New Valve and Bonding Designs for Microfluidic Biochips Containing Proteins

Lu, Chunmeng; Xie, Yizhen; Yang, Yong Suk; Cheng, Mark Ming Cheng; Koh, Chee Guan; Bai, Yunling; Lee, L. James; Juang, Yi Je

doi:10.1021/ac0615798

Cited by 60 publications

(42 citation statements)

References 39 publications

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“…Depending on the desired functionality, a wide spectrum of hierarchical morphologies has been obtained and combined with various functional chemistries, giving rise to the first biomimetic microfluidic devices. [21][22][23] In this context, manufacturing schemes enabling the controlled and reproducible structuring of materials at micro-and nanoscales combined with surface functionalization methods are very desirable.…”

Section: Tailoring the Wetting Response For Microfluidic Applicamentioning

confidence: 99%

Biomimetic micro/nanostructured functional surfaces for microfluidic and tissue engineering applications

2011

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1 This paper reviews our work on the application of ultrafast pulsed laser micro/ nanoprocessing for the three-dimensional ͑3D͒ biomimetic modification of materials surfaces. It is shown that the artificial surfaces obtained by femtosecond-laser processing of Si in reactive gas atmosphere exhibit roughness at both micro-and nanoscales that mimics the hierarchical morphology of natural surfaces. Along with the spatial control of the topology, defining surface chemistry provides materials exhibiting notable wetting characteristics which are potentially useful for open microfluidic applications. Depending on the functional coating deposited on the laser patterned 3D structures, we can achieve artificial surfaces that are ͑a͒ of extremely low surface energy, thus water-repellent and self-cleaned, and ͑b͒ responsive, i.e., showing the ability to change their surface energy in response to different external stimuli such as light, electric field, and pH. Moreover, the behavior of different kinds of cells cultured on laser engineered substrates of various wettabilities was investigated. Experiments showed that it is possible to preferentially tune cell adhesion and growth through choosing proper combinations of surface topography and chemistry. It is concluded that the laser textured 3D micro/ nano-Si surfaces with controllability of roughness ratio and surface chemistry can advantageously serve as a novel means to elucidate the 3D cell-scaffold interactions for tissue engineering applications.

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Section: Tailoring the Wetting Response For Microfluidic Applicamentioning

confidence: 99%

Biomimetic micro/nanostructured functional surfaces for microfluidic and tissue engineering applications

2011

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“…Typically non-actuated valves having moving parts cannot be opened or closed without changing the geometry of flow paths such as with check valves, pressure control valves or flap valves. The most interesting valves for combination with CSs are non-actuated valves that have no moving parts such as hydrophobic valves (Andersson et al 2001;Lu et al 2007), diffuser valves (Stemme and Stemme 1993), pH-sensitive valves (Beebe et al 2000), and capillary valves (Man et al 1998;Leu and Chang 2004;Siljegovic et al 2005;Cho et al 2007;Chen et al 2007), for example. These valves are for single use: they influence how a liquid fills the region of the valve but not how a liquid afterward flows through the valve.…”

Section: Introductionmentioning

confidence: 99%

Valves for autonomous capillary systems

Zimmermann

Hunziker

Delamarche

2008

Microfluid Nanofluid

157

153

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Autonomous capillary systems (CSs) are microfluidic systems inside which liquids move owing to capillary forces. CSs can in principle bring the high-performances of microfluidic-based analytical devices to near patient and environmental testing applications. In this paper, we show how wettable capillary valves can enhance CSs with novel functionalities, such as delaying and stopping liquids in microchannels. The valves employ an abruptly changing geometry of the flow path to delay a moving liquid filling front in a wettable microchannel. We show how to combine delay valves with capillary pumps, prevent shortcuts of liquid along the corners of microfluidic channels, stop liquids filling microchannels from a few seconds to over 30 min, trigger valves using two liquid fronts merging, and time a liquid using parallel microfluidic paths converging to a trigger valve. All together, these concepts should add functionality to passive microfluidic systems without departing from their initial simplicity of use.

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“…Polymers, due to the simpler processing steps, less expensive materials, and good processibility for mass production and recyclability, have attracted a great deal of attention in microfabrication (Lu et al, 2005;Marie et al, 2006). To date, several polymer-based ELISA microfluidic devices have been developed based on immunoreaction on the surface of a single microchannel (Eteshola and Leckband, 2001;Lu et al, 2007) or on microbeads that are trapped in the microchannel (Herrmann et al, 2007;Sato et al, 2004). Such ELISA microdevices present several advantages over existing technologies including simplification of procedures, reduced assay time, and lower consumption of samples and reagents (Sato et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Protein A‐based antibody immobilization onto polymeric microdevices for enhanced sensitivity of enzyme‐linked immunosorbent assay

Yuan

Lee

2008

Biotech & Bioengineering

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Highly efficient antibody immobilization is extremely crucial for the development of high-performance polymeric microdevices for enzyme-linked immunosorbent assay (ELISA). In this article, a site-selective tyrosinase (TR)-catalyzed protein A strategy for antibody immobilization was developed to enhance the sensitivity of ELISA in poly-(methyl methacrylate) (PMMA) microchannels for interferon-gamma (IFN-gamma) assay. To effectively immobilize the target antibodies, oxygen plasma was first used to activate the inert PMMA. This is followed by poly(ethyleneimine) (PEI) coating, an amine-containing functional polymer. For comparison, protein A was also immobilized through the commonly used amine-glutaraldehyde (GA) chemistry. Oxygen plasma treatment effectively increased the amount of PEI attachment and subsequent binding efficiency of the primary antibody. The antibody immobilized via TR-catalyzed protein A was able to provide much better specific antigen capture efficiency than GA chemistry due to the optimal spacing and orientation. Consequently, by using this new method, the detection signal and the signal-to-noise ratio of the ELISA immunoassay in microdevices were all significantly improved. In comparison to the standard assay carried out in the 96-well microtiter plate, the treated microchannels exhibited a broader detection range and a shorter detection time. And the detection limit was also decreased to 20 pg/mL, much lower than that obtained in other microdevices.

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New Valve and Bonding Designs for Microfluidic Biochips Containing Proteins

Cited by 60 publications

References 39 publications

Biomimetic micro/nanostructured functional surfaces for microfluidic and tissue engineering applications

Biomimetic micro/nanostructured functional surfaces for microfluidic and tissue engineering applications

Valves for autonomous capillary systems

Protein A‐based antibody immobilization onto polymeric microdevices for enhanced sensitivity of enzyme‐linked immunosorbent assay

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