Nitric oxide production by haemocytes from Mytilus galloprovincialis shows seasonal variations

Novas, A. J.; Barcia, Ramiro; Ramos-Martı́nez, Juan Ignacio

doi:10.1016/j.fsi.2007.04.007

Cited by 27 publications

(22 citation statements)

References 39 publications

(37 reference statements)

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“…This result agrees with previous results (28) in the sense that they suggest that NO production varies seasonally, apparently in parallel with the variation observed in catecholamines (27).…”

Section: Discussionsupporting

confidence: 93%

“…In this sense, it is important to highlight the seasonal differences in the expression of two proteins detected by anti iNOS and anti eNOS from vertebrates in hemocytes from Mytilus galloprovincialis, which seem to be related to NO synthesis (28). The present work speculates about the involvement of PKC in the mechanisms of the seasonal regulation of NO synthesis.…”

Section: Introductionmentioning

confidence: 69%

“…The total NO released by the cell culture was determined by measuring the accumulation with Griess reagent (29) adapted to mussel hemocytes (17,28) as follows: 50 ml of culture medium was incubated with 0.2 units/ml of nitrite reductase in the presence of 2 mM NADPH (time 0). After 24 h, Griess reagent was used to determine nitrite as follows: 10 mM sulfanilic acid and 20 mM HCl (final concentrations) were added to the haemocytes.…”

Section: No Determinationmentioning

confidence: 99%

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Implication of PKC in the seasonal variation of the immune response of the hemocytes of Mytilus galloprovincialis Lmk. and its role in interleukin‐2‐induced nitric oxide synthesis

2007

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SummaryThe hemocytes are the cells responsible for the immune response in marine mollusks. The role of NO in processes related to the activation of the hemocytes has turned out evident over the late years. In the case of the mussel Mytilus galloprovincialis Lmk., hemocyte NO basal production varies throughout the year, showing a maximum in summer and a minimum in winter. IL-2 reverts the low winter NO basal production through a process mediated by cAMP-dependent protein kinase and by an apparent side effect of protein kinase C. The seasonal variation of NO production in the presence of the PKC inhibitor bisindolylmaleimide (BSM) allows suggesting a model in which PKC would modulate the activity of the enzymes responsible for nitric oxide production.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 69%

Section: No Determinationmentioning

confidence: 99%

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Implication of PKC in the seasonal variation of the immune response of the hemocytes of Mytilus galloprovincialis Lmk. and its role in interleukin‐2‐induced nitric oxide synthesis

2007

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“…Hemocytes perform various crucial immune functions, e.g., killing potential pathogens and phagocytosis of foreign materials [3]. Phagocytosis is also associated with the production of reactive oxygen species (ROS) and intermediate compounds with high bactericidal activity [4].…”

Section: Introductionmentioning

confidence: 99%

Characterization of subpopulations and immune-related parameters of hemocytes in the green-lipped mussel Perna viridis

Wang

Chiang

et al. 2012

Fish & Shellfish Immunology

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“…Hemocytes found in hemolymph and infiltrating tissues, constitute a heterogeneous population of immune cells actively specialized in pleiotropic defences against pathogens (Carballal et al 1997;Donaghy and Volety 2011;Le Foll et al 2010). During the inflammatory response, activated hemocytes execute several major functions of cell-mediated innate immunity, including cytotoxic production of oxygen (Costa et al 2009;Winston et al 1996) or nitrogen species (Novas et al 2007), increased production of lysozymes ) and phagocytosis (Carballal et al 1997;Garcia-Garcia et al 2008;Le Foll et al 2010;Malagoli et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Cell tracking and velocimetric parameters analysis as an approach to assess activity of mussel (Mytilus edulis) hemocytes in vitro

Rioult

Lebel²,

Foll

2013

Cytotechnology

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Hemocytes constitute the key element of innate immunity in bivalves, being responsible for secretion of antimicrobial peptides and release of zymogens from the prophenoloxidase system within the hemolymph compartment, reactive oxygen species production and phagocytosis. Hemocytes are found (and collected) as cells in suspension in circulating hemolymph. Hemocytes are adherent cells as well, infiltrating tissues and migrating to infected areas. In the present study, we applied an approach based on fluorescent staining and nuclei-tracking to determine migration velocity of hemocytes from the blue mussel, Mytilus edulis, in culture. Freshly collected hemocytes attached to substrate and start to move spontaneously in few minutes. Two main hemocyte morphologies can be observed: small star-shaped cells which were less motile and spread granular cells with faster migrations. Cell-tracking was combined to MTT mitochondria metabolic rate measurements in order to monitor global cell population activity over 4 days of culture. A transient peak of cell activity was recorded after 24-48 h of culture, corresponding to a speed up of cell migration. Videomicroscopy and cell tracking techniques provide new tools to characterize activity of mussel immunocytes in culture. Our analysis of hemocyte migration reveals that motility is very sensitive to cell environmental factors.

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Nitric oxide production by haemocytes from Mytilus galloprovincialis shows seasonal variations

Cited by 27 publications

References 39 publications

Implication of PKC in the seasonal variation of the immune response of the hemocytes of Mytilus galloprovincialis Lmk. and its role in interleukin‐2‐induced nitric oxide synthesis

Implication of PKC in the seasonal variation of the immune response of the hemocytes of Mytilus galloprovincialis Lmk. and its role in interleukin‐2‐induced nitric oxide synthesis

Characterization of subpopulations and immune-related parameters of hemocytes in the green-lipped mussel Perna viridis

Cell tracking and velocimetric parameters analysis as an approach to assess activity of mussel (Mytilus edulis) hemocytes in vitro

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