Neuro tissue engineering of glial nerve guides and the impact of different cell types

Lietz, Martin; Dreesmann, Lars; Hoss, Martin; Oberhoffner, Sven; Schlosshauer, Bürkhard

doi:10.1016/j.biomaterials.2005.08.007

Cited by 139 publications

(94 citation statements)

References 52 publications

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“…In vivo, nerve conduits seeded with aligned Schwann cells have been used [18,19]. In all cases, Schwann cells are observed to elongate and align parallel to the long axes of the micropatterns or the nerve conduits.…”

Section: Discussionmentioning

confidence: 99%

“…In all cases, Schwann cells are observed to elongate and align parallel to the long axes of the micropatterns or the nerve conduits. The main advantage of these aligned Schwann cells is the enhancement in the rate and extent of neurite elongation from dorsal root ganglia cultured on these aligned cells [19,20]. Although these studies suggest the potential of contact guidance and Schwann cell alignment on enhancing nerve regeneration, the associated functional changes in Schwann cells have not been evaluated.…”

Section: Discussionmentioning

confidence: 99%

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The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation

et al. 2008

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Peripheral nerve regeneration can be enhanced by the stimulation of formation of bands of Büngner prior to implantation. Aligned electrospun poly(ε-caprolactone) (PCL) fibers were fabricated to test their potential to provide contact guidance to human Schwann cells. After 7 days of culture, cell cytoskeleton and nuclei were observed to align and elongate along the fiber axes, emulating the structure of bands of Büngner. Microarray analysis revealed a general down-regulation in expression of neurotrophin and neurotrophic receptors in aligned cells as compared to cells seeded on twodimensional PCL film. Real-time-PCR analyses confirmed the up-regulation of early myelination marker, MAG, and the down-regulation of NCAM-1, a marker of immature Schwann cells. Similar gene expression changes were also observed on cells cultured on randomly-oriented PCL electrospun fibers. However, up-regulation of the myelin-specific gene, P0, was observed only on aligned electrospun fibers, suggesting the propensity of aligned fibers in promoting Schwann cell maturation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation

et al. 2008

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“…40 Bigger groove dimensions seem to be ineffective for neurite alignment of astrocytes 27 but not of dorsal root ganglion cells. 41 In addition to the alignment, the influence of surface topography on neurite length was investigated. On platinum, surface topography did not show any impact on neurite length in PC-12 cells.…”

Section: Discussionmentioning

confidence: 99%

Directing neuronal cell growth on implant material surfaces by microstructuring

et al. 2012

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CitationDirecting neuronal cell growth on implant material surfaces by microstructuring. 2012, 100 (4) Abstract: For best hearing sensation, electrodes of auditory prosthesis must have an optimal electrical contact to the respective neuronal cells. To improve the electrode-nerve interface, microstructuring of implant surfaces could guide neuronal cells toward the electrode contact. To this end, femtosecond laser ablation was used to generate linear microgrooves on the two currently relevant cochlear implant materials, silicone elastomer and platinum. Silicone surfaces were structured by two different methods, either directly, by laser ablation or indirectly, by imprinting using laser-microstructured molds. The influence of surface structuring on neurite outgrowth was investigated utilizing a neuronal-like cell line and primary auditory neurons. The pheochromocytoma cell line PC-12 and primary spiral ganglion cells were cultured on microstructured auditory implant materials. The orientation of neurite outgrowth relative to the microgrooves was determined. Both cell types showed a preferred orientation in parallel to the microstructures on both, platinum and on molded silicone elastomer. Interestingly, microstructures generated by direct laser ablation of silicone did not influence the orientation of either cell type. This shows that differences in the manufacturing procedures can affect the ability of microstructured implant surfaces to guide the growth of neurites. This is of particular importance for clinical applications, since the molding technique represents a reproducible, economic, and commercially feasible manufacturing procedure for the microstructured silicone surfaces of medical implants. V C 2012 Wiley Periodicals, Inc.J Biomed Mater Res Part B: Appl Biomater 00B: 000-000, 2012.

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“…The outer layer of fibroblasts between the regenerate and the conduit wall (Fig. 4a) may not inhibit axon regeneration (Lietz et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Assessment of Axonal Growth into Collagen Nerve Guides Containing VEGF‐Transfected Stem Cells in Matrigel

Kempton

Gonzalez

Leven

et al. 2008

The Anatomical Record

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The early events associated with axonal growth into 10-mm nerve gaps were studied histologically in the rat sciatic nerve model to determine if the outgrowth of blood vessels, Schwann cells, and axons could be enhanced. In the first two experimental groups, collagen nerve guides were filled with either saline or Matrigel. Marrow-derived mesenchymal stem cells (MSCs) were added to Matrigel in two other groups, one of which contained cells transfected with VEGF (MSC/VEGF). After 21 days, the injury site was exposed, fixed, sectioned, and volume fractions of the conduit contents were determined by point counting. The bioresorbable collagen conduits appropriately guided the axons and vessels in a longitudinal direction. The volume fraction of axons was significantly greater in the group with saline when compared with all three groups with Matrigel. This measure had a significant positive correlation with actual counts of myelinated axons. The blood vessel volume fraction in the Matrigel group decreased compared with the saline group, but was restored in the MSC/VEGF group. All Matrigel groups had comparable cellularity and showed a distribution of residual Matrigel in acellular zones. The saline group, by contrast, sustained a network of delicate fibroblastic processes that compartmentalized the nerve and its natural matrix as it became infiltrated by axons as minifascicles. In conclusion, the reduction of axonal outgrowth in the Matrigel groups, when compared with the saline group, suggests that Matrigel may impede the early regenerative process even when enriched by the addition of MSCs or VEGF-transfected cells.

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Neuro tissue engineering of glial nerve guides and the impact of different cell types

Cited by 139 publications

References 52 publications

The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation

The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation

Directing neuronal cell growth on implant material surfaces by microstructuring

Assessment of Axonal Growth into Collagen Nerve Guides Containing VEGF‐Transfected Stem Cells in Matrigel

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