Nano-graphene oxide with antisense <i>vicR</i> RNA reduced exopolysaccharide synthesis and biofilm aggregation for <i>Streptococcus mutans </i>

Abstract

Streptococcus mutans (S. mutans) has been proved to crucial cariogenic pathogens. Antisense vicR RNA reduced the transcription of virulence genes and lead to a reduction in biofilm formation. In the current study, a graphene-oxide plasmid transformation system was developed using interacted GO-polyethylenimine (PEI) complexes loaded with antisense vicR-expressing plasmid (GO-PEI-ASvicR). The particle size distribution and zeta potential of the GO-PEI-based ASvicR were evaluated. Quantitative real-time PCR assays were used to investigate the expression of S. mutans virulence genes. The exopolysaccharide (EPS) production in biofilm were evaluated by confocal laser scanning microscopy and anthrone method. We showed that GO-PEI could efficiently deliver the ASvicR-expressing plasmid into S. mutans cells and support excellent transcripts of ASvicR. Furthermore, GO-PEI-ASvicR significantly reduced virulent-associated gene expressions, suppressed biofilm aggregation and inhibited EPS accumulation. Our reports demonstrated that preserving nano-graphene oxide with antisense vicR RNA will be a more effective strategy for dental caries management.

Propolis nanoparticle enhances the potency of antimicrobial photodynamic therapy against Streptococcus mutans in a synergistic manner

“…Also, the results of the present study showed that gtfC was more affected in the combination-treatment group than the other genes tested. Deletion of gtfC markedly reduces attachment to smooth surfaces by S. mutans 35 .…”

Antimicrobial and Antibiofilm Coating of Dental Implants—Past and New Perspectives

“…Some examples of biofilm-related genes are motA gene, encoding the element of the flagellar motor complex, the efaA gene, which plays an important role in the adhesion of bacteria to surfaces [ 46 , 47 ]. ASOs can also block two-component signal transduction systems, such as VicRK, that induce the gene expression for the synthesis of extracellular insoluble glucan, an extracellular matrix component of biofilms [ 48 ].…”

“…In 2020 Wu S. et al developed a graphene oxide (GO)-based plasmid transformation system using electrostatic interacted GO-polyethyleneimine (PEI) complexes loaded with antisense vicR plasmid (GO-PEI-ASvicR). They showed that GO-PEI could efficiently deliver ASvicR plasmids into S. mutans cells with excellent transcripts of ASvicR, significantly reducing biofilm aggregation and exopolysaccharide (EPS) accumulation [ 48 ]. It is worth noting that graphene-based coating on titanium surfaces can be successfully obtained by electrodeposition as shown by Jankovic A (2015), making it a feasible option for innovative titanium coatings.…”

The Action of Efflux Pump Genes in Conferring Drug Resistance to Klebsiella Species and Their Inhibition

“…Several studies have demonstrated the therapeutic efficacy and applications of the ASOs as mechanisms of inhibition to design therapies for bacterial infections. [74][75][76][77][78][79][80][81][82][83] A similar strategy of ASOs could be exploited against the MDR efflux system of K. pneumoniae. Versatility and high specificity of complementarity of the antisense oligonucleotides allow the generation of antisense drugs that disable resistance to antimicrobials and act as adjuvants to join the armamentarium to fight multidrug resistance.…”