2015
DOI: 10.1161/strokeaha.114.007834
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New Grading System Based on Magnetic Resonance Imaging in a Mouse Model of Subarachnoid Hemorrhage

Abstract: Background and Purpose A grading system for experimental subarachnoid hemorrhage (SAH) that does not require animal euthanasia is currently unavailable. We proposed a new grading system based on magnetic resonance imaging (MRI), and evaluated the feasibility of this method in a mouse model of SAH. Methods SAH was induced by endovascular perforation in adult male C57BL/6 mice. Mice underwent MRI 24 hours after SAH, and were categorized into the following five grades based on T2*-weighted imaging: Grade 0, no … Show more

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Cited by 23 publications
(13 citation statements)
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“…The mortality in this study was 19% (7/37) at 24 h after SAH induction, which is in agreement with previously published data [ 7 , 9 , 12 , 17 ]. Among the total of 30 mice, three (10%) were scored as grade 0, six (20%) as grade 1, six (20%) as grade 2, eight (27%) as grade 3, and seven (23%) as grade 4 for initial evaluation using T2*-coronal slices.…”
Section: Resultssupporting
confidence: 93%
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“…The mortality in this study was 19% (7/37) at 24 h after SAH induction, which is in agreement with previously published data [ 7 , 9 , 12 , 17 ]. Among the total of 30 mice, three (10%) were scored as grade 0, six (20%) as grade 1, six (20%) as grade 2, eight (27%) as grade 3, and seven (23%) as grade 4 for initial evaluation using T2*-coronal slices.…”
Section: Resultssupporting
confidence: 93%
“…All MR data were acquired under isoflurane anesthesia within 24 h after SAH induction, using a 4.7 Tesla Varian MRI scanner (Varian Inc., Palo Alto, CA, USA) equipped with a 12-cm internal diameter gradient coil and a 72-mm internal diameter cylindrical probehead. Whole-brain MRI was performed to ensure correct head positioning with acquisition of T2* GRE sequences (long repetition time/short echo time/flip angle=20 ms/10 ms/20°) with a field of view of 20×20×20 mm, matrix size of 128×128×128 mm, and 25 coronal slices (0.5-mm thick), as described previously [ 12 ]. An MIP was then generated from a 3-D reconstruction of the T2*-weighted slices using a free GUI-based analysis tool to support DICOM image conversion into NIfTI format (MRIcron [ 15 ]).…”
Section: Methodsmentioning
confidence: 99%
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“…The depiction of blood within the subarachnoid space using conventional imaging techniques such as magnetic resonance imaging (MRI) and computed tomography in small animals like rodents is challenging. A grading system for eSAH based on 7 Tesla MRI was already described using the endovascular perforation model in mice and rats [14][15][16]. However, a high field MRI is not available in the majority of research centers.…”
Section: Introductionmentioning
confidence: 99%