Novel one-pot synthesis and characterization of bioactive thiol-silicate nanoparticles for biocatalytic and biosensor applications

Abstract

A novel one-pot neutral synthesis using bioinspired polymers to fabricate thiol-nanoparticles is presented. The thiol-particles may be directly tethered to metal surfaces such as gold, allowing the production of self-assembled nanostructured biocatalytic or biosensor surfaces. This one-pot method has also been used to entrap enzymes within the thiol-nanoparticles; it is apparent that once enzyme entrapment is carried out a bimodal distribution of particles is formed, with particles of one mode being very similar in size to thiol-nanoparticles without enzyme entrapped, and particles of the other mode being much larger in size. To this end, efforts have been made to separate the two modes of particles for the sample containing enzyme and it has been observed that the larger mode thiol-nanoparticles do indeed contain significant amounts of enzyme in comparison to the smaller mode ones. As the enzyme-containing thiol-nanoparticles can now be isolated, this means that there are many future possibilities for the use of thiol-particles containing enzyme, as they may be used in a wide range of processes and devices which require catalytic functionalized surfaces, such as biosensors and biocatalytic reactors.

Green synthesis of silver nanoparticle by cauliflower extract: characterisation and antibacterial activity against storage

“…The main goals of the synthesis of nanoparticles are controlling over particle size, shape, and crystalline nature, i.e. the practical chemistry, which has potential applications, such as biosensor, bio‐medical, catalyst for bacterial bio‐toxin elimination, and lower cost electrode [5, 6]. The preparation of nanoparticles with a variety of morphology and sizes can be synthesised by different methods, which have been employed in chemical, biological, and physical methods.…”

High-resolution analysis of the influence of reactant concentration on nucleation time and growth of polyethyleneimine-trimethoxymethylsilane particles

“…An advantage of the particles made with TMOMS and PEI is that the presence of PEI in PEI-silica particles may be applied to carbon dioxide capture from the air [22,31], separation applications [32], and gene delivery [33]. The synthesis of PEI-silica particles which are produced by a one-pot method may have several useful applications since they are PEI-modified without a further step for surface modification being required [34].…”

Citrus ichangensisPeel Extract Exhibits Anti-Metabolic Disorder Effects by the Inhibition of PPARγand LXR Signaling in High-Fat Diet-Induced C57BL/6 Mouse

“…Regretfully, these wastes are abundant sources of natural bioactive compounds [15]. In China, citrus peels like chenpi (dried peels of mature C. reticulate ) or qingpi (dried peels of immature tangerine ( C. reticulate )) have been commonly used in Chinese medicine for the treatment of a number of diseases, such as indigestion, bronchial asthma, vomiting, cough, skin inflammation, and muscle pain [16, 17]. Moreover, citrus peels have been extensively consumed as baked products, culinary seasonings, preserves, and food supplements in China for centuries.…”