, four different primers are used to amplify six different regions on the target gene, which increases specificity ( Xie et al, 2022 ). Another pair of “cyclic primers” can further accelerate the reaction.…”

Section: Molecular Biology Detection Technologymentioning

confidence: 99%

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Zhao

Huang

et al. 2022

Front. Bioeng. Biotechnol.

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The global burden of foodborne disease is enormous and foodborne pathogens are the leading cause of human illnesses. The detection of foodborne pathogenic bacteria has become a research hotspot in recent years. Rapid detection methods based on immunoassay, molecular biology, microfluidic chip, metabolism, biosensor, and mass spectrometry have developed rapidly and become the main methods for the detection of foodborne pathogens. This study reviewed a variety of rapid detection methods in recent years. The research advances are introduced based on the above technical methods for the rapid detection of foodborne pathogenic bacteria. The study also discusses the limitations of existing methods and their advantages and future development direction, to form an overall understanding of the detection methods, and for point-of-care testing (POCT) applications to accurately and rapidly diagnose and control diseases.

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“…Recently, several efforts have been made to develop innovative integrated biosensing methodologies, including microfluidic platforms, lateral flow tests, and smartphone-assisted sensors [ 120 , 121 ]. Digital microfluidics with loop-mediated isothermal amplification (LAMP) have been reported for the multiplex detection of different pathogens (refer to Figure 6 ) [ 122 ]. The use of microfluidics technology requires a minimal sample amount to localize target analyte under flow conditions in a sensing area of a chip with small dimensions.…”

Section: Emerging Pathogen-detection Techniquesmentioning

confidence: 99%

Current Scenario of Pathogen Detection Techniques in Agro-Food Sector

Nehra

Kumar

et al. 2022

Biosensors

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Over the past-decade, agricultural products (such as vegetables and fruits) have been reported as the major vehicles for foodborne diseases, which are limiting food resources. The spread of infectious diseases due to foodborne pathogens poses a global threat to human health and the economy. The accurate and timely detection of infectious disease and of causative pathogens is crucial in the prevention and treatment of disease. Negligence in the detection of pathogenic substances can be catastrophic and lead to a pandemic. Despite the revolution in health diagnostics, much attention has been paid to the agro-food sector regarding the detection of food contaminants (such as pathogens). The conventional analytical techniques for pathogen detection are reliable and still in operation. However, laborious procedures and time-consuming detection via these approaches emphasize the need for simple, easy-to-use, and affordable detection techniques. The rapid detection of pathogens from food is essential to avoid the morbidity and mortality originating from the suboptimal nature of empiric pathogen treatment. This review critically discusses both the conventional and emerging bio-molecular approaches for pathogen detection in agro-food.

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Multiplex detection of foodborne pathogens by real-time loop-mediated isothermal amplification on a digital microfluidic chip

Cited by 45 publications

References 37 publications

A digital microfluidic platform coupled with colorimetric loop-mediated isothermal amplification for on-site visual diagnosis of multiple diseases

A digital microfluidic platform coupled with colorimetric loop-mediated isothermal amplification for on-site visual diagnosis of multiple diseases

Research progress on detection techniques for point-of-care testing of foodborne pathogens

Current Scenario of Pathogen Detection Techniques in Agro-Food Sector

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