Nanoscale fluoro-immunoassays with lanthanide oxide nanoparticles

Kennedy, Ian M.; Koivunen, Marja M.; Gee, S.; Cummins, C.M.; Perron, Richard; Dosev, Dosi; Hammock, Bruce D.

doi:10.1117/12.571483

Cited by 4 publications

(2 citation statements)

References 33 publications

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“…The lanthanide oxides are also useful as nanoparticle labels in biology, providing some benefits over the more widely known quantum dot materials of CdSe and ZnS. As an example, it has been shown that it is possible to carry out highly sensitive immunoassays using Eu and Tb oxide nanoparticles (Feng et al 2003; Kennedy et al 2004) for monitoring toxins in the environment.…”

Section: Introductionmentioning

confidence: 99%

Effect of cerium oxide nanoparticles on inflammation in vascular endothelial cells

Gojová

Lee

Jung

et al. 2009

Inhalation Toxicology

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Because vascular endothelial cell inflammation is critical in the development of cardiovascular pathology, we hypothesized that direct exposure of human aortic endothelial cells (HAECs) to ultrafine particles induces an inflammatory response. To test the hypothesis, we incubated HAECs for 4 h with different concentrations (0.001-50 μg/ml) of CeO 2 nanoparticles and subsequently measured mRNA levels of the three inflammatory markers intercellular adhesion molecule 1 (ICAM-1), interleukin (IL)-8, and monocyte chemotactic protein (MCP-1) using real-time polymerase chain reaction (PCR). Ceria nanoparticles caused very little inflammatory response in HAECs, even at the highest dose. This material is apparently rather benign in comparison with Y 2 O 3 and ZnO nanoparticles that we have studied previously. These results suggest that inflammation in HAECs following acute exposure to metal oxide nanoparticles depends strongly on particle composition.

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Section: Introductionmentioning

confidence: 99%

Effect of cerium oxide nanoparticles on inflammation in vascular endothelial cells

Gojová

Lee

Jung

et al. 2009

Inhalation Toxicology

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“…The enzyme-linked immuno sorbent assay (ELISA) is the technique most commonly used by molecular biologists for biomarker detection and quantification. ELISA tests are based on the principle of a solid phase enzyme-linked immunosorbent assay using the spectroscopic detection of a colored reagent [12,13]. The aims of the work are to preparation of more stable-dispersed and size-controlled of pure AuNPs and AgNPs in easy, fast and simple method with highly ablation efficiency and studying the effects of presence AuNPs and AgNPs on the levels of T3 in sera of patients with CRF whose their thyroid had hyperthyroidism and improved and modified a competitive ELISA method.…”

Section: Introductionmentioning

confidence: 99%

Biochemical study for gold and silver nanoparticles on thyroid hormone levels in saliva of patients with chronic renal failure

Jassim

Al-Kazaz

2013

Eur. J. Chem.

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KEYWORDSNoble metal nanoparticles were synthesized directly by pulsed laser ablation (Nd:YAG, λ=1064 nm) of gold and silver plates immersed in pure water. Concentrations of the nanoparticles were determined by atomic absorption spectroscopy measurement. Atomic force microscope and transmission electron microscope analysis were used to characterize the size and size distributions of the metals nanoparticles. The objective of this work is studying the effects of presence gold and silver nanoparticles on the levels of triiodothyronine hormone (T3) in saliva of patients with chronic renal failure their thyroid had disorder (hypothyroidism). Also the study characterized the binding between the antiT3antibody with its antigen i.e., T3 in saliva patients in the presence affixed size concentration of nanoparticles to improve and modify a competitive ELISA method. It is found that both gold and silver nanoparticles demonstrated activation effect on the binding between anti-T3 antibody and antigen (T3) in the saliva of patients and these effects increased with increasing the concentrations and size for both nanoparticles (gold was more activation effect than silver). Optimization of experimental conditions for the binding in the saliva were: 40 μL and 20 μL of saliva (T3) in presence both silver and gold nanoparticle, respectively, pH = 7.4 of 0.2 M phosphate buffer was selected as the detection solution, the temperature of incubation was 22 °C while the incubation time were 30 min, 90 min for silver and gold nanoparticles, respectively.

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