Nanosized zeolites as a perspective material for conductometric biosensors creation

Abstract

In this work, the method of enzyme adsorption on different zeolites and mesoporous silica spheres (MSS) was investigated for the creation of conductometric biosensors. The conductometric transducers consisted of gold interdigitated electrodes were placed on the ceramic support. The transducers were modified with zeolites and MSS, and then the enzymes were adsorbed on the transducer surface. Different methods of zeolite attachment to the transducer surface were used; drop coating with heating to 200°C turned out to be the best one. Nanozeolites beta and L, zeolite L, MSS, and silicalite-1 (80 to 450 nm) were tested as the adsorbents for enzyme urease. The biosensors with all tested particles except zeolite L had good analytical characteristics. Silicalite-1 (450 nm) was also used for adsorption of glucose oxidase, acetylcholinesterase, and butyrylcholinesterase. The glucose and acetylcholine biosensors were successfully created, whereas butyrylcholinesterase was not adsorbed on silicalite-1. The enzyme adsorption on zeolites and MSS is simple, quick, well reproducible, does not require use of toxic compounds, and therefore can be recommended for the development of biosensors when these advantages are especially important.

A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite

“…For the first time, the efficiency of various types of zeolites as carriers for the enzyme immobilization has been shown when developing conductometric biosensors [23–27]. The procedure of enzyme adsorption on silicalite was tested for a number of enzymes—acetylcholinesterase [27], urease [23, 25], recombinant urease [26], and butyrylcholinesterase [27].…”

“…The procedure of enzyme adsorption on silicalite was tested for a number of enzymes—acetylcholinesterase [27], urease [23, 25], recombinant urease [26], and butyrylcholinesterase [27]. Additionally, the effectiveness of this technique has been shown for enzyme biosensors based on pH-sensitive field-effect transistors [28–32].…”

Investigating the Influence of Temperature on the Kaolinite-Base Synthesis of Zeolite and Urease Immobilization for the Potential Fabrication of Electrochemical Urea Biosensors

“…The sensitivity and overall performance of enzymatic biosensors has improved tremendously over the years as a result of the synthesis and incorporation of nanomaterials in their fabrication [ 2 , 3 , 4 , 5 ]. These materials have excited engineers due to their unique structural, electrical, chemical and mechanical properties [ 6 , 7 , 8 , 9 ].…”

A polyaniline–zeolite nanocomposite material based acetylcholinesterase biosensor for the sensitive detection of acetylcholine and organophosphates

“…However, the overuse of these pesticides results in pesticide residues in the environment. [21][22][23][24][25][26][27] Very recently, our research group has shown the selective detection of neurotransmitters dopamine and epinephrine using porous polyaniline (PANI) and polyaniline-zeolite composite modified electrodes with high activity and sensitivity compared to the conventional polyaniline modified electrode. 17 Our research is focused on the synthesis of nanostructured metal oxides, polyaniline, zeolites and their nanocomposites and finds their applications in electrocatalysis.…”