Mutagenic potential of medicinal plants evaluated by the Ames Salmonella/microsome assay: A systematic review

Dantas, Fabiana Gomes da Silva; Castilho, Pamella Fukuda de; Almeida-Apolonio, Adriana Araújo de; Araújo, Renata Pires de; Oliveira, Kelly Mari Pires de

doi:10.1016/j.mrrev.2020.108338

Cited by 21 publications

(14 citation statements)

References 134 publications

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“…In recent years, the number of studies using this type of assay to evaluate plant extracts’ genotoxic/mutagenic potential has increased [ 40 ]. Studies have reported that several plants used in traditional medicine are potentially genotoxic [ 40 , 41 ] and some of the species identified as genotoxic belong to the Anacardiaceae family (e.g., Anacardium ocidentale , Taipirira guianensis ) [ 42 ], including one species from the genus Lannea [ 43 ]. According to these authors, Lannea edulis presented marginal genotoxicity in the strain TA97, but Lannea discolor did not [ 43 ].…”

Section: Resultsmentioning

confidence: 99%

Contribution to the Preclinical Safety Assessment of Lannea velutina and Sorindeia juglandifolia Leaves

Malú

Lima

Malmir

et al. 2022

Plants

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Dried leaves of Lannea velutina A. Rich. and Sorindeia juglandifolia (A. Rich.) Planch. ex Oliv. (family Anacardiaceae) are used in African traditional medicine. Although these medicinal plants have widespread use in the treatment of inflammatory diseases, there is no scientific data concerning their preclinical or clinical safety. This work aimed to investigate the phytochemical properties of the leaves of both species using HPLC-UV/DAD, as well as the in vivo oral repeated-dose toxicity of 70% hydroethanolic leaf extract of S. juglandifolia and the in vitro genotoxicity of 70% hydroethanolic leaf extracts of L. velutina and S. juglandifolia. Clinical signs of toxicity, body weight variations, and changes in food consumption, mortality, and blood biochemical parameters were monitored. Genotoxicity was assessed using the bacterial reverse mutation assay (Ames test) with and without metabolic activation, according to OECD guidelines. The obtained results showed the presence of gallic acid and anacardic acid as the main marker constituents in both species. No significant changes in general body weight or food intake were observed; small significant changes with no critical relevance were observed in the blood biochemistry of animals treated with S. juglandifolia hydroethanolic extract (50, 400, and 1000 mg/kg body weight) compared to those in the control group. No genotoxicity was observed in the bacterial reverse mutation assay with S. juglandifolia and L. velutina extracts (up to 5 mg/plate). The safety data obtained in vivo and lack of genotoxic potential in vitro points to the safe medicinal use of S. juglandifolia and L. velutina extracts.

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Section: Resultsmentioning

confidence: 99%

Contribution to the Preclinical Safety Assessment of Lannea velutina and Sorindeia juglandifolia Leaves

Malú

Lima

Malmir

et al. 2022

Plants

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“…Based on the results presented in Table 3, in which selected strains TA98, TA100, and TA102 show a 93% prediction of detecting gene mutations, 91,92 we can conclude that hit 15g does not have mutagenic potential, despite exhibited cytotoxic potential only at the higher concentration tested (5000 μg) against TA98 and TA100 (MI ≤ 0.7), and thus, this is a strong candidate for further exploration at in vivo tests. Moreover, these findings are contrary to previous in silico prediction of Ames test toxicity for 15g (Supporting Information, Table S2), it is crucial to conduct bench tests to validate the results obtained through computer simulations, highlighting their significance.…”

Section: ■ Chemistry Remarksmentioning

confidence: 88%

Design and Synthesis of Novel 3-Nitro-1H-1,2,4-triazole-1,2,3-triazole-1,4-disubstituted Analogs as Promising Antitrypanosomatid Agents: Evaluation of In Vitro Activity against Chagas Disease and Leishmaniasis

Pelizaro,

Batista,

Portapilla

et al. 2024

J. Med. Chem.

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A series of 28 compounds, 3-nitro-1H-1,2,4-triazole, were synthesized by click-chemistry with diverse substitution patterns using medicinal chemistry approaches, such as bioisosterism, Craig-plot, and the Topliss set with excellent yields. Overall, the analogs demonstrated relevant in vitro antitrypanosomatid activity. Analog 15g (R 1 = 4-OCF 3 −Ph, IC 50 = 0.09 μM, SI = >555.5) exhibited an outstanding antichagasic activity (Trypanosoma cruzi, Tulahuen LacZ strain) 68-fold more active than benznidazole (BZN, IC 50 = 6.15 μM, SI = >8.13) with relevant selectivity index, and suitable LipE = 5.31. 15g was considered an appropriate substrate for the type I nitro reductases (TcNTR I), contributing to a likely potential mechanism of action for antichagasic activity. Finally, 15g showed nonmutagenic potential against Salmonella typhimurium strains (TA98, TA100, and TA102). Therefore, 3-nitro-1H-1,2,4-triazole 15g is a promising antitrypanosomatid candidate for in vivo studies.

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“…The Ames test measures the mutagenic potential of chemicals [10,11]. Most of the mutagenity tests were performed on bacteria.…”

Section: Introductionmentioning

confidence: 99%

“…Most of the mutagenity tests were performed on bacteria. The Ames microplate fluctuation (MPF) test has essentially the same principles as the traditional Ames test, but new standards have been established for this type of test [10,11,12]. It has many advantages over the traditional Ames test and complies with OECD (Organization for Economic Co-operation and Development) and FDA (Food and Drug Administration) guidelines.…”

Section: Introductionmentioning

confidence: 99%

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Chemical composition and in vitro mutagenic, antioxidant and anti-inflammatory activities of Lavandula angustifolia Mill. essential oil from Turkey

ÖZGEN¹,

BİLTEKİN²,

İDUĞ³

et al. 2022

jrp

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The essential oil obtained from Lavandula angustifolia Mill. (Lavander) has many pharmacological effects, including antioxidant, antifungal, antibacterial and carminative effects. Lavandula angustifolia essential oil (LEO) contains monoterpene compounds, mainly linalool and linalyl acetate and various phenolic acids such as caffeic acid, vanillic acid, ferulic acid and chlorogenic acid. The hydrodistillation of the aerial parts of L. angustifolia produced a pale yellow oil. Gas chromatography-Gas chromatography/Massspectrometry (GC-GC/MS) results showed that LEO contains monoterpene alcohols that primarily include linalool (35.91%), 4-Terpineol (6.10%), α-Terpineol (4.49%) and lavandulol (2.49%). As a result of the cytotoxicity analysis of LEO, the IC50 value was found to be 0.372 mg/mL. LEO was found to be non-mutagenic against the bacterial test strain Salmonella typhimurium TA 98 with S9 fraction while the highest concentration (9.58 mg/mL) was found to be either mutagenic or cytotoxic against TA 98 without S9 fraction. The essential oil's antioxidant capacity was found 6.522±0.069 mg/mL while the total phenolic content was 1.22±0.04 mg GAE/g extract (Gallic acid equivalent/g extract). Anti-inflammatory activity of LEO was 1.238± 0.026 mg/mL while that of indomethacine was 0.022±0.003 mg/mL. In conclusion, when the results are evaluated in terms of in vitro biological activities, LEO can be considered as a potential herbal product with active compounds that should be examined in future studies.

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Mutagenic potential of medicinal plants evaluated by the Ames Salmonella/microsome assay: A systematic review

Cited by 21 publications

References 134 publications

Contribution to the Preclinical Safety Assessment of Lannea velutina and Sorindeia juglandifolia Leaves

Contribution to the Preclinical Safety Assessment of Lannea velutina and Sorindeia juglandifolia Leaves

Design and Synthesis of Novel 3-Nitro-1H-1,2,4-triazole-1,2,3-triazole-1,4-disubstituted Analogs as Promising Antitrypanosomatid Agents: Evaluation of In Vitro Activity against Chagas Disease and Leishmaniasis

Chemical composition and in vitro mutagenic, antioxidant and anti-inflammatory activities of Lavandula angustifolia Mill. essential oil from Turkey

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