NOS-like immunoreactive neurons express GABA-like immunoreactivity in rabbit and rat retinae

Oh, Su-Ja; Kim, I.-B.; Lee, M.-Y.; Chun, Myung-Hoon; Chung, Jin-Woong

doi:10.1007/s002210050383

Cited by 33 publications

(30 citation statements)

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“…This assumption has been corroborated by the fact that NADPH-d is colocalized with NOS in several neuronal systems Perez et al, 1995) and also by the fact that an NADPH-d reaction is not detected in the retinae of mice devoid of the neuronal NOS gene (Darius et al, 1995). Thus, NADPH-d histochemistry (Sandell, 1985;Sagar, 1986Sagar, , 1990Mü ller et al, 1988;Cobcroft et al, 1989;Mitrofanis, 1989;Osborne et al, 1993;Lau et al, 1994) and immunocytochemical methods using anti-NOS antisera Koistinaho et al, 1993;Yamamoto et al, 1993;Oh et al, 1998Oh et al, , 1999Chun et al, 1999;Kim et al, 1999) have been applied to study the morphology and topographical distribution of NOS cells in the mammalian retina.…”

Section: Introductionmentioning

confidence: 89%

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Neuronal nitric oxide synthase immunoreactive neurons in the mammalian retina

Kim

Chun

2000

Microsc. Res. Tech.

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Section: Introductionmentioning

confidence: 89%

“…No colocalization of nNOS and VIP immunoreactivity was observed in the rat retina (Perez et al, 1995). Using double-labeling techniques, the colocalization of NADPH-d or NOS and GABA has been studied in the cat, rat, and rabbit retinae (Mü ller et al, 1988;Vaney and Young, 1988;Oh et al, 1998;see Fig. 6).…”

Section: Colocalization With Othermentioning

confidence: 95%

Neuronal nitric oxide synthase immunoreactive neurons in the mammalian retina

Kim

Chun

2000

Microsc. Res. Tech.

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“…Several somata (arrowheads) of displaced amacrine cells are visible. Bar 50 µm toreceptor cells in the rat retina, whereas no NOS immunoreactivity has been observed in these cells in the bovine, rabbit, and rat retinae (Yamamoto et al 1993;Perez et al 1995;Gotzes et al 1998;Oh et al 1998;Chun et al 1999). Accordingly, NO-containing cells might vary from one species to another, or NO-regulated mechanisms in the retina might be more complex.…”

Section: Introductionmentioning

confidence: 84%

“…NADPH-d histochemistry (Sandell 1985;Sagar 1986Sagar , 1990Müller et al 1988;Cobcroft et al 1989;Mitrofanis 1989;Osborne et al 1993;Lau et al 1994) and immunocytochemical methods with anti-NOS antisera Koistinaho et al 1993;Yamamoto et al 1993;Oh et al 1998;Chun et al 1999) have been applied to study the morphology and topographical distribution of NOS cells in the mammalian retina. According to the results obtained so far, certain types of amacrine cells and some displaced amacrine cells are consistently labeled.…”

Section: Introductionmentioning

confidence: 99%

Morphology and synaptic connectivity of nitric oxide synthase-immunoreactive neurons in the guinea pig retina

Kim

et al. 1999

Cell and Tissue Research

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Immunocytochemical methods with an antiserum against neuronal nitric oxide synthase (NOS) were applied to identify the morphology and synaptic connectivity of NOS-like immunoreactive neurons in the guinea pig retina. In the present study, two types of amacrine cells were labeled with anti-NOS antisera. Type 1 cells had large somata located in the inner nuclear layer (INL) with long, sparsely branched processes ramifying mainly in stratum 3 of the inner plexiform layer (IPL). The somata of type 2 cells (smaller diameters) were located in the INL. Some displaced amacrine cells in the ganglion cell layer were labeled. The soma size of the displaced amacrine cells was similar to that of the type 2 amacrine cells. However, processes originating from type 2 amacrine cells and displaced amacrine cells stratified mainly in strata 1 and 5, respectively. Some cone bipolar cells were weakly NOS-immunoreactive. The synaptic connectivity of NOS-like immunoreactive amacrine cells was identified in the IPL by electron microscopy. NOS-labeled amacrine cell processes received synaptic input from other amacrine cell processes and bipolar cell axon terminals in all strata of the IPL. The most frequent postsynaptic targets of NOS-immunoreactive amacrine cells were other amacrine cell processes. Cone bipolar cells were postsynaptic to NOS-labeled amacrine cells in all strata of the IPL. Labeled amacrine cells synapsing onto ganglion cells were found only in sublamina b. A few synaptic contacts were observed between labeled cell processes. In the outer plexiform layer, dendrites of labeled bipolar cells made basal contact with cone pedicles or formed a synaptic triad opposed to a synaptic ribbon of cone pedicles.

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“…In the mammalian retina, most retinal neurons containing NOS are presumed to be amacrine cells, although there is considerable variation [Perez et al, 1995;Oh et al, 1998;Kim et al, 1999;Chun et al, 1999]. In the rat retina, studies using NADPH-diaphorase (NADPH-d) histochemistry [Mitrofanis, 1989] and immunocytochemistry using anti-NOS antisera [Patel et al, 1997;Kim et al, 2000] have shown that putative NO-synthesizing cells first appear at P3 or P5 and that these cells are fully developed by the second postnatal week, before the eyes open.…”

Section: Introductionmentioning

confidence: 99%

The Effect of <i>L</i>-Arginine, a Nitric Oxide Synthase Substrate, on Retinal Cell Proliferation in the Postnatal Rat

Kim

Moon²,

Lee³

et al. 2002

Dev Neurosci

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The effects of L-arginine, a nitric oxide synthase (NOS) substrate, on cell proliferation in the developing postnatal rat retina were studied by immunocytochemistry using anti-bromodeoxyuridine (BrdU) antiserum, and by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL). Densitometric analysis by immunoblotting confirmed that neuronal NOS expression significantly increased in the L-arginine-treated retinas in comparison with the control retinas at postnatal day (P) 5 to P10. In the retinas of the control and L-arginine-treated rats, BrdU-labeled cells were only seen in the neuroblastic layer of the retinas up to P7. BrdU-labeled cells were significantly more numerous in the retinas of L-arginine-treated rats than in control retinas at P5 in the central retina and at P5 and P7 in the peripheral retina. In addition, TUNEL-positive apoptotic cells were more numerous in the retinas of L-arginine-treated rats at P5 and P7. Our results suggest that NO might play an important role in retinal maturation through regulating proliferative phases in the early stages of rat postnatal development.

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NOS-like immunoreactive neurons express GABA-like immunoreactivity in rabbit and rat retinae

Cited by 33 publications

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Neuronal nitric oxide synthase immunoreactive neurons in the mammalian retina

Neuronal nitric oxide synthase immunoreactive neurons in the mammalian retina

Morphology and synaptic connectivity of nitric oxide synthase-immunoreactive neurons in the guinea pig retina

The Effect of <i>L</i>-Arginine, a Nitric Oxide Synthase Substrate, on Retinal Cell Proliferation in the Postnatal Rat

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