Nettle Tea Inhibits Growth of Acute Myeloid Leukemia Cells In Vitro by Promoting Apoptosis

Hodroj, Mohammad Hassan; Bast, Nour al Hoda Al; Taleb, Robin I.; Borjac, Jamilah; Rizk, Sandra

doi:10.3390/nu12092629

Cited by 23 publications

(19 citation statements)

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“…Increased Bax/Bcl-2 ratio up-regulates caspase-3 activity and subsequently triggers programmed cell death in cancer cells [ 42 ]. Numerous literature have documented an important regulatory role of phytochemicals in Bax/Bcl-2/caspase-3 signaling in breast carcinogenesis [ 43 , 44 , 45 ]. This experiment clearly shows a significant dose-dependent increase in the Bax/Bcl-2 ratio in rat mammary tumors induced by sumac treatment, which correlates with an increase in cleaved caspase-3 expression in the treated group and higher sumac dose.…”

Section: Discussionmentioning

confidence: 99%

Rhus coriaria L. (Sumac) Demonstrates Oncostatic Activity in the Therapeutic and Preventive Model of Breast Carcinoma

Kubatka

Kello

Kajo

et al. 2020

IJMS

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Comprehensive scientific data provide evidence that isolated phytochemicals or whole plant foods may beneficially modify carcinogenesis. The aim of this study was to evaluate the oncostatic activities of Rhus coriaria L. (sumac) using animal models (rat and mouse), and cell lines of breast carcinoma. R. coriaria (as a powder) was administered through the diet at two concentrations (low dose: 0.1% (w/w) and high dose: 1 % (w/w)) for the duration of the experiment in a syngeneic 4T1 mouse and chemically-induced rat mammary carcinoma models. After autopsy, histopathological and molecular analyses of tumor samples in rodents were performed. Moreover, in vitro analyses using MCF-7 and MDA-MB-231 cells were conducted. The dominant metabolites present in tested R. coriaria methanolic extract were glycosides of gallic acid (possible gallotannins). In the mouse model, R. coriaria at a higher dose (1%) significantly decreased tumor volume by 27% when compared to controls. In addition, treated tumors showed significant dose-dependent decrease in mitotic activity index by 36.5% and 51% in comparison with the control group. In the chemoprevention study using rats, R. coriaria at a higher dose significantly reduced the tumor incidence by 20% and in lower dose non-significantly reduced tumor frequency by 29% when compared to controls. Evaluations of the mechanism of oncostatic action using valid clinical markers demonstrated several positive alterations in rat tumor cells after the treatment with R. coriaria. In this regard, histopathological analysis of treated tumor specimens showed robust dose-dependent decrease in the ratio of high-/low-grade carcinomas by 66% and 73% compared to controls. In treated rat carcinomas, we found significant caspase-3, Bax, and Bax/Bcl-2 expression increases; on the other side, a significant down-regulation of Bcl-2, Ki67, CD24, ALDH1, and EpCam expressions and MDA levels. When compared to control specimens, evaluation of epigenetic alterations in rat tumor cells in vivo showed significant dose-dependent decrease in lysine methylation status of H3K4m3 and H3K9m3 and dose-dependent increase in lysine acetylation in H4K16ac levels (H4K20m3 was not changed) in treated groups. However, only in lower dose of sumac were significant decreases in the expression of oncogenic miR210 and increase of tumor-suppressive miR145 (miR21, miR22, and miR155 were not changed) observed. Finally, only in lower sumac dose, significant decreases in methylation status of three out of five gene promoters–ATM, PTEN, and TIMP3 (PITX2 and RASSF1 promoters were not changed). In vitro evaluations using methanolic extract of R. coriaria showed significant anticancer efficacy in MCF-7 and MDA-MB-231 cells (using Resazurin, cell cycle, annexin V/PI, caspase-3/7, Bcl-2, PARP, and mitochondrial membrane potential analyses). In conclusion, sumac demonstrated significant oncostatic activities in rodent models of breast carcinoma that were validated by mechanistic studies in vivo and in vitro.

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Section: Discussionmentioning

confidence: 99%

Rhus coriaria L. (Sumac) Demonstrates Oncostatic Activity in the Therapeutic and Preventive Model of Breast Carcinoma

Kubatka

Kello

Kajo

et al. 2020

IJMS

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“…7 In addition to its antimicrobial effects, PHBA has been reported to possess a dose-dependent cytotoxic effect on prostate (PC-3), breast (MCF-7), and leukemic cancer cells. 8,9 PHBA has also been found to have an antihyperglycemic effect 10 in addition to its protective effect against atherosclerosis, inflammation, allergy, myocardial damage, and thrombosis. 11 Furthermore, PHBA and its derivatives 3, 4, 5-trihydroxybenzoic acid, ellagic acid, gallic acid, and pyrogallic acid are important molecules in the medicinal chemists' search for effective and safe molecules with antialgal activity.…”

Section: Introductionmentioning

confidence: 99%

“…In addition to its antimicrobial effects, PHBA has been reported to possess a dose‐dependent cytotoxic effect on prostate (PC‐3), breast (MCF‐7), and leukemic cancer cells 8,9 . PHBA has also been found to have an antihyperglycemic effect 10 in addition to its protective effect against atherosclerosis, inflammation, allergy, myocardial damage, and thrombosis 11 .…”

Section: Introductionmentioning

confidence: 99%

Improvement of caffeic acid biotransformation into para‐hydroxybenzoic acid by Candida albicans CI‐24 via gamma irradiation and model‐based optimization

Elleboudy

Elkhatib

Yassein

et al. 2021

Biotech and App Biochem

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Para‐hydroxybenzoic acid (PHBA) has great potential in biological applications due to its putative antiviral activity against SARS‐CoV‐2 and its antimicrobial activity in the face of the radically increasing number of multidrug‐resistant pathogens. This is in addition to its antimutagenic, anti‐inflammatory, antioxidant, hypoglycemic, antiestrogenic, and antiplatelet aggregating activities. In this study, an approximate sixfold increase in the production of PHBA was achieved via biotransformation of caffeic acid by Candida albicans. The improvement was performed in two steps: first, through mutation by gamma irradiation (5 KGy dose), resulting in the recovery of a mutant (CI‐24), which produced approximately triple the amount of PHBA produced by the wild‐type isolate. Then, biotransformation by this mutant was further optimized via response surface methodology model‐based optimization. The maximum PHBA production (7.47 mg/mL) was obtained in a fermentation medium composed of 1% w/v yeast extract as a nitrogen source, with an initial pH of 6.6, incubated at 28 °C at an agitation rate of 250 rpm. To further enhance the performance and economics of the process, cells of the CI‐24 mutant were immobilized in calcium alginate beads and could retain an equivalent biotransformation capacity after three successive biotransformation cycles.

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“…Urtica is rich in essential amino acids and minerals, as well as vitamins C, B, and K. Because of the huge nutritional value, urtica has been listed as a natural source of food flavorings in Europe and supplemented in soups and herbal teas (Joshi et al, 2014). Mohammad et al found that urtica extracts inhibit the growth of acute myeloid leukemia cells in vitro by promoting apoptosis (Hodroj et al, 2020). Both in vitro and in vivo proliferation of breast cancer cells can be suppressed by urticaria extracts, and they also enhance the sensitivity of paclitaxel drugs to breast cancer cells (Mohammadi et al, 2017) (Mohammadi et al, 2016).…”

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confidence: 99%

The analysis of flavonoids and triterpenes extracted from Urtica by LC‐MS and the antimicrobial activity of the extracts

Quan¹,

Sun²,

Liu³

et al. 2021

J. Food Process. Preserv.

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Functional foods and drug industries have been rapidly developed.Natural products can be used as antioxidants, antimicrobial agents, and raw materials in pharmaceutical production (Dai & Mumper, 2010).Bioactive substances extracted from plants are widely used in medicine and food. Several methods have been developed for extracting, isolating, and purifying flavonoids and triterpenoids. However, more simple, efficient, and less expensive methods to produce large-scale high-quality products are urgently needed.Urtica fissa E. Pritz., Urticaceae is a perennial herb with 100-180cm of the whole plant height, transverse rhizome, tetra-prismatic, densely ciliate and puberulent, sparsely branched. It is a common shadeloving plant, featured by vigorous life, rapid growth, lax soil requirements, favoring of warm and wet characteristics. It usually grows in the hillside, roadside, or house half shade wet place (Weigend, 2006).Urtica is a relatively common wild plant. Its generic name comes from the Latin word urere, meaning "to rub or burn." Stems and leaves of urtica are covered with fine cilia, containing ingredients such as formic acid and histamine. There is an intense burning sensation when stunning (Bourgeois et al., 2016). Urtica is also widely distributed geographically. It grows in Eurasia, North America, and North Africa,

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Nettle Tea Inhibits Growth of Acute Myeloid Leukemia Cells In Vitro by Promoting Apoptosis

Cited by 23 publications

References 50 publications

Rhus coriaria L. (Sumac) Demonstrates Oncostatic Activity in the Therapeutic and Preventive Model of Breast Carcinoma

Rhus coriaria L. (Sumac) Demonstrates Oncostatic Activity in the Therapeutic and Preventive Model of Breast Carcinoma

Improvement of caffeic acid biotransformation into para‐hydroxybenzoic acid by Candida albicans CI‐24 via gamma irradiation and model‐based optimization

The analysis of flavonoids and triterpenes extracted from Urtica by LC‐MS and the antimicrobial activity of the extracts

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