Mutations at codon position 1999 of acetyl‐CoA carboxylase confer resistance to ACCase‐inhibiting herbicides in Japanese foxtail (Alopecurus japonicus)

Abstract

This study revealed that A. japonicus had multiple copies of genes encoding plastidic ACCase, and each gene was able to carry its own mutation. It also established the clear importance of the W1999C and W1999L mutations in conferring resistance to ACCase-inhibiting herbicides.

Trp‐1999‐Ser mutation of acetyl‐CoA carboxylase and cytochrome P450s‐involved metabolism confer resistance to fenoxaprop‐P‐ethyl in Polypogon fugax

“…), Japanese foxtail ( Alopecurus japonicus Steud. ), keng stiffgrass ( Pseudosclerochloa kengiana (Ohwi) Tzvel. ), and hexaploid A. fatua .…”

“…We further found that all resistant plants ( n ≥ 50) carrying the Trp‐1999‐Ser mutation were heterozygous using the dCAPS genotyping. Similarly, when studying the mutation(s) of ACCase in the tetraploid P. kengiana and A. japonicus and hexaploid A. fatua , no homozygous‐resistant genotype was detected using dCAPS markers. This could be explained by the primers used for dCAPS amplifying the same region of multiple ACCase alleles.…”

“…Subsequently, Trp‐1999‐Leu/Ser mutations were successively detected in different grass species . In A. sterilis and A. japonicus , such mutations are able to confer resistance to fenoxaprop but not to clodinafop, haloxyfop, and sethoxydim . For the Trp‐1999‐Ser mutant plants especially, Italian ryegrass ( Lolium multiflorum Lam.…”

Molecular basis of ALS- and/or ACCase-inhibitor resistance in shortawn foxtail (Alopecurus aequalis Sobol.)

“…In China, sustained reliance on ACCase and/or ALS inhibitors to control weeds has led to the evolution of many resistant populations resulting from mutant ALS and ACCase [16,20]. In the JTJY-1 population, the presence of two different TSR mechanisms (ALS and ACCase mutations) in individual plants has appeared.…”

“…Three sets of primers (Table 3) spanning all eight known mutation sites affecting ALS efficacy based on Alopecurus myosuroides (AJ437300) sequence available in GenBank, were designed using the Primer Premier 5.0 software, and two primers (Table 3) previously used in a study by Xu et al [16] were used to amplify and sequence ACCase gene fragments of A. aequalis.…”