Novel poly(3‐hydroxybutyrate) composite films containing bioactive glass nanoparticles for wound healing applications

Abstract

Bioactive glass (BG) is considered an ideal material for haemostasis as it releases Ca 2+ ions upon hydration, which is required to support thrombosis. In this study the effect of the presence of the BG nanoparticles in P(3HB) microsphere films on the structural properties, thermal properties and biocompatibility of the films were studied. The nanoscaled bioactive glass with a high surface area was also tested for its in vitro haemostatic efficacy and was found to be able to successfully reduce the clot detection time. In an effort to study the effect of the roughness induced by the formation of HA on the cellular functions such as cell adhesion, cell mobility and cell differentiation, the composite films were immersed in SBF for a period of 1, 3 and 7 days. From the SEM images the surface of the P(3HB)/n-BG composite microsphere films appeared fairly uniform and smooth on day 1, however on day 3 and day 7 a rough and uneven surface was observed. The presence of HA on the composite microsphere films on day 3 and day 7 influenced the surface roughness of the films. However, when the P(3HB)/n-BG 2 composite microspheres with enhanced surface roughness were tested for biocompatibility, reduced amount of protein adsorption and cell adhesion were observed. This study thus revealed that there is an optimal surface roughness for the P(3HB) microsphere films for increased cell adhesion, beyond which it could be deleterious for cell adhesion and differentiation.

Biosynthesis and characterization of a novel, biocompatible medium chain length polyhydroxyalkanoate by Pseudomonas mendocina CH50 using coconut oil as the carbon source

“…This is higher than that reported in the literature for PHAs. The average protein adsorption for PHAs was within the range 0.12-0.35mg/cm 2 [45][46][47]. In another study , Rai et al, 2010 reported the amount of serum proteins adsorbed on the P(3HO) films to be 0.083 mg/cm 2 [8].…”

Synthesis and study of a new biopolymer‐based chitosan/hematoxylin grafted to cotton wound dressings

“…Recently a new class of dressings based on polymeric composite films, containing bioactive glass nanoparticles, appeared. These materials have been shown to induce haemostatic effect and optimization of tissue regeneration …”

Biodegradable and Biocompatible Polyhydroxy-alkanoates (PHA): Auspicious Microbial Macromolecules for Pharmaceutical and Therapeutic Applications

“…The authors assumed that increased surface porosity and roughness, important parameters for osteoblast attachment on biomaterials, and decreased crystallinity caused by incorporation of gelatin is favorable for cell growth if compared with neat PHBHHx, thus making it more suitable as material for medical implantation [ 51 ]. Other studies describe the utilization of composites of PHA and inorganic materials such as bioactive glass [ 68 , 69 ], ceramics, or hydroxyapatite for tissue engineering to improve mechanical properties, degradation rate, and to impart bioactivity; these inorganic phases can be applied either as filler material in the PHA matrix, or as coating materials (reviewed by [ 30 ]).…”

“…Studying the effect of the particle roughness caused by hydroxyapatite formation on cell adhesion, cell differentiation, and cell mobility was carried out by immersing the composite films in simulated body fluid up to one week; after some days, the surface became rough and uneven. When testing the biocompatibility of the composites with enhanced surface roughness, lower protein adsorption capacity and reduced cell adhesion were observed, indicating that surface roughness of such nanoparticles has a zenith that should not be surpassed in order to prevent deleterious effects on cell adhesion and differentiation [ 68 ]. Using a 3D-bioplotter, the team of Zhao et al produced 3D-scaffolds of composites of PHBHHx and mesoporous bioactive glass.…”