New Purinyl-Steroid and Other Constituents from the Marine Fungus Penicillium brefeldianum ABC190807: Larvicidal Activities against Aedes aegypti

Abstract

Mosquitoes historically threatened human health; the major mosquito-related global health issues include malaria, dengue fever, yellow fever, and Zika, as well as several other vector-borne outbreaks. Here, the EtOAc extract of the mangrove sediment fungus Penicillium brefeldianum ABC190807 exerted larvicidal activities against the third instar larvae of Aedes aegypti with an LC50 of 0.089 mg/mL. One new purinyl-steroid (ergosta-4,6,8(14),22-tetraen-3-(6-amino-9H-purin-9-yl) (1)), along with six (2–7) known compounds, were isolated from the EtOAc extract of Penicillium brefeldianum ABC190807. Structures of the compounds were elucidated via 1D/2D NMR and HR-ESI-MS data. Respective spectral data were compared with those of known compounds. Among all compounds whose larvicidal activity against the third instar larvae of Aedes aegypti was evaluated, compounds 2 and 7 showed larvicidal activity with respective LC50 values of 0.452 and 0.337 mg/mL.

Marine natural products

“…319 The rst naturally occurring ergosterol purine hybrid 831 was isolated from Penicillium brefeldianum. 320 Six new 4-quinolone alkaloids oxypenicinolines A-D 832-839, penicinoline F 840 and penicinoline G 841 were isolated from Penicillium steckii. Oxypenicinolines A-D, all isolated as racemates, were separated into their enantiomers by chiral HPLC.…”

Prospecção química, avaliação biológica e uso de redes moleculares como ferramenta na busca por substâncias bioativas de microrganismos marinhos

“…Em estudo recente publicado por Hou et al (2021), o extrato AcOEt do fungo marinho Penicillium brefeldianum mostrou atividade larvicida promissora contra A. aegypti (CL50 de 0,089 mg mL -1 ) e as substâncias isoladas, paspaline e fumitremorgina A apresentaram valores de CL50 de 0,452 e 0,337 mg mL -1 , respectivamente.…”