Novel Immune TiO2 Photoluminescence Biosensors for Leucosis Detection

Optical biosensors based on ZnO nanostructures: advantages and perspectives. A review

“…The principle of PL biosensor action is based on the changing of PL signal before and after immobilization of biological selective sites as well as after interaction with analyte. Recently, novel immune PL biosensors based on TiO2 nanoparticles or nanowires have been developed, for the diagnostics of retroviral leucosis [60] and for the detection of sallmonella spp. [61] which were highly sensitive and selective.…”

“…There were proposed several possible mechanisms of interaction: charge transfer [34], resonance energy transfer [53], hydrophobic and electrostatic interaction [32,39,60,62].…”

Interaction mechanism between TiO2 nanostructures and bovine leukemia virus proteins in photoluminescence-based immunosensors

“…More detailed description of deposition and characterization procedures, which were applied for the characterization of nanostructured TiO 2 /glass layers is reported in earlier our researches. 18,19 Optical characterization of the nanostructured TiO 2 /glass layers was performed by photoluminescence measurements using 355 nm solid state laser as the excitation source. Optical setup is described in detail in previous our researches.…”

“…Optical setup is described in detail in previous our researches. 19,20 The PL-spectra were recorded in the range of wavelength from 360 to 800 nm.…”

“…The changes in the photoluminescence spectra (shi of PLmaximum and the variation of PL-signal intensity) were exploited as analytical signals for the determination of target analyte. 18,19 However the interaction mechanism of proteins with TiO 2 and the origin of the changes in the photoluminescence spectra were not discussed, although the mechanism of the interaction between semiconductor and proteins is the key in solving many of problems, which are still arising during the development of TiO 2 -based immunosensors, such as an improvement of sensitivity and selectivity. 3,13,14 This work is aiming to highlight the origin of the changes in the photoluminescence spectra of TiO 2 resulted aer the protein adsorption on its surface during the formation of biosensitive layer and aer the interaction of biosensitive layer with the analyte.…”