Nanofibrous Lipid Membranes Capable of Functionally Immobilizing Antibodies and Capturing Specific Cells

Zha, Zhengbao; Cohn, Celine; Dai, Zhifei; Qiu, Weiguo; Jin-hong, Zhang; Wu, Xiaoyi

doi:10.1002/adma.201101516

Cited by 27 publications

(34 citation statements)

References 34 publications

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“…However, the selection of protein-or peptide-based functionalities that can be incorporated onto synthetic polymer nanofibers may be limited by the harsh conditions of fiber spinning or by the particular conjugation chemistry that only works for certain biomolecules. Here, we envision that the coating of electrospun polycaprolactone (PCL) fibers with cholesteryl succinyl silane (CSS) and the subsequent CSS-mediated anti- [19]. Recently, we have demonstrated that the CSS fibers possess a greater ability to functionally immobilize antibodies for cell capture than the PCL and plasmatreated PCL fibers do [20].…”

Section: Introductionmentioning

confidence: 99%

Lipid-mediated protein functionalization of electrospun polycaprolactone fibers

Cohn¹,

Leung²,

Crosby³

et al. 2016

Express Polym. Lett.

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Abstract. In this study, electrospun polycaprolactone (PCL) fibers are plasma-treated and chemically conjugated with cholesteryl succinyl silane (CSS). In addition to Raman spectroscopy, an immobilization study of DiO as a fluorescent probe of lipid membranes provides evidence supporting the CSS coating of plasma-treated PCL fibers. Further, anti-CD20 antibodies are used as a model protein to evaluate the potential of lipid-mediated protein immobilization as a mechanism to functionalize the CSS-PCL fiber scaffolds. Upon anti-CD20 functionalization, the CSS-PCL fiber scaffolds capture Granta-22 cells 2.4 times more than the PCL control does, although the two fiber scaffolds immobilize a comparable amount of anti-CD20. Taken together, results from the present study demonstrate that the CSS coating and CSS-mediated antibody immobilization offers an appealing strategy to functionalize electrospun synthetic polymer fibers and confer cell-specific functions on the fiber scaffolds, which can be mechanically robust but often lack biological functions.

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Section: Introductionmentioning

confidence: 99%

Lipid-mediated protein functionalization of electrospun polycaprolactone fibers

Cohn¹,

Leung²,

Crosby³

et al. 2016

Express Polym. Lett.

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“…The CSS nanofibers were formed via a combined sol-gel and electrospinning mechanism. 5 As illustrated in Fig. 1(b), the CSS nanofibers are composed of multiple layers of entangled cylindrical micelles.…”

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confidence: 99%

“…Previously, we reported that electrospun organic-inorganic lipid nanofibers are capable of functionally immobilizing Abs and capturing targeted cells without any chemical treatment. 5 In this study, we examine the underlying mechanism of Ab immobilization on the electrospun lipid nanofibers and illustrate the nanotopographic effects of the lipid nanofibers on cell capture.…”

mentioning

confidence: 99%

“…Recently, the potential of functionalized electrospun nanofibers as probes for cell capture has been investigated. 5,6 Often ligands such as antibodies (Abs) 2,4,6 and aptamers 7 that specifically target the cellular receptors of CTCs are chemically conjugated to the micro-/nanostructured probes in a multi-step procedure. Previously, we reported that electrospun organic-inorganic lipid nanofibers are capable of functionally immobilizing Abs and capturing targeted cells without any chemical treatment.…”

mentioning

confidence: 99%

“…1(a)], as reported in our previous studies. 5,8 The CSS powders were dissolved in a mixture of 1 ml tetrahydrofuran (THF) and 10 ll 37% hydrochloric acid (HCl). Due to the existence of a small amount of water in the mixed solvent, the CSS molecules were hydrolyzed and partially polymerized after 8 h of incubation.…”

mentioning

confidence: 99%

See 2 more Smart Citations

A biomimetic mechanism for antibody immobilization on lipid nanofibers for cell capture

Zha¹,

Jiang²,

Dai

et al. 2012

Applied Physics Letters

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The immobilization of membrane-bound molecules on organic-inorganic cholesteryl-succinyl silane (CSS) nanofibers is investigated. Fluorescent microscopy and a cell capture assay confirm the stable and functional immobilization of membrane-bound antibodies and imaging agents on the electrospun CSS nanofibers. An insert-and-tighten mechanism is proposed for the observed hydration-induced reduction in lipid nanofiber diameter, the immobilization of membrane-bound molecules, and the improved efficiency of cell capture by the functionalized CSS nanofibers over their film counterparts. The ability to stably and functionally immobilize membrane-bound molecules on the CSS nanofibers presents a promising method to functionalize lipid-based nanomaterials.

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Tumor Therapy Strategies Based on Microenvironment‐Specific Responsive Nanomaterials

Zhang

Ding

Sun

et al. 2023

Adv Healthcare Materials

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The tumor microenvironment (TME) is a complex and variable region characterized by hypoxia, low pH, high redox status, overexpression of enzymes, and high‐adenosine triphosphate concentrations. In recent years, with the continuous in‐depth study of nanomaterials, more and more TME‐specific response nanomaterials are used for tumor treatment. However, the complexity of the TME causes different types of responses with various strategies and mechanisms of action. Aiming to systematically demonstrate the recent advances in research on TME‐responsive nanomaterials, this work summarizes the characteristics of TME and outlines the strategies of different TME responses. Representative reaction types are illustrated and their merits and demerits are analyzed. Finally, forward‐looking views on TME‐response strategies for nanomaterials are presented. It is envisaged that such emerging strategies for the treatment of cancer are expected to exhibit dramatic trans‐clinical capabilities, demonstrating the extensive potential for the diagnosis and therapy of cancer.

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Nanofibrous Lipid Membranes Capable of Functionally Immobilizing Antibodies and Capturing Specific Cells

Cited by 27 publications

References 34 publications

Lipid-mediated protein functionalization of electrospun polycaprolactone fibers

Lipid-mediated protein functionalization of electrospun polycaprolactone fibers

A biomimetic mechanism for antibody immobilization on lipid nanofibers for cell capture

Tumor Therapy Strategies Based on Microenvironment‐Specific Responsive Nanomaterials

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